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the analyses. Data were analyzed using the Statistical Package for Social Sciences (SPSS) software version 24. Cronbach's alpha showed for the group, Health and Consumer behavior in Contact lens wearers (6 items), to reach acceptable reliability α= 0,881. Cronbach's alpha showed for the group, Perceived risk of infection due to COVID-19 in contact lens wearers (6 items), to reach acceptable reliability α= 0,886. The data set can be used to understand the relationship between the perceived risk of Covid-19 infection and contact lens (CL) use, the potential effect of the pandemic in Health and consumer behavior in Contact lens users, to draft a survey strategy and design, to verify results, etc.In response to the current global challenge due to COVID-19, a dataset in this paper presented survey data of COVID-19 vaccine side effects among hospital staff in a national referral hospital in Indonesia. This survey data included the hospital staff of Dr. M. Djamil Hospital Padang, a national referral hospital in Indonesia, through a survey distributed via an online questionnaire, assessing COVID-19 vaccine side effects from 9th February to 13th February 2021. The items of the side effects included swelling, redness, itching, fever, headache, muscle pain, fatigue, coughing, diarrhea, nausea and vomiting, breathlessness, joint pain, fainted, anaphylactic reaction, itch, and swollen lymph nodes. In this survey data, we collected a total of 840 responses. The survey data were analyzed using univariate and bivariate analysis. Data analysis was performed using IBM version 25.0.

Large-animal models such as sheep for facial nerve regeneration research have not yet been established because of the lack of methods for assessing the electrophysiological function of regenerated nerves. In this study, we developed a percutaneous measurement method for the evoked compound muscle action potential (CMAP) of the facial nerve in sheep.

Six 3-year-old castrated male Corriedale sheep were used in this study. Under general anesthesia, an anatomical exploration was performed to identify the course of the buccal branch of the facial nerve and its innervating muscles on one side, followed by the application of surface stimulating electrodes to the contralateral side of the face along the course of the buccal branch of the facial nerve to obtain CMAP measurements of the nasolabial levator muscle.

Percutaneous CMAP measurements of the nasolabial levator muscle could be obtained in all animals by placing stimulating electrodes 1cm apart on the line coinciding with the course of the buccal branch of the facial nerve revealed by the preceding anatomical exploration. Mean values for electrophysiological parameters were amplitude 4.7±0.7mV, duration 2.1±0.6ms, and latency 3.6±0.4ms.

We have established a percutaneous measurement method for CMAP of the buccal branch of the facial nerve in sheep. This method is expected to be very useful in future studies of facial nerve regeneration for long nerve defects in sheep.

We have established a percutaneous measurement method for CMAP of the buccal branch of the facial nerve in sheep. This method is expected to be very useful in future studies of facial nerve regeneration for long nerve defects in sheep.Symptoms like bright yellowing, puckering of the leaf, vein banding, and vein thickening were observed on different cucurbit hosts at the experimental farm of Indian Agricultural Research Institute, New Delhi during Kharif 2019. Leaf-dip electron microscopy of the symptomatic leaves revealed the association of isometric virus particles measuring ~ 25 nm with bitter gourd and cucumber samples. The RT-PCR assay using polerovirus generic primers covering the partial RdRp, intergenic region, and partial CP region was resulted the amplicons of ~ 1.1 kb. Subsequent cloning, sequencing, and sequence analysis revealed the association of cucurbit aphid-borne yellows virus (CABYV) with bitter gourd (Momordica charantia) and cucumber (Cucumis sativus) plants. These results constitute the first report of CABYV infection on cucumber plants from India.

The online version of this article (10.1007/s13337-020-00645-4) contains supplementary material, which is available to authorized users.

The online version of this article (10.1007/s13337-020-00645-4) contains supplementary material, which is available to authorized users.For the first time, an isolate of the dichorhavirus orchid fleck virus (OFV, family Rhabdoviridae) was found infecting an orchid plant in Mexico. The infected sample of Epidendrum veroscriptum was collected in a nursery in Lagunillas, municipality of Zihuateutla, Edo. Puebla. Mites gathered on this plant were analyzed by light and scanning electron microscopy, which consistently indicated the presence of adults of the species Brevipalpus californicus, the common vector of OFV. Viral identification was based on symptoms, cytopathology, and reverse transcriptase-PCR/sequencing of genome fragments of the RNA1 and 2 molecules. Since isolates of OFV causing citrus leprosis have been previously detected in the Mexican states of Chiapas, Querétaro, and Jalisco, we promote a pertinent discussion and thought-provoking questions regarding the epidemiology and putative evolution of OFV.Iranian johnsongrass mosaic virus (IJMV, genus Potyvirus, family Potyviridae) is one of the most prevalent viruses causing maize mosaic disease in Iran. In this study, the complete genomes (9,618 and 9,543 nucleotides) of two highly divergent IJMV isolates (Maz2 and Maz3) were obtained from the metagenomic analysis of Zea mays RNAs using Illumina sequencing. The genome contained a single open reading frame (9,165 nucleotides) encoding a polyprotein of 3,054 amino acids, flanked by a 5'-untranslated region (UTR) of 216 and 143 nucleotides and a 3'-UTR of 237 and 235 nucleotides. A comparative analysis of the complete genome showed that IJMV-Maz2 and Maz3 had 85.99% nucleotide and 94.56% amino acid sequence identity with each other and shared 84.87-88.74% nt and 94.24-96.17% aa identity with those of two other IJMV isolates available in the GenBank. The coat protein of Maz2 and Maz3 showed 86.40-95.72% nt sequence identity (90.79-97.70% aa identity) to 12 other IJMV isolates available in GenBank. KRT-232 price Our results indicated a relatively stable and conserved genomic composition with a low codon usage bias in all of the assayed IJMV coding sequences.