Leegallegos4854
Phenotypically, over-expressed plants and calli were healthier while knock-out plants and calli showed retarded growth under salinity stress. The study provides new insight for the role of AtCNGC19 and AtCNGC20 under salt stress regulation in A. thaliana and will be helpful for improvement of crop plants for salt stress to combat food shortage and security.
Migraine is a chronic neurovascular condition characterized by recurring attacks of pulsating headaches. Genome-wide association studies (GWAS) identified many potential loci associated with migraine. To check the association of polymorphisms of
(rs2651899),
(rs11172113), and
(rs10166942) with migraine, the first time a case-control study was conducted in understudied Pakistani population.
The study included 127 migraine patients (21 in migraine with aura and 106 with migraine without aura group) and 120 healthy control subjects from different areas of Punjab, Pakistan. Blood samples were collected from all the participants, and DNA was isolated from the lymphocytes by the modified organic method. Sanger's sequencing was done for
rs2651899),
(rs11172113), and
(rs10166942) in all the samples to check the genotype. Logistic regression analysis was done using SPSS 20.0 to check the association of these SNPs with migraine susceptibility.
We found statistically significant differences betwee-ever replication report from Pakistan, PRDM16 (rs2651899) was found as a potential genetic marker in migraine susceptibility while LRP1 (rs11172113) and TRPM8 (rs10166942) showed partial association in subgroup analysis.Twenty five yeasts isolated were isolated from Khurais oil field in Saudi Arabia and assayed to evaluate their biodegradability. Only five isolates (namely, A1, A2, A3, A4 and A5) showed potential use of oil as sole carbon source. During incubation period, highest growth rate were recorded for A1, A2 and A3 isolates. Low growth distinguished A4 isolate; A5 isolate could not degrade oil. Spectrophotometrical analysis for four yeast isolates biodegradation activities indicated that, A1 isolate was superior for oil degradation (61%) comparing with A4 isolate which reflected lowest degradation % (33%). A2 and A3 isolates showed moderate biodegradation activity (56 and 51% respectively). D1/D2 domain of the 26S rRNA gene sequence was used as molecular marker to identify five yeast isolates. After comparing 26S rRNA gene sequences of five yeast isolates with highly similarity isolates, five yeast isolates (A1, A2, A3, A4 and A5)were submitted to database as Candida tropicalis (MW488263), Candida tropicalis (MW488264), Rhodotorula mucilaginosa (MW488265) and Rhodosporidium toruloides (MW488266) respectively. Using OXF1/ACR1 primer, specific lipase gene amplicon with 250 bp were detected with in all four yeast isolates.
Cognitive impairment is an aging-related disease that can result in a variety of health problems as disability and death. Cognitive impairment was reported to be more than 40% among elderly individuals.
To predict cognitive impairment among geriatric patients at Asir central hospital, Saudi Arabia and its relationship to health status.
A descriptive correlational study design was used to conduct this study. The study included a convenient sample of all geriatric patients (130) attending outpatient clinics of Asir central hospital in Abha city from the first of February to the mid of March 2020. Three tools were utilized to collect data pertinent to this study; Tool (I) structured geriatric patient's sociodemographic and clinical data interview questionnaire, Tool II Geriatric Depression Scale Short-Form (GDS-SF) and Tool III Mini Mental State Examination (MMSE) Scale.
The majority of the studied sample was in the age group between 60 and 69years, and female, having a chronic disease, (31.6%) were having a mild cognitive impairment and (17%) were having severe cognitive impairment and there was an association between cognitive impairment levels and health status of the studied patients with no statistically significant difference.
Nearly one third were having a mild cognitive impairment and about one fifth were having severe cognitive impairment. There was a correlation between levels of cognitive impairment and health status of the studied patients.
Health education programs to increase the awareness of the Saudi community about cognitive impairment and its risk factors are needed. Elderly cognitive screening services must be readily available for early diagnosis and early treatment of cognitive impairment.
