Lundingmarker9726

Our classifier may aid clinicians in deciding if a skin lesion should be biopsied and can easily be incorporated into a portable tool (that uses no proprietary equipment) that could aid clinicians in noninvasively evaluating cutaneous lesions.

Our classifier may aid clinicians in deciding if a skin lesion should be biopsied and can easily be incorporated into a portable tool (that uses no proprietary equipment) that could aid clinicians in noninvasively evaluating cutaneous lesions.

Psoriasis and migraine are common conditions with potential overlap of pathophysiological mechanisms. Both these diseases have been associated with increased cardiovascular risk but little is known about their interplay.

We sought to investigate the link between psoriasis, and the risk of new-onset migraine, in a nationwide cohort of the Danish population.

Data on all Danish citizens aged 18 years or older from January 1, 1997, to December 31, 2011, were linked at individual-level in nationwide registers. Incidence rates per 1000 person-years were calculated and crude and adjusted incidence rate ratios were estimated by Poisson regression models.

The study comprised a total of 5,379,859 individuals, including 53,006 and 6831 patients with mild and severe psoriasis, respectively, and 6243 patients with psoriatic arthritis. Fully adjusted incidence rate ratios for migraine were 1.37 (95% confidence interval 1.30-1.45), 1.55 (95% confidence interval 1.29-1.86), and 1.92 (95% confidence interval 1.65-2.22) for mild psoriasis, severe psoriasis, and psoriatic arthritis, respectively. Stratification for sex revealed increased risk of migraine in both male and female patients.

We were unable to distinguish between subtypes of migraine, eg, migraine with and without aura.

Psoriasis was associated with a disease severity-dependent increased risk of migraine independent of measured confounders. Further studies are warranted to determine the effects of antipsoriatic treatment on this association, and whether migraine modifies the psoriasis-associated risk of cardiovascular disease.

