Hickmantate2898

4% benign neoplasm (BN), 14.4% salivary gland neoplasm of uncertain malignant potential (SUMP), 1.9% suspicious for malignancy (SFM), and 5.8% malignant. Sensitivity, specificity, PPV, and NPV using MSRSGC were calculated as 75%, 98.4%, 88.9%, and 95.3%, respectively. RON/ROM for each category were 60%/15% for ND, 44.4%/0% for NN, 90%/40% for AUS, 100%/9.5% for BN, 100%/13.3% for SUMP, 50%/50% for SFM and 100%/100% for malignant. CONCLUSION The use of the Milan System proved to be a useful method to predict the risk of neoplasm and malignancy in the sample studied, with high sensitivity and specificity. © 2020 Wiley Periodicals, Inc.Johnson TM et al (1) deserve praise for their diligent systematic review and meta-analysis of conflicting reports on validity and performance of the multi-biomarker disease activity (MBDA) score for assessing rheumatoid arthritis (RA) disease activity. The MBDA is commercially available as Vectra DA; Crescendo Bioscience, South San Francisco, CA, USA. Using random-effects meta-analyses, the Johnson TM et al study (1) derived convergent validity of the MBDA score, i.e., its positive correlation with 4 other RA disease activity measures recommended in 2012 by the American College of Rheumatology (2). This article is protected by copyright. All rights reserved.Novel enamine derivatives (7a-o) were synthesized from the reaction of lactone (6) and chalcones (3a-o) and their antiproliferative and cell cytotoxicity activities against six cancer cell lines (e.g., HeLa, HT29, A549, MCF7, PC3 and Hep3B) and one normal cell line (e.g., FL) were investigated along with their mode of interactions with CT-DNA. Most of the enamine derivatives (7a-o) with 86-168 µM IC50 values demonstrated much stronger antiproliferative activity than the starting molecules (3a-o) against the cancer cells. While, among the enamine derivatives, the compounds 7e, 7f, 7k and 7l displayed higher cytotoxic potency than the control drugs (5-fluorouracil and cisplatin) against the Hep3B cell line, these compounds did not exhibit any significant toxicity against normal cells, FL. The UV-Vis spectral data suggest that the compounds 7c, 7e, 7i, 7l, 7m, 3d, 3i and 3o cause hypochromism with a slight bathochromic shift (~6 nm), indicating that they bind to the DNA by way of an intercalative or minor groove binding mode. The binding constants of the compounds are in the range of 0.1 × 103 M-1-2.3 × 104 M-1. The antiproliferative activity of studied enamine derivatives could possibly be due to their DNA binding as well as their cytotoxic properties. In addition to these assays, compounds 3a-3o and 7a-7o were investigated by molecular docking to calculate the synergistic effect of antiproliferative activities against six human cancer cell lines. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.The Middle East respiratory syndrome coronavirus (MERS-CoV) is an emergent respiratory virus. Dromedary camels are currently the only known reservoir of MERS-CoV and are capable of transmitting the virus within a herd. The role of semen in the transmission of MERS-CoV has never been investigated as yet, to the best of our knowledge. Our goal was to test semen collected from dromedary camels for MERS-CoV. A total of 67 seminal plasma samples from infertile and 13 from fertile dromedary camels was collected. The RNA was extracted from the samples and tested using commercial real-time PCR. Nine out of sixty-seven infertile animals (13.4%) were positive. The obtained PCR products were sequenced using the conserved MERS-CoV-N gene primers. MERS-CoV-RNA detected in seminal plasma was closely related to the lineage B. To the best of our knowledge, this is the first report about the detection of MERS-CoV-RNA in camel's seminal plasma. Regular testing of semen of common male camels' used for insemination should be considered to avoid a possible spread of the virus through semen. This article is protected by copyright. All rights reserved.BACKGROUND Lobular endocervical glandular hyperplasia (LEGH) is a disease considered to be the origin of tumorigenesis of minimal deviation adenocarcinoma, which has characteristic expression in the gastric pyloric mucosa. It is difficult to diagnose by nuclear findings because of lower nuclear atypia. ER stress inhibitor In this study, nuclei of endocervical (EC) and LEGH cells were digitized, and nuclear information was quantified from nuclear images and objectively evaluated using a computer. We examined whether it is possible to distinguish between EC and LEGH cells, which is difficult by human eyes. METHODS Signal intensity, morphological features, Otsu thresholding technique and gray-level co-occurrence matrix (GLCM) features were calculated from nuclei of EC and LEGH cells on cytology microscopic images. Then, discriminant analysis was performed using the significant difference test and linear support vector machine (LSVM). RESULTS GLCM features in LEGH cells were higher than those in EC cells. The nuclei of LEGH cells had a higher frequency of signal value pairs with a larger signal value difference than that of EC cells. Therefore, LEGH cell nuclei are thought to have more chromatin granules, and the chromatin is coarse and granular. Moreover, in the LSVM discriminant analysis, the accuracy of GLCM calculated using these features was 85.4%. CONCLUSION In this study, GLCM accurately demonstrated the nuclear chromatin distribution and coarseness. Discriminant analysis of EC and LEGH cells using GLCM features is useful. © 2020 Wiley Periodicals, Inc.OBJECTIVES We aimed to assess the in-hospital outcomes in patients with