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In response to coronavirus disease (COVID-19), the Shanghai Burn Clinical Quality Control Center organized experts to formulate and implement a set of rapid, simple, and effective prevention and control measures, and there have not been any cases of healthcare professionals or inpatients in burn units suspected or confirmed with COVID-19. This article elaborates on the specific measures in burn units in response to the epidemic, including the implementation of standardized procedures, remote consultations, strengthened follow-up, exchange of experience, and popular science, among others. We share experience from Shanghai to benefit related disciplines in other countries and regions.

Nature of Chinese Herbal Medicines (CHMs) is a bridge between traditional Chinese medicine (TCM) and clinical application. Accurate nature identification of CHMs is essential for guiding the clinical application of CHMs.

To develop a new method for nature identification of CHMs according to compounds in CHMs.

The nature of a CHM is a comprehensive manifestation of the properties of various compounds in this CHM. In this study, 2012 CHM compounds were extracted to construct a compound dataset. Molecular descriptors were utilized to build an identification model for classification of cold-hot-neutral nature of CHM compounds.

The predicting accuracy and confusion matrix were validated by the assembled dataset. The best model produced the accuracies of 96.5%±0.5% and 86.5%±1.5% on training set and test set, respectively. Furthermore, the identification model is robust in predicting hot-cold-neutral nature of the CHM compounds.

This work shows how a classification model for medical nature identification can be developed. The derived model can be utilized for the application of CHMs.

To construct a nature identification model for analysis of hot-cold-neutral nature of CHMs according to the compounds in CHMs.

To construct a nature identification model for analysis of hot-cold-neutral nature of CHMs according to the compounds in CHMs.Endovascular intervention has become the mainstay of treatment for subclavian artery stenosis in many centers, with high technical success and low complication rates.1,2 However, potential embolization during proximal subclavian artery intervention can lead to catastrophic posterior circulation ischemic complications.3-5 Although considered a rare complication, the presence of a contralateral hypoplastic vertebral artery with persisting anterograde vertebral blood flow on the affected side is likely to increase the risk of embolization.3 The use of embolic protection devices, such as filters and noncompliant balloons, has been previously described.3,6,7 However, there is still a risk of distal embolization and vessel injury with the use of these devices.7 We present a technical video of a patient in their 80s with left subclavian stenosis who underwent subclavian stent-assisted percutaneous transluminal angioplasty (SAPTA) using an anterograde-retrograde access technique with a dual-lumen compliant balloon catheter (Scepter XC; MicroVention, Aliso Viejo, California) placed at the proximal segment of the left vertebral artery. With this approach, the compliant balloon provides adequate protection while minimizing the risk of endothelial injury and distal embolization. Written informed consent was obtained for the procedure. Patient consent was waived because all health information was deidentified.Idiopathic pulmonary fibrosis (IPF) is a progressive fatal disease affecting over 100 000 people in Europe with an increasing incidence. Available treatments offer only slowing of disease progression and are poorly tolerated by patients leading to cessation of therapy. Lung transplant remains the only cure. Therefore, alternative treatments are urgently required. The pathology of IPF is complex and poorly understood and thus creates a major obstacle to the discovery of novel treatments. Additionally, preclinical assessment of new treatments currently relies upon animal models where disparities with human lung biology often hamper drug development. At a cellular level, IPF is characterized by persistent and abnormal deposition of extracellular matrix by fibroblasts and alveolar epithelial cell injury which is seen as a key event in initiation of disease progression. In-depth investigation of the role of alveolar epithelial cells in health and disease has been impeded due to difficulties in primary cell isolation and culture ex vivo. Novel strategies employing patient-derived induced pluripotent stem cells engineered to produce type 2 alveolar epithelial cells (iAEC2) cultured as three-dimensional organoids have the potential to overcome these hurdles and inform new effective precision treatments for IPF leading to improved survival and quality of life for patients worldwide.Electrical injuries comprise 4% of cases but have higher morbidity and mortality. This study aims to share our experiences with pediatric electrical injuries and propose strategies to prevent them. The files of pediatric electrical injuries between 2010-2020 were reviewed retrospectively. The following were investigated age, gender, cause, length of stay in the pediatric burn center, total burned surface area, voltage-type, and surgical procedures performed. The patients from low and high-voltage groups compared. Eighty-five patients were treated in the last ten years. Seventy were males, the mean age was 9.9 years, the average length of stay in pediatric burn center was 18.2 days, and the average total burned surface area was 11.7%. Forty-three patients were injured with high-voltage and 42 with low-voltage electricity. Selleckchem PYR-41 Fasciotomy was performed in 25 patients, grafting in 40 patients, and amputation in 12 patients. The most often amputated limb was the right arm/forearm. Psychiatric disorders developed in 24 patients. One patient died. In conclusion, the incidence of high-voltage electrical injuries increases with age. They are more prevalent in males, more often accompanied by additional trauma, and have higher total burned surface area, surgical procedures are performed more often, and hospitalization times are longer. For prevention, precautions should be taken by governments and families, and education is critical.

