Kayafrost9332

C-reactive protein, cystatin C, myoglobin, and D-dimer represent the inflammatory or thromboembolic status of the patient and play important roles in early diagnostics of acute myocardial infarction. Each protein can indicate some health problems, but their simultaneous detection can be crucial for differential diagnostics. The express analysis of these proteins in a small drop of plasma was developed using magnetic beads. The suggested method is based on immunomagnetic extraction of the target analyte from plasma samples and its simultaneous labelling by fluorescent dye. Reaction time was optimized for quantification of cardiac biomarkers in the spike solutions and human plasma samples. In this paper, we developed a one-protein detection technique for each cardiac biomarker and united it to a four-protein facility using an automatic platform. The proposed technique requires only 17 μL of the human plasma and takes 14 min for four-protein measuring. The suggested technique covers concentration difference by more than two orders of magnitude and demonstrates analytical applicability by measurements of human plasma samples of 16 volunteers.Herein we describe the design and synthesis of novel artificial peptides mimicking the plastoquinone binding niche of the D1 protein from the green photosynthetic alga Chlamydomonas reinhardtii, also able to bind herbicides. In particular, molecular dynamics (MD) simulations were performed to model in silico the behaviour of three peptides, D1Pep70-H, D1Pep70-S264K and D1Pep70-S268C, as genetic variants with different affinity towards the photosynthetic herbicide atrazine. Then the photosynthetic peptides were functionalised with quantum dots for the development of a hybrid optosensor for the detection of atrazine, one of the most employed herbicides for weed control in agriculture as well as considered as a putative endocrine disruptor case study. The excellent agreement between computational and experimental results self consistently shows resistance or super-sensitivity toward the atrazine target, with detection limits in the μg/L concentration range, meeting the requirements of E.U. legislation.Herein, we have synthesized Gd2O3Yb,Er@UiO-66-NH2 (UiO-66-NH2 represent a zirconium-based metal organic framework [Zr6O4(OH)4(ata)12], ata 2-aminoterephthalate) core-shell composites to develop an upconversion fluorescent nanoprobe for efficient detection of trace methylene blue (MB) and ferrous ions (Fe2+). Due to the fluorescence of the nanoprobe can be quenched by MB based on inner filter effect, but gradually recover when contact with ·OH, which is generated from the reaction between H2O2 and Fe2+, we have achieved the detection of Fe2+. The detection linear range is from 1.78 to 15.8 μM, and the limit of detection (LOD) is 0.071 μM. Besides, in this process, we also simultaneously realize the detection of MB. The linear range of MB turn-off detection is 0-42.6 μM, and the LOD is 0.41 μM. Doxycycline manufacturer To our knowledge, no example of using upconversion fluorescence probe for continuous detection of trace MB and Fe2+ has been reported for now, and test results are superior compared with most reported Fe2+ probes. Moreover, the combinations of upconversion nanoparticles (UCNPs) and the metal-organic frameworks (MOFs) have enhanced the selectivity and sensitivity of the probe towards MB and Fe2+. Therefore, we believe the designed upconversion fluorescent nanoprobe is a promising efficient tool in detecting MB and diagnose Fe2+ related diseases in the future.Reduced glutathione (GSH) and the oxidized glutathione (GSSG) are well-known biomolecules in the main constituents of intracellular redox homeostasis system. A rapid, accurate measurement of cellular GSH and GSSG is quite needed in investigating important biochemical events. In this work, we present a novel and sensitive method to monitor intracellular GSH and GSSG concentrations by a portable surface-enhanced Raman spectroscopy (SERS) technique. We introduced a reduction-sensitive reaction-type Raman probe, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to initiate GSH reduction, itself concomitantly converts to 2-nitro-5-thiobenzoic acid (TNB) to release a strong SERS signal. In a convenient way of inorganic salt MgSO4 induced aggregation of silver nanoparticles substrate, we easily implemented a good discrimination between DTNB and TNB, and a quantitative measurement of GSH and GSSG with a high sensitivity of 10 nM. This SERS method proved its feasible applicability in rapidly and sensitively monitoring GSH depletion behaviors of some notorious alkylating agents, i.e., sulfur mustard and nitrogen mustards in ex vitro or in vitro (cellular response). This SERS method may be very worthwhile in cellular detoxication event via the GSH approach and other GSH involved biomedical researches.In this paper, holey nitrogen-doped graphene aerogel (HNGA) was synthesized and applied to the concurrently electrochemical determination of small biological molecules including ascorbic acid (AA), dopamine (DA) and uric acid (UA). Firstly, holey graphene hydrogel was synthesized by the hydrothermal reaction in the presence of H2O2, which subsequently was lyophilized and further annealed in the mixed gas of ammonia and argon to obtain HNGA. Electron microscopy characterization exhibited a great number of nanopores formed on the basal surface of graphene sheets, and HNGA possessed a hierarchically porous structure. The unique structure and composition of HNGA make it an ideal material for electroanalytical application through accelerating mass and electron transfer. HNGA modified glassy carbon electrode (HNGA/GCE) displayed significantly enhanced electrochemical response to AA, DA, and UA, namely reducing overpotential, increasing current density, and improving the reversibility. The oxidation peaks of these three biomolecules can be entirely separated with evident peak potential differences which are 0.216 V (AA-DA), 0.120 V (DA-UA), and 0.336 V (AA-UA), which it allowed the determination of the three substances at the same time. This sensor shows high sensitivity for the determination of AA, DA, and UA with the detection limit of 16.7 μM, 0.22 μM, and 0.12 μM (S/N = 3), respectively. The proposed sensor was applicable for the practical sample analysis as well and desirable recovery was obtained.