Mcgeehaas1603

Up-to-date investigations of tau species in peripheral tissues are scant and have mainly been restricted to rodents, whereas, more evidence is available on alpha synuclein in peripheral tissues. Here we aim to review the literature on the functional role of tau and alpha synuclein in physiological conditions and disease at the axonal level, their distribution in peripheral tissues, and discuss possible commonalities/diversities as well as their interaction in proteinopathies.Bio-based thermosetting resins were synthesized from a ternary composition humins; epoxidized linseed oil (ELO); and an industrial hardener, Capcure3-800 (CAP). Humins are in a focused attention in the last years, as biorefinery by-product, therefore its valorization through materials design is very important. Here we present a structural study of terpolymerization of humins/ ELO/CAP. The reactivity of these systems was highlighted by in situ FT-IR and 1H and 13C NMR. The integration of humins in thermosetting resins gives alternatives to new feedstocks for future bio-based materials.Human embryonic stem cells (hESCs) have important roles in regenerative medicine, but only a few studies have investigated the cytokines secreted by hESCs. We screened and identified chemokine (C-X-C motif) ligand 14 (CXCL14), which plays crucial roles in hESC renewal. CXCL14, a C-X-C motif chemokine, is also named as breast and kidney-expressed chemokine (BRAK), B cell and monocyte-activated chemokine (BMAC), and macrophage inflammatory protein-2γ (MIP-2γ). Knockdown of CXCL14 disrupted the hESC self-renewal, changed cell cycle distribution, and further increased the expression levels of mesoderm and endoderm differentiated markers. Interestingly, we demonstrated that CXCL14 is the ligand for the insulin-like growth factor 1 receptor (IGF-1R), and it can activate IGF-1R signal transduction to support hESC renewal. Currently published literature indicates that all receptors in the CXCL family are G protein-coupled receptors (GPCRs). This report is the first to demonstrate that a CXCL protein can bind to and activate a receptor tyrosine kinase (RTK), and also the first to show that IGF-1R has another ligand in addition to IGFs. These findings broaden our understanding of stem cell biology and signal transduction.Faithful chromosome segregation is essential for the maintenance of genomic integrity and requires functional centromeres. Centromeres are epigenetically defined by the histone H3 variant, centromere protein A (CENP-A). Here we highlight current knowledge regarding CENP-A-containing chromatin structure, specification of centromere identity, regulation of CENP-A deposition and possible contribution to cancer formation and/or progression. CENP-A overexpression is common among many cancers and predicts poor prognosis. Overexpression of CENP-A increases rates of CENP-A deposition ectopically at sites of high histone turnover, occluding CCCTC-binding factor (CTCF) binding. Ectopic CENP-A deposition leads to mitotic defects, centromere dysfunction and chromosomal instability (CIN), a hallmark of cancer. CENP-A overexpression is often accompanied by overexpression of its chaperone Holliday Junction Recognition Protein (HJURP), leading to epigenetic addiction in which increased levels of HJURP and CENP-A become necessary to support rapidly dividing p53 deficient cancer cells. Alterations in CENP-A posttranslational modifications are also linked to chromosome segregation errors and CIN. Collectively, CENP-A is pivotal to genomic stability through centromere maintenance, perturbation of which can lead to tumorigenesis.Casein is often used as an eco-friendly wood adhesive. In this study, we used casein for soil cementation by mixing it with Jumunjin sand, sodium hydroxide (SH), and calcium hydroxide (CH) as a standard casein formula. The modified casein binder with different proportions of SH and CH was applied to improve water resistance. Furthermore, a blast furnace slag (BFS) was additionally mixed and reacted with alkalinity of modified casein binder. Thus, three types (standard, modified, and modified + BFS, referred to as STD, MOD, and MBS, hereafter) of casein binders were tested for durability and strength of casein-cemented sand. A piezoelectric sensor was installed within each sample to determine the curing time of the casein-cemented samples. The samples were air-cured at room temperature for seven days and some were repeatedly immersed in water thrice. read more Unconfined compression and jar slake tests were carried out to evaluate the strength and durability of the casein-cemented sand. Also, the microstructure was analyzed using a scanning electron microscope (SEM). We observed variations of peak conductance and corresponding frequency converged as the curing time increased. It was most significant for the MBS samples, which developed strength early. The unconfined compressive strength (UCS) of the air-cured samples was higher than those repeatedly immersed in water due to wash-off of the casein binder. The UCS of the dry MBS sample was 9900 kPa while that of the immersed sample was 430 kPa, which gradually decreased to 60 kPa upon repeated immersion. The samples with STD and MOD had no resistance to durability and showed cracks on the surface, while the MBS sample exhibited significantly improved durability and no cracks. We found that the MBS binder had a positively significant effect on the durability and strength of casein-cemented sand.In the current study, we present a case of an intramuscular ganglion cyst in the flexor hallucis brevis muscle (FHB) that arose secondary to a muscle tear. Through this study, we propose a possible aetiology for the development of intramuscular ganglionic cysts. A 50-year-old woman presented with acute pain and swelling over the right mid-plantar area after prolonged kneeling for scrubbing floors. Ultrasonography examination performed at 5 days after the onset of symptoms revealed a partial tear of the right FHB. Follow-up evaluations were conducted, with magnetic resonance imaging and ultrasonography, at 24 and 54 days after symptom onset. MRI revealed a ganglion cyst in the mid-portion of the FHB without connection to the adjacent joint capsule or tendon sheath. On the ultrasonography examination at 45 days after onset, at the same location where a tear was seen on the initial examination, an anechoic defect in the mid-portion of the FHB was observed, compatible with a ganglion cyst. Given the favourable natural evolution, no aspiration or surgery were performed. The patient was discharged with minimal symptoms. The results suggest that the intramuscular ganglion cyst can develop following a muscle tear.Tetraena mongolica is a rare and endangered species unique to China. The total number and density of Tetraena mongolica shrubs in desertification areas have experienced a sharp decrease with increases in coal mining activities. However, available information on the T. mongolica rhizosphere soil quality and microbial properties is scarce. Here, we investigated the effect of coal mining on the soil bacterial community and its response to the soil environment in the T. mongolica region. The results showed that the closer to the coal mining area, the lower the vegetation coverage and species diversity. The electrical conductivity (EC) in the contaminated area increased, while the total nitrogen (TN), available phosphorus (AP), available potassium (AK), and soil organic carbon (SOC) decreased. The activity of β-glucosidase, urease, alkaline phosphatase, and catalase further decreased. In addition, the mining area could alter the soil's bacterial abundance and diversity. The organic pollutant degradation bacteria such as Sphingomonas, Gemmatimonas, Nocardioides, and Gaiella were enriched in the soil, and the carbon-nitrogen cycle was changed. Canonical correspondence analysis (CCA) and Pearson's correlation coefficients showed that the change in the bacterial community structure was mainly caused by environmental factors such as water content (SWC) and EC. Taken together, these results suggested that open pit mining led to the salinization of the soil, reduction the soil nutrient content and enzyme activity, shifting the rhizosphere soil microbial community structure, and altering the carbon-nitrogen cycle, and the soil quality declined and the growth of T. mongolica was affected in the end. Therefore, the development of green coal mining technology is of great significance to protect the growth of T. mongolica.Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein that is encoded by the AIF1 gene. The main functions of AIF-1 are the activation of macrophages and enhancing the production of pro-inflammatory cytokines. To date, three different AIF-1 isoforms have been identified. In this study, we examined the expression of AIF-1 isoforms on the level of mRNA, and we compared the percentage of AIF-1-positive white blood cells (WBCs) in blood and AIF-1/CD68 cells in the synovial membranes in patients with rheumatoid arthritis (RA) and osteoarthritis (OA). We examined 15 patients with RA and 15 patients with OA who had previously undergone knee arthroplasty. Peripheral blood and synovial membranes (SMs) were collected from these patients during knee arthroplasty. We identified three AIF-1 mRNA expression variants in peripheral mononuclear cells (PBMCs) and SMs from patients in both groups. Spearman's rank correlation coefficient tests showed strong, positive, and significant correlations between the three AIFood and SMs, we observed an increased percentage of AIF-1-positive cells in patients with RA in comparison to patients with OA. The above results suggested that AIF-1 was the cytokine involved in the pathogenesis of RA. The precise knowledge of the role of AIF-1 in RA pathogenesis and the development of inflammatory response requires further investigations.

