Medlinwallace8357

Herpesviruses encode multiple glycoproteins required for different stages of viral attachment, fusion, and envelopment. The protein encoded by the human cytomegalovirus (HCMV) open reading frame UL116 forms a stable complex with glycoprotein H that is incorporated into virions. However, the function of this complex remains unknown. Herein, we characterize R116, the rat CMV (RCMV) putative homolog of UL116. Two R116 transcripts were identified in fibroblasts with three proteins expressed with molecular weights of 42, 58, and 82 kDa. R116 is N-glycosylated, expressed with late viral gene kinetics, and is incorporated into the virion envelope. RCMV lacking R116 failed to result in productive infection of fibroblasts and siRNA knockdown of R116 substantially reduced RCMV infectivity. Complementation in trans of an R116-deficient virus restored ability of the virus to infect fibroblasts. Finally, UL116 knockdown also decreased HCMV infectivity indicating that R116 and UL116 both contribute to viral infectivity.

Porous silica has been extensively used as suitable carrier for the immobilization of various enzymes. Randomly Methylated β-Cyclodextrin (RaMeβCD) has surface active properties and very high solubility in water and could therefore be used as template in the fabrication of silica particles with tunable pore size.

Silica particles were prepared by sol-gel process in alkaline medium with and without use of RaMeβCD. Lipase Bfrom Candida antarctica (CALB) was either incorporated within the pores of RaMeβCD-derived support or covalently attached on the surface of CD-free silica particles and its catalytic performance was assayed in the oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA). Enzymatic reactors were characterized by N

-adsorption analysis, small angle XRD, TG/DSC experiments, ATR-FTIR spectroscopy, HR-TEM and LSCM, while reaction products were determined based on

H NMR spectroscopy combined with HPLC.

Results showed that the use of RaMeβCD as structure directing agent ledRaMeβCD-derived silica caused almost a two-fold increase in specific activity after 7 h at 40 °C when compared to lipase immobilized on the surface of CD-free silica particles (33.2 μmol g-1 min-1vs. 14.4 μmol g-1 min-1). Moreover, the RaMeβCD-derived biocatalyst demonstrated enhanced operational stability during the recycling experiments, retaining more than 90% of its initial activity after five 24 h-reaction cycles. These findings open up new avenues for future research on the use of cyclodextrins in the development of enzyme-based nanoreactors.In this article, we analyze whether the financial strain of poverty systematically alters the allocation of attention. We address two types of attention attention to unexpectedly occurring events and attention to primary tasks that require focus. see more We show that the poor are significantly more likely than the rich to notice unexpected events. In addition, we do not find robust evidence that poverty increases the likelihood of noticing the unexpected events at the expense of attention to the primary task.This article has been retracted please see Elsevier Policy on Article Withdrawal (https//www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Editor because of serious doubts regarding the data on melatonin levels. The authors used a melatonin ELISA kit that was not fit for purpose, resulting in data showing peak secretion of this hormone occurring in the middle of the light period, which does not make any physiological sense since melatonin is only produced during darkness.Occupational tasks often involve musculoskeletal demands that contribute to injury risk. In pallet wrapping tasks, 36% of workplace claims involve over exertion and repetitive exposures (Workplace Safety and Prevention Services, 2012). Tools that modify how the wrap is handled by workers have been introduced to help mitigate over exertion and extreme postures wherever possible. A novel device has been introduced that places the required tension on the roll to mitigate these factors. However, the effect of reducing the necessary tension on the roll during pallet wrapping in limiting muscular demand and extreme postures is unknown. Fourteen healthy university aged participants completed 12 wrapping trials on a simulated pallet (2 repetitions of 2 wrapping techniques (device, hand wrapping) at 3 wrapping heights (low, medium, high)). Surface electromyography (sEMG) was measured on 6 shoulder and 2 low back muscles; anterior and middle deltoids, biceps brachii, infraspinatus, supraspinatus, upper trapezius, and erector spinae (T8 & L3). Kinematic data were collected for the torso and upper extremity and global to torso, and torso to upper arm angles were computed. Repeated measures ANOVAs were performed for the following experimental factors 1) technique used (device or hand wrapping) and 2) the wrapping height (low, medium, high) for each muscle (8), angle (5), rating of perceived discomfort (1) and rating of perceived exertion (1). Pallet wrapping without a device required greater trunk flexion at lower heights and thoracohumeral elevation at higher wrapping heights as compared to using the device. Muscular activation increased when using the device, specifically at the higher and lower heights. Posture and muscular demands during pallet wrapping tasks are sensitive to both wrapping technique and wrapping height. Ergonomics interventions such as this device may mitigate postural risks associated with manual material handling tasks.

To perform the genetic characterization of a cohort with familial parkinsonism and cognitive-behavioral syndrome.

A Next Generation Sequencing - based targeted sequencing of 32 genes associated to various neurodegenerative phenotypes, plus a screening for SNCA Copy Number Variations and C9orf72 repeat expansion, was applied in a cohort of 85 Italian patients presenting with parkinsonism and cognitive and/or behavioral syndrome and a positive familial history for any neurodegenerative disorder (i.e., dementia, movement disorders, amyotrophic lateral sclerosis).