Psoriasis was associated with a disease severity-dependent increased risk of migraine independent of measured confounders. Further studies are warranted to determine the effects of antipsoriatic treatment on this association, and whether migraine modifies the psoriasis-associated risk of cardiovascular disease.Grazing profoundly influences vegetation and the subsequent carbon fluxes in various ecosystems. However, little effort has been made to explore the underlying mechanisms for phenological changes and their consequences on carbon fluxes at ecosystem level, especially under the coupled influences of human disturbances and climate change. Here, a manipulative experiment (2012-2013) was conducted to examine both the independent and interactive effects of grazing and watering on carbon fluxes across phenological phases in a desert steppe. Grazing advanced or delayed phenological timing, leading to a shortened green-up phase (GrP 23.60 days) in 2013 and browning phase (BrP 12.48 days) in 2012 from high grazing, and insignificant effects on the reproductive phase (ReP) in either year. High grazing significantly enhance carbon uptake, while light grazing reduce carbon uptake in ReP. Watering only delayed the browning time by 5.01 days in 2013, producing no significant effects on any phenophase. Watering promoted the net ecosystem exchange (NEE), ecosystem respiration (ER), and gross ecosystem productivity (GEP) only in the GrP. When calculating the yearly differences in phenophases and the corresponding carbon fluxes, we found that an extended GrP greatly enhanced NEE, but a prolonged ReP distinctly reduced it. The extended GrP also significantly promote GEP. Increases in growing season length appeared promoting ER, regardless of any phenophase. Additionally, the shifts in NEE appeared dependent of the variations in leaf area index (LAI).Chronic obstructive pulmonary disease (COPD) is currently the ninth leading cause of death in France and is predicted to become the third leading cause of worldwide morbidity and mortality by 2020. Risk factors for COPD include exposure to tobacco, dusts and chemicals, asthma and alpha-1 antitrypsin deficiency. This genetic disease, significantly under-diagnosed and under-recognized, affects 1 in 2500 live births and is an important cause of lung and, occasionally, liver disease. Alpha-1 antitrypsin deficiency is a pathology of proteostasis-mediated protein folding and trafficking pathways. To date, there are only palliative therapeutic approaches for the symptoms associated with this hereditary disorder. Therefore, a more detailed understanding is required of the folding and trafficking biology governing alpha-1 antitrypsin biogenesis and its response to drugs. Here, we review the cell biological, biochemical and biophysical properties of alpha-1 antitrypsin and its variants, and we suggest that alpha-1 antitrypsin deficiency is an example of cell autonomous and non-autonomous challenges to proteostasis. Finally, we review emerging strategies that may be used to enhance the proteostasis system and protect the lung from alpha-1 antitrypsin deficiency.Although the induction of neovascularization by cell-based approaches has demonstrated substantial potential in treating myocardial infarction (MI), the process of cell-mediated angiogenesis and its correlation with therapeutic mechanisms of cardiac repair remain elusive. In this work, three-dimensional (3D) aggregates of human umbilical vein endothelial cells (HUVECs) and cord-blood mesenchymal stem cells (cbMSCs) are constructed using a methylcellulose hydrogel system. By maximizing cell-cell and cell-ECM communications and establishing a hypoxic microenvironment in their inner cores, these cell aggregates are capable of forming widespread tubular networks together with the angiogenic marker αvβ3 integrin; they secret multiple pro-angiogenic, pro-survival, and mobilizing factors when grown on Matrigel. The aggregates of HUVECs/cbMSCs are exogenously engrafted into the peri-infarct zones of rats with MI via direct local injection. Multimodality noninvasive imaging techniques, including positron emission tomography, single photon emission computed tomography, and echocardiography, are employed to monitor serially the beneficial effects of cell therapy on angiogenesis, blood perfusion, and global/regional ventricular function, respectively. The myocardial perfusion is correlated with ventricular contractility, demonstrating that the recovery of blood perfusion helps to restore regional cardiac function, leading to the improvement in global ventricular performance. These experimental data reveal the efficacy of the exogenous transplantation of 3D cell aggregates after MI and elucidate the mechanism of cell-mediated therapeutic angiogenesis for cardiac repair.Systemic PDT (SPDT) approach is developed to treat a variety of hematological diseases, including cancers and blood-borne infections. We evaluated the efficacy of an SPDT method for treating leukemia using a Brown Norway myeloid leukemia (BNML) rat model with the LT12 cells engineered to express GFP. The survival times of animals receiving SPDT at 5 (early-SPDT) and 10 (mid-SPDT) days post-LT12 injection were prolonged by 2 days, the rats in the late-SPDT group (15 days) exhibited a 6-day increase in life span (p less then 0.05). The percentages of GFP-LT12 cells in the bone marrow of the late-SPDT rats decreased from 61.6% to 56.5% on day 17. Likewise, there was a decrease in the serum expression levels of IL-1β, IL-10, TNF-α, and IFN-γ in the late-SPDT rats (p less then 0.05). Our findings indicate that SPDT could be an effective method for the treatment of leukemia, and that antitumor immunity may play a key role in this process.Three centric diatoms, Thalassiosira pseudonana (diameter ~4 μm), Thalassiosira weissflogii (~11 μm), and Thalassiosira punctigera (~47 μm), were exposed to low and high levels of UV radiation. UV-induced inhibition on photosystem II was correlated with cell size under high light levels, though it was insignificant under low light levels (PAR less then 63 Wm(-2)). Lithium Chloride molecular weight The highest inhibition (~15%) was observed for the smallest species. Several mechanisms may explain the observed relationship between cell size and response to UV. All three species counteracted UV-related photosystem damage via protein synthesis within the chloroplast. Non-photochemical quenching (NPQ) was induced when that process was blocked with an inhibitor in T. pseudonana and T. weissflogii, but not T. punctigera, as neither radiation nor the inhibitor had a significant effect on NPQ in this species. Moreover, UV-induced inhibition for cells treated with lincomycin was highest for T. weissflogii, which was in accordance with the highest UV exposure within the cell. The intracellular UV distribution was not associated with cell size, indicating that the package effect was not the only determinant of cell-size dependent UV sensitivity in phytoplankton.A solid-phase extraction (SPE) procedure using C18 stationary phase was optimized for the preconcentration of 19 fluorinated derivatives of benzoic acid (FBA) mono-, di-, tri-, and tetrafluorosubstituted in the ring, trifluoromethylbenzoic acid and 3,5-bistrifluoromethyl benzoic acid from undiluted salt-rich (>20%) reservoir waters. Quantitative (>90%) retention/elution of 16 out of 19 analyte compounds was achieved allowing a fourfold preconcentration factor accompanied by the elimination of >99% of salt. For the three most polar compounds (2,6-dFBA, 2,3,6-tFBA, and 2,4,6-tFBA) the non-quantitative recoveries (>70%) were corrected by dedicated custom-synthesized deuterated internal standards. The FBAs were determined by HPLC - MS/MS revisited in terms of a choice of column, elution conditions and MS/MS signal acquisition parameters allowing the baseline separation and a gain in sensitivity. For a sample intake of 4 mL, detection limits for all the compounds in a reservoir water sample containing more than 20% salt were between 0.01 and 0.05 ng/mL which represents a gain of a factor of 10-20 in comparison with the state-of the art LC-MS/MS procedures for samples of similar complexity.Recently, interest in supercritical fluid chromatography (SFC) has increased due to its high throughput and the development of new system improving chromatographic performances. However, most papers dealt with fundamental studies and chiral applications and only few works described validation process of SFC method. Likewise, evaporative light scattering detection (ELSD) has been widely employed in liquid chromatography but only a few recent works presented its quantitative performances hyphenated with SFC apparatus. The present paper discusses about the quantitative performances of SFC-ELSD compared to HPLC-ELSD, for the determination of plasticizers (ATBC, DEHA, DEHT and TOTM) in PVC tubing used as medical devices. After the development of HPLC-ELSD, both methods were evaluated based on the total error approach using accuracy profile. The results show that HPLC-ELSD was more precise than SFC-ELSD but lower limits of quantitation were obtained by SFC. Hence, HPLC was validated in the ± 10% acceptance limits whereas SFC lacks of accuracy to quantify plasticizers.