Health education programs to increase the awareness of the Saudi community about cognitive impairment and its risk factors are needed. Elderly cognitive screening services must be readily available for early diagnosis and early treatment of cognitive impairment.The present study focused on extracting green larvicides from extracts of the combination of Foeniculum vulgare and Matricaria chamomilla using different solvents of increasing polarity in a Soxhlet extractor and evaluating their ovicidal, larvicidal, and cytotoxic activities. The most promising among all tested extracts was hexane extract. The ovicidal activity of the hexane PH2 extract resulted in a significant (p less then 0.05) decrease in egg hatchability from 95.00 ± 6.16% to 15 ± 9.04% at doses ranging from 62.5 to 500 µg/mL. The larval mortality with the hexane extract ranged from 13.33 ± 3.3% to 93.33 ± 3.3% at doses ranging from 31.25 to 250 µg/mL, respectively. The LC50 and LC90 values of the larvicidal activity of the hexane extract were estimated to be 148.3 and 242.17 µg/mL, respectively, after 24 h of exposure. Similarly, the LC50 values after 48 and 72 h of exposure were 124.93 and 100.3 µg/mL, respectively, against the third instar of Cx. pipiens. PH2 treatment of larvae resulted in histopathological changes such as degenerated epithelial cells and destruction of microvilli on the epithelial cells. The PH2 extract achieved a dose-dependent decrease in the rate of cell survival. The IC50 value of PH2-treated HUVECs was 192.07 µg/mL after 24 h of incubation. The cells showed changes in cellular and nuclear morphology. In conclusion, the hexane extract of PH2 could be used in mosquito management programs.This study aimed to develop a method for identifying different cultivars of Indian jujube fruits (Ziziphus mauritiana Lamk.) based on a single Indian jujube fruit color and morphological attributes using an artificial neural network (ANN) classifier. Eleven Indian jujube fruit cultivars were collected during winter of season 2020 from a local orchard located at Riyadh region, Saudi Arabia to measure their lengths, major diameters, and minor diameters. Different morphological descriptors were calculated, including the arithmetic mean diameter, the sphericity percent, and the surface area. Moreover, the color values of L*, a*, and b* of the skin of fruits were recorded. The ANN classifier was used to identify the appropriate class of Indian jujube fruit by using a combination of morphological and color descriptors. The proposed method achieved an overall identification rate of 98.39% and 97.56% in training and testing phases, respectively. In addition to color and morphological features, ANN classifier is a useful tool for identifying Indian jujube fruit cultivars and circumventing the difficulties met during fruit grading.
Most Hepatocellular Carcinomas (HCCs) are diagnosed at an advanced stage. However, HCC early diagnosis is complicated by the coexistence of inflammation and cirrhosis. The unsatisfactory sensitivity and specificity of Alpha-fetoprotien (AFP) for screening of early-stage HCC paved the way for new novel biomarkers to complement AFP such as AFP-L3. The aim of this study was the Evaluation of alpha fetoprotein-L3 (AFP-L3) as earlier marker in diagnosis of hepatocellular carcinoma in Egyptian patients. This study was conducted on 80 patients categorized into 2 groups; group 2 (40 patients with chronic active hepatitis) and group 3 (40 patients with HCC). HCC diagnosis was done byclinical, triphasic CT and positive US for focal lesion, in addition to 20 healthy individuals as controls (group 1).
The median range of AFP and AFP-L3 were highly statistically significant difference between HCC group and other groups [
<0.001]. In this study ALT, AST, Total & direct bilirubin and albumin results showed highly significant differences between HCC group and other groups. Serum AFP-L3 shows sensitivity 100%, specificity 100%, positive predictive value 100% and negative predictive value 100% with AUC=1 in HCC cases.
Serum AFP-L3 may serve as a diagnostic biomarker for the detection of early stage of HCC and show higher sensitivity than AFP.
Serum AFP-L3 may serve as a diagnostic biomarker for the detection of early stage of HCC and show higher sensitivity than AFP.Spodoptera frugiperda is a highly polyphagous migratory lepidopteran pest species. It causes infestation in crops leading to the severe crop losses. Being a new invasive parasite, its susceptibility to insecticides needs to be explored; and therefore, there is an urgent need to develop the potent insecticides for the effective control of this insect pest. To attain the crop sustainability, the antifeedant, toxicity and nutritional effects on larvae of Spodoptera frugiperda were studied with six mono- and eight bis- substituted chalcones. The antifeedant activity was calculated when 50% of the larvae control ate 50% of the diet through the FR factor. Toxicity was assessed through larval, pupal mortality and the emergence of adults and nutritional effects with consumption rates (IC), growth (GR) and consumption efficiency (EIC). The bis-chalcones 6b, 6e, 6f and 6h caused lethal effect on S. frugiperda in the first larval stages, being 6b the most toxic (85%). Adults who survived showed malformations and decreased size, which led to death. The larvae fed with aggregate in the bis-chalcones diet 6b, 6e and 6f had the highest percentage of intake and the poorest conversion of nutrient absorption (ECI), which suggests that the larva metabolizes food for energy and results in a decrease of growth and death in early stages. Bis-chalcones showed more toxicity than mono-chalcones and 6b causes the most toxic and dietary change.Seaweeds have been focused as potential and promising resources to develop novel pharmaceuticals. The present study was aimed to investigate the bioactive principles of Sargassum crassifolium (S. crassifolium) through organic solvents methanol and petroleum ether extractions individually. The present study also extended to determine the antibacterial potentiality of the bioactive principles from methanolic extract (ME) and petroleum ether extract (PEE) of S. Fatty Acid Synthase activator crassifolium against a set of human pathogenic bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT-IR) analysis of the ME and PEE were exhibiting unique bioactive constituents. The antibacterial effect of ME and PEE were showed the moderate spectrum of activity when compared to the standard streptomycin disc against the screened human pathogenic bacteria. The bacterial sensitivity to the ME was sequenced as Bacillus subtilis > Pseudomonas aeruginosa > Escherichia coli > Klebsiella pneumoniae > Staphylococcus aureus > Streptococcus pyogenes.