mitral regurgitation treated with percutaneous mitral valve repair (PMVR) among patients with chronic obstructive pulmonary disease (COPD). BACKGROUND There is lack of data on the outcomes of PMVR for mitral regurgitation in patients with COPD. METHODS We analyzed the national inpatient sample (NIS) database from January 2012 to December 2016. RESULTS A total of 9125 patients underwent PMVR in the period between January 2012 and December 2016, of whom 2,495 (27.3%) patients had concomitant COPD. Comparing COPD patients to non-COPD patients, COPD patients had higher proportion of females (48.3% vs. 46.6%, p = .16), were younger (75.8 ± 10.0 years vs. 76.4 ± 12.2 years; p = .04), had higher prevalence of peripheral vascular disease (17.4% vs. 13.5%; p  less then  .01) and renal failure (39.3% vs. 37%; p  less then  .01). After propensity matching, there was no significant difference in mortality among the COPD group versus non-COPD patients (2.6% vs. 2.9%; p = .6). Patients with COPD had higher proportion of in-hospital morbidities including St-segment elevation myocardial infarction (1.8% vs. 1.0%; p = .02), cardiogenic shock (1.4% vs. 0.4%; p  less then  .01), vascular complications (2% vs. 0.8; p  less then  .01), pneumothorax (1% vs. 0.4%; p  less then  .01), and septic shock (1.2% vs. 0.4%; p  less then  .01). Moreover, surrogates of severe disability (mechanical intubation and non-home discharges), cost of hospitalization, and length of stay were higher in the COPD group. CONCLUSIONS There was no difference in mortality between the COPD and non-COPD patients after PMVR. Moreover, we observed higher rates of in-hospital morbidities, surrogates of severe disability, and higher resources utilization by the COPD group. © 2020 Wiley Periodicals, Inc.BACKGROUND Outpatient telemedicine consultations are being adopted to triage patients for head and neck cancer. However, there is currently no established structure to frame this consultation. METHODS For suspected referrals with cancer, we adapted the Head and Neck Cancer Risk Calculator (HaNC-RC)-V.2, generated from 10 244 referrals with the following diagnostic efficacy metrics 85% sensitivity, 98.6% negative predictive value, and area under the curve of 0.89. For follow-up patients, a symptom inventory generated from 5123 follow-up consultations was used. A customized Excel Data Tool was created, trialed across professional groups and made freely available for download at www.entintegrate.co.uk/entuk2wwtt, alongside a user guide, protocol, and registration link for the project. Stakeholder support was obtained from national bodies. RESULTS No remote consultations were refused by patients. Preliminary data from 511 triaging episodes at 13 centers show that 77.1% of patients were discharged directly or have had their appointments deferred. DISCUSSION Significant reduction in footfall can be achieved using a structured triaging system. Further refinement of HaNC-RC-V.2 is feasible and the authors welcome international collaboration. © 2020 Wiley Periodicals LLC.The high reactive oxygen species (ROS) generation and simple construction of sonosensitizer systems remain challenging in sonodynamic therapy against the hypoxic tumor. In this work, we rationally prepared MOF-derived double-layer hollow manganese silicate nanoparticle (DHMS) with highly effective ROS yield under ultrasound irradiation for multimodal imaging-guided sonodynamic therapy (SDT). The presence of Mn element in DHMS increased ROS generation efficiency, because it could be oxidized by holes to improve the separation of electron-hole. Moreover, DHMS could produce oxygen in the tumor microenvironment, which helps overcome the hypoxia of the solid tumor and thus enhance the treatment efficiency. In vivo experiments demonstrated efficient tumor inhibition in DHMS-mediated SDT guided by ultrasound and magnetic resonance imaging. This work presented a MOF-derived nanoparticle harboring sonosensitive and oxygen generating ability, which provides a promising strategy for tumor hypoxia in SDT. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Compared to silica and alumina, graphene has received little attention as catalyst support, although it may represent a good candidate in view of its inertness, its good mechanical and thermal properties and its extended π conjugated system. While lanthanide catalysts grafted on silica have been synthetized and theoretically investigated, the grafting mode of La complexes onto graphene surfaces remains unknown. To shed light on this reaction, we report here a computational density functional theory study on the grafting of the [La(N(SiMe3)2)3] compound on graphene -OH and COOH functionalised surfaces. Similarly to experimental approaches, the Lewis acidity of these two supported complexes has been probed by coordination of triphenylphosphine oxide and compared with that of silica grafted analogues. In order to investigate the role of the graphene support in the catalytic activity of the corresponding grafted systems, we have then computed the homo- and co-polymerization reaction of ethylene and 1,3-butadiene. These compounds efficiently catalyse the ethylene and 1,3-butadiene homopolymerization, displaying activation barriers which are significantly lower than those reported for silica grafted compounds. The copolymerization reaction has been finally investigated. The results computed show that the high stability of the allylic products prevents the formation of alternating ethylene/butadiene copolymers, allowing the formation of block copolymers. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.