The Simultaneous Multiplex Real Time PCR (SIMUL-qPCR) Top 7 STEC Assay Collection is a quick, reliable method for detecting Top 7 Shiga toxin-producing E. coli (STEC) in raw beef trim, raw ground beef, and beef carcass sampling sheets. Each assay multiplexes several targets in one run to identify E. coli O157 H7, O26, O45, O103, O111, O121, O145, Shiga toxin and intimin genes. This collection uses specifically optimized proprietary media for single-step recovery and enrichment of Enterohemorrhagic E. coli (EHEC).

This report details the method validation study to validate raw beef trim, raw ground beef, and beef carcass sampling sheets.

Matrix studies for raw beef trim, raw ground beef, and beef carcass sampling sheets, inclusivity/exclusivity, product consistency/stability, and robustness testing were conducted to assess the method's performance.

50 Top 7 STEC isolates were analyzed with the SIMUL-qPCR Assay. 32 isolates, including closely related non-E. coli species and E. coli non-STEC strains, were also tested. Inclusivity showed the collection detected the Shiga toxin (stx) gene, intimin (eae) gene, and Top 7 serogroups. None of the 32 exclusivity strains were detected. The candidate and reference methods' results had no statistically significant differences during matrix studies. Small variations in critical test parameters (enrichment time, extraction reagent volume, and extracted sample volume) didn't adversely affect the assay's performance, and stability testing indicated consistent results for at least one year.

The data presented demonstrate that the SIMUL-qPCR Top 7 STEC Assay is a reliable method for detecting the Top 7 STEC.

The data presented demonstrate that the SIMUL-qPCR Top 7 STEC Assay is a reliable method for detecting the Top 7 STEC.

Artemisia hedinii is a well-known traditional Chinese medicine. It can be used to extract dihydroartemisinin (DHA).

The purpose of this study was to explore the optimal conditions for the homogenate extraction of DHA from A. hedinii and the antifungal activity of DHA.

In this study, single factor experiments and response surface method were used to determine the optimal extraction conditions of crude extract and DHA, the method of spore germination was used to study the antifungal activity of DHA to Alternaria alternata.

The optimal conditions were found as fellow ratio of liquid to material 22 mL/g; Extraction time 60 s; soaking time 34 min. Under these conditions, extraction yield of DHA was (1.76 ± 0.04%). When the concentration of crude extract were 0.5 and 8 mg/mL, the spore germination inhibition rates of Alternaria alternata were (17.00 ± 2.05%) and (92.56 ± 2.01%), which were 3.34 and 1.15 times that of DHA standard, respectively.

Homogenate extraction technology is a fast and efficient method to extract DHA from A. hedinii. The crude extract has significant antifungal activity against A. alternata with low cost, which provides a possibility for the use of DHA in the prevention and treatment of plant pathogenic fungi.

The optimum conditions of the extraction of DHA from A. hedinii by homogenate extraction were obtained. DHA has antifungal activity against A. alternata. Compared with pure DHA, the crude extract has stronger antifungal activity against A. alternata.

The optimum conditions of the extraction of DHA from A. hedinii by homogenate extraction were obtained. DHA has antifungal activity against A. alternata. Compared with pure DHA, the crude extract has stronger antifungal activity against A. alternata.Protein catabolism is a common complication after burn injury leading to loss of muscle mass and a decrease in muscle strength. The present study aims to evaluate the efficacy of combining exercise training with protein supplementation on lean body mass and muscle strength in patients with severe burn. Sixty participants with severe burn > 30% of total body surface area were randomly distributed into 4 equal groups of 15 participants per group. Group A (Exercise & Protein) received oral protein supplementation (Inkospor X-TREME; 1.5-2.0 g/kg/day), exercise program and traditional burn care, group B (Protein) received oral protein supplementation and traditional burn care, group C (Exercise) received exercise program and traditional burn care, group D (Control) received traditional burn care. Lean body mass was measured using Dual-energy x-ray absorptiometry whereas muscle strength was measured using Biodex 3 Dynamometer System before treatment and 12 weeks after treatment. A significant increase in lean body mass was found in group A compared with that of group B, group C and group D post-treatment (p less then 0.001). Also, a significant increase was recorded in peak torque of group A compared with that of group B, group C and group D post-treatment (p less then 0.001). Exercise training can significantly increase lean body mass and peak torque. Protein supplementation can significantly increase lean body mass but cannot significantly increase muscle strength whereas, protein +exercise group has a great impact on lean body mass and muscle power than exercise group and protein group in burn patients.