Luvillumsen0423

Although explicit verbal expression of prejudice and stereotypes may have become less common due to the recent rise of social norms against prejudice, prejudice in language still persists in more subtle forms. It remains unclear whether and how language patterns predict variance in prejudice across a large number of minority groups. Informed by construal level theory, intergroup-contact theory, and linguistic expectancy bias, we leverage a natural language corpus of 1.8 million newspaper articles to investigate patterns of language referencing 60 U.S. minority groups. We found that perception of social distance among immigrant groups is reflected in language production Groups perceived as socially distant (vs. close) are also more likely to be mentioned in abstract (vs. concrete) language. Concreteness was also strongly positively correlated with sentiment, a phenomenon that was unique to language concerning minority groups, suggesting a strong tendency for more socially distant groups to be represented with more negative language. We also provide a qualitative exploration of the content of outgroup prejudice by applying Latent Dirichlet Allocation to language referencing minority groups in the context of immigration. We identified 15 immigrant-related topics (e.g., politics, arts, crime, illegal workers, museums, food) and the strength of their association and relationship with perceived sentiment for each minority group. This research demonstrates how perceived social distance and language concreteness are related and correlate with outgroup negativity, provides a practical and ecologically valid method for investigating perceptions of minority groups in language, and helps elaborate the connection between theoretical positions from social psychology with recent studies from computer science on prejudice embedded in natural language.Medulloblastoma, the most common malignant brain tumor in children, consists of four molecular subgroups WNT, SHH, Group 3, and Group 4. Group 3 has the worst survival rate among the four subgroups and is characterized by the expression of retina-specific genes. CRX, the master regulator of the photoreceptor differentiation, is aberrantly expressed in Group 3 medulloblastomas. CRX expression increased the proliferation, anchorage-independent growth, invasion potential, and tumorigenicity of medulloblastoma cells indicating the oncogenic role of CRX in medulloblastoma pathogenesis. CRX knockdown resulted in the downregulation of expression of several retina-specific genes like IMPG2, PDC, RCVRN. and Group 3 specific genes like GABRA5, MYC, PROM1. Thus, CRX plays a major role not only in the expression of retina-specific genes but also in defining Group 3 identity. Increased expression of several pro-apoptotic genes upon CRX knockdown suggests that CRX could protect Group 3 medulloblastoma cells from cell death. Several negative regulators of the TGF-β signaling pathway like SMAD7, PMEPA1, KLF2 were upregulated upon the CRX knockdown. Western blot analysis showed a decrease in the levels of (Phospho)-SMAD2, total levels of SMAD2, SMAD4, and an increase in the levels of SMAD7 indicating inhibition of the TGF-β signaling pathway upon CRX knockdown. Copy number variations in several genes involved in the TGF-β signaling pathway occur in a subset of Group 3 tumors. Autocrine TGF-β/activin signaling has recently been reported to be active in a subset of Group 3 medulloblastomas. CRX knockdown resulting in the inhibition of the TGF-β/activin signaling pathway demonstrates an interaction between the two Group 3 specific oncogenic pathways and suggests simultaneous targeting of both CRX and TGF-β signaling as a possible therapeutic strategy.Rett syndrome (OMIM #312750) is a developmental neurological disorder that is caused by a mutation in methyl-CpG-binding protein 2 (MeCP2). MeCP2 localizes to the nucleus, binds to methylated DNA, and regulates gene expression during neuronal development. MeCP2 assembles multiple protein complexes and its functions are controlled by interactions with its binding partners. Therefore, functional analysis of MeCP2 binding proteins is important. Navitoclax Previously, we proposed nine MeCP2-binding candidates in the cerebral cortex. In this study, we characterized and examined the function of the MeCP2 binding protein zinc finger protein 483 (ZNF483) to determine the significance of the MeCP2-ZNF483 interaction in neuronal development. Phylogenetic profiling revealed that the ZNF483 protein is broadly conserved in metazoans. In contrast, MeCP2 was obtained during evolution to chordates. To investigate ZNF483 functions, ZNF483-knockout P19 cell lines were established using the CRISPR-Cas9 system. These cell lines showed decreased cell proliferation, altered aggregate formation, decreased neuronal marker NeuN expression, and altered MeCP2 phosphorylation patterns. Notably, cytosolic localization of MeCP2 was enhanced by ZNF483-overexpression. Taken together, we propose that ZNF483 might be involved in the promotion of neuronal differentiation by regulating the subcellular localization of MeCP2 in P19 cells.

As trends in new HIV diagnoses represent a measure of the HIV epidemic, we conducted a 6-year longitudinal study to evaluate the change in rates of new HIV diagnosis, stratified by birthplace, HIV risk groups and CD4 cell count at diagnosis in a large French multicentre cohort.

We performed a retrospective cohort study using data from the mainland French Dat'AIDS cohort.

Data were obtained for subjects with a new HIV diagnosis date between 2013 and 2018. HIV diagnosis date was defined as the date of the first known positive HIV serology.

Between 2013 and 2018, a total of 68,376 people living with HIV (PLHIV) were followed in the Dat'AIDS cohort; 9543 persons were newly diagnosed with HIV. The annual number of new HIV diagnoses decreased from 1856 in 2013, to 1149 in 2018 (-38.1%), P=0.01; it was more pronounced among subjects born in France, from 858 to 484 (-43.6%), P<0.01, than in those born abroad (-23.8%, from 821 to 626, P=0.13). Among subjects born in France, the decrease over the period was-46.