Through this combined genetic approach, we detected potentially relevant genetic variants in 25.8% of patients with familial parkinsonism and cognitive and/or behavioral syndrome. Peculiar phenotypes are described (Cortico-basal syndrome with APP, Posterior Cortical Atrophy with GBA, Progressive Supranuclear Palsy-like with GRN, Multiple System Atrophy with TARDBP). The majority of patients presented a rigid-bradykinetic parkinsonian syndrome, whienerative syndromes, highlighting the limitations of current clinical diagnostic criteria defining sharp boundaries between distinct conditions.The rational design of high-performance and low-cost oxygen evolution reaction (OER) electrocatalysts for water splitting is of vital importance for development of renewable hydrogen energy. Herein, we demonstrate an interfacial engineering strategy to prepare Fe-doped CoP nanocubes/CoP nanosheet arrays heterostructure supported on carbon cloth (denoted as CoFeP/CoP/CC). The resultant CoFeP/CoP/CC heterostructure catalyst possesses abundant heterogeneous interfaces, which enables the exposure of reaction active sites and possibly modulation of electronic structure of the catalyst. Furthermore, this strong interfacial coupling of CoFeP and CoP as well as the integration structure on the carbon cloth guarantee high electronic conductivity and enhanced mechanical stability. Benefiting from these advantages, the CoFeP/CoP/CC-heterostructure exhibits high electrocatalytic OER performance with a low overpotential of 240 mV for reaching a current density of 10 mA cm-2, which outperforms the commercial noble metal RuO2 (255 mV) and many reported TMPs-based electrocatalysts. Moreover, this CoFeP/CoP/CC catalyst shows a remarkable OER catalytic stability over 100 h. This work provides an effective avenue for the design of the high-performance OER catalyst by interfacial engineering strategy.

Water and oil inhibition treatment is essential for protecting natural and artificial stone surfaces. Bioinspired super-antiwetting surfaces with "lotus effect", together with superoleophobic properties, can be achieved combining very low-surface-energy materials and suitable surface roughness. Exploiting the natural roughness of stone surfaces, the simple and inexpensive fabrication of superamphiphobic surfaces through the coating dispersion deposition is expected. It seems the ideal method for the safeguard of contemporary and historical constructions, since the physical, chemical and aesthetic properties can be maintained.

The new coating agent (3-perfluroether-amidopropylsilane) was synthesized via one-step amidation. Hydrophobicity, robustness and environmental durability were systematically studied on stone surfaces through several tests contact angle (CA), contact angle hysteresis (CAH), water inhibition efficiency, vapor diffusivity, chemical and mechanical resistance, artificial and field-exposurction in rainy regions where acid rain is also present.Wastewater from the olive mill constitutes a serious environmental concern, as it is characterized by a high inorganic and organic load. Here, a hybrid photocatalyst based on calcined Ca-Fe-LDH was successfully synthesized for the degradation of phenolic compounds and the removal of chemical oxygen demand (COD) from the high-strength olive mill wastewater (OMW). The catalyst (CuCr2O4@CaFe-LDO) displayed a stable ~4.48 µA cm-2 photocurrent response, a 2.56 eV bandgap and a wide variety of pores with an average size of 12.51 nm. 1.0 g CuCr2O4@CaFe-LDO achieved 66% COD removal after 300 mins without an oxidant in the dark, while after 180 mins of reaction, CuCr2O4@CaFe-LDO/K2S2O8/sunlight system resulted in ~99% and 98.3% COD and colour removal. Seven phenolic compounds were found in the crude OMW, with hydroxytyrosol (76.84%) and tyrosol (15.14%) being the main ones. The final pH of the sample treated increased from 4.3 to 7.3, which confirmed the degradation of phenolics and fatty acids in the OMW. OH, SO4-, h+ and O2- contributed notably to the degradation of polyphenols and the spent catalyst was easily and rapidly recovered from the bulk solution due to its saturation magnetization of 54.7 emu g-1.Rational design of metal-free carbon-based heterogeneous catatlyst for wastewater remediation via peroxymonosulfate (PMS) activation is highly desirable. Here, hollow structured porous carbon with abundant N, a high graphitization degree, and a large specific surface area and pore volume (1301 m2/g and 1.12 cm3/g) was synthesized by the pyrolysis of core-shell structured composites consisting of polystyrene (PS) cores and Zeolitic imidazolate frameworks-8 (ZIF-8) shells. The hollow structured carbon (CPS@ZIF-8) was characterized thoroughly and applied for phenol degradation by the activation of PMS. The effects of operation conditions such as the catalyst and PMS dose, phenol concentration, initial pH, and temperature on phenol removal were investigated comprehensively. Moreover, the main reactive species involved in phenol oxidation were investigated, and a plausible mechanism for the degradation of phenol is proposed. The results show that CPS@ZIF-8 exhibited an excellent phenol adsorption and degradation performance, which can be mainly ascribed to its large surface area, abundance of nitrogen and hollow porous structure.