Worsening lower urinary tract symptoms (LUTS) are a frequent adverse event following proton beam therapy (PBT) for localized prostate cancer. We investigated the differences in worsening LUTS among patients who received PBT at different times of day.

Among 173 patients who underwent PBT for prostate cancer, 168 patients (median age 68.5 years) completed international prostate symptom score (IPSS) questionnaires and were included. Changes in the IPSS from baseline to the end of PBT were assessed by multiple linear regression analysis for age, National Comprehensive Cancer Network risk classification, androgen deprivation therapy, fractional PBT dose, clinical target volume, severity of IPSS, diabetes, LUTS medication use before PBT, anti-coagulant therapy and radiation time of day (morning (0830-1030), around noon (1031-1430), and late afternoon (1431-1630)).

IPSS total score and IPSS-Quality of Life (QoL) score (12 patients were excluded due to missing IPSS-QoL score) increased from eight to 14.9 (

< 0.0001) and from two to four (

< 0.0001), respectively. Time of day (morning) was the only determinant for worsening LUTS (β = -0.24,

< 0.01), voiding subscore (β = -0.22,

< 0.05) and IPSS-QoL (β = -0.27,

< 0.005), and was a determinant in item four (urgency) (β = -0.28,

< 0.005) with age (β = 0.19,

< 0.05).

Morning PBT for localized prostate cancer significantly ameliorated worsening LUTS and improved QoL compared with treatment around noon or late afternoon. Chronoradiation therapy for localized prostate cancer may be effective and further research to elucidate the underlying mechanism is warranted.

Morning PBT for localized prostate cancer significantly ameliorated worsening LUTS and improved QoL compared with treatment around noon or late afternoon. Chronoradiation therapy for localized prostate cancer may be effective and further research to elucidate the underlying mechanism is warranted.