Kragelundtrue4573

Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite.There are large social inequalities in health. The purpose of this study was to evaluate the effects of a family intervention on physical activity (PA) and sedentary time (ST) in children and their parents. In this controlled pilot study, all 8-9-year-old children from four schools from a socioeconomically disadvantaged area in Sweden were invited and 67 children and 94 parents were included. The intervention was run by a foundation in co-operation with the municipality. The 9-month program included (1) activity sessions, (2) healthy meals, (3) health information and (4) parental support groups. PA was primary outcome and ST was secondary outcome, measured by accelerometry. In total, 40 of the children (60%) and 45 of the adults (50%) had at least one day of valid accelerometer data at both baseline and follow-up. Significant intervention effects for the whole group were found in total PA (p = 0.048, mean difference (MD) intervention/control 150 counts per minute) and in vigorous PA (p = 0.02, MD 8 min/day) during the weekends. There were no differences between groups in the other PA variables or ST. This pilot study shows that it is possible to influence PA in families from a disadvantaged area through a family program.The noncommunicable diseases' (NCDs) profile is changing rapidly from one country to another. A well-formulated cohort study in Africa could answer major questions relating to the changing profile of NCDs risk in Africa. The aim of the present study was to outline the genesis, procedures, attrition rate and major reasons for study participants to miss measurement sessions in the Ellisras Longitudinal Study (ELS). Method The ELS followed multiple longitudinal designs comprising repeated measurements in more than one cohort with overlapping ages. Age cohort and time of measurement effects could be identified. A cluster random sampling method was used to sample 2255 participants (1201 males and 1054 females), aged 2 to 10.9 years at baseline (November 1996). Information on lifestyle (tobacco and smoking, alcohol intake, physical activity and socioeconomic status) and biological risk factors for NCD and educational achievements were collected over time. The participants were followed 17 times over the past 25 years with measurements (blood pressure and anthropometry) collected twice during the first consecutive 8 years to account for growth dynamics and other health-related variables. The attrition rate for ELS sample for boys (14%-27.3%) was significantly (p less then 0.05) higher than girls (7.9%-18.6%) from May 1999 to November 2003. There was a significant (p less then 0.05) increase (25.3%-70.3%) in attrition rate from November 2009 to December 2015. The ELS participant migration to urban areas provided a unique opportunity to investigate the effect of urban life on these rural young adults given the previous data collected on the same subjects at a younger age (3-10 years at baseline in 1996). Conclusion A well-formulated ELS study in Africa could answer major questions relating to the changing magnitude of NCDs risk factor profiles in Africa.Inflammation is often equated to the physiological response to injury or infection. Inflammatory responses defined by cytokine storms control cellular mechanisms that can either resolve quickly (i.e., acute inflammation) or remain prolonged and unabated (i.e., chronic inflammation). Perhaps less well-appreciated is the importance of inflammatory processes central to healthy pregnancy, including implantation, early stages of placentation, and parturition. Pregnancy juxtaposed with disease can lead to the perpetuation of aberrant inflammation that likely contributes to or potentiates maternal morbidity and poor fetal outcome. Maternal obesity, a prevalent condition within women of reproductive age, associates with increased risk of developing multiple pregnancy disorders. see more Importantly, chronic low-grade inflammation is thought to underlie the development of obesity-related obstetric and perinatal complications. While diverse subsets of uterine immune cells play central roles in initiating and maintaining healthy pregnancy, uterine leukocyte dysfunction as a result of maternal obesity may underpin the development of pregnancy disorders. In this review we discuss the current knowledge related to the impact of maternal obesity and obesity-associated inflammation on uterine immune cell function, utero-placental establishment, and pregnancy health.