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arrying out single case studies.The spread of coronavirus disease 2019 (COVID-19) became a collective trauma adversely affecting physical and psychological health. The impact of this trauma made itself manifest in a myriad of ways, including through dreams. This study aimed to explore the Referential Process (RP, Bucci, 2021) of dreams reported during quarantine. Dream samples were collected through a social blog. Linguistic analysis and clinical evaluation were conducted to explore the group's collective elaboration of a shared traumatic experience. Sixty-eight people (22 males; mean age 26.16 ds. 7.68) contributed to a social-blog, writing their dreams. 91 dreams were collected and transcribed using transcription rules for the Discourse Attributes Analysis Program (DAAP). Linguistic measures of RP were applied and a statistical cluster analysis was performed. In addition, each dream was evaluated by trained judges on three specific qualities of the RP (Arousal, Symbolizing, and Reflection/Reorganizing). Clinical judges in a double-blinded method reached reliable scoring (Arousal α.874 ICC 0.701; Symbolizing α.783 ICC 0.671; Reflection/reorganization α.884 ICC 0.758). Cluster Analysis yielded three dream clusters. 26 dreams fell under a cluster defined as a symbolizing process (Cluster A); 16 into a cluster defined as arousal of emotional activation (Cluster B); and 49 dreams fell into a cluster defined as Reflection/Reorganizing elaboration (Cluster C). Each cluster showed specific linguistic features. The dreams collected through a blog showed a Referential Process that is similar to that found in psychotherapy process. Results suggests that writing dreams can play different functions in processing and integrating social traumatic experience.Based on the continuity hypothesis of dreaming, we have studied the effects of lockdown measures on Italian adolescents' and adults' dreams during the first wave of the coronavirus disease 2019 (COVID-19) pandemic. A sample of 475 subjects (73.9% women; 48.4% adolescents; ages 12-70 years, M=25.10, SD=12.2) was recruited via the Internet between April 17, 2020 and May 18, 2020. Participants were asked to provide socio-demographic data, as well as to report their Most Recent Dream. Results indicated that adults' dreams were the longest, as well as characterized by higher emotional intensity, predominantly negative emotions, and a higher presence of sensory impressions. Furthermore, results indicated that the participants, especially adolescents, who were most directly affected by the COVID-19 pandemic reported the strongest effects on their dreams. Results also indicated that women recall dreams more often than men, in addition to reporting higher emotional intensity, predominantly negative emotions, and a higher presence of sensory impressions. Finally, results of the Thematic Analysis of Elementary Contexts showed that adults' dreams seem to be centered, above all, on both pleasant memories linked to experiences that are now forbidden (i.e., traveling, meeting friends) as well as on nightmares, while adolescents' dreams focused on relationships with others. Finally, both adults and adolescents reported dreams related to the experience of home confinement, which they described in terms of the negative emotions they experienced. In sum, the findings of this study indicate that the COVID-19 lockdown measures, understood as a contextual and traumatic event, significantly affect people's oneiric lives, regardless of age.Nightmares are frightening or disturbing dreams that awaken sleepers while bad dreams are disturbing dreams that do not awaken sleepers. Both types are known to be associated with psychological symptoms including anxiety and depression. Chronic pain is often comorbid with psychological symptoms and vitamin D deficiency increases risk of chronic musculoskeletal pain (MSP), which in turn is associated with increased risk of anxiety and depression. We aimed to investigate associations between types of dreams, psychological symptoms, vitamin D, and calcium intake in individuals with MSP. The study included 191 outpatients with MSP and 191 age/gender matched healthy controls. Psychological symptoms were assessed using Hospital Anxiety and Depression Scale. Serum vitamin D was measured and daily calcium intake was estimated. Participants were asked about types of their dreams (normal, bad, or nightmares) during the past month. Binary logistic regression was used to find predictors of MSP and bad dreams and nightmares. Bad dreams and nightmares, vitamin D deficiency, low calcium intake, anxiety, and depression were more prevalent in cases versus controls (Ps less then 0.001). Chi-square analyses showed that types of dreams were associated with anxiety, depression, and MSP (Ps less then 0.001). Participants with normal dreams had higher vitamin D (P less then 0.01) and calcium intake (P less then 0.001) and lower anxiety and depression scores (Ps less then 0.001) compared to participants with bad dreams and nightmares. Anxiety, depression and MSP were predictors for bad dreams and nightmares. Further studies are required to assess if vitamin D supplementation and increasing calcium intake may improve MSP, psychological symptoms and thus prevent nightmares and bad dreams.Gestalt therapists believe that their task is to help their clients to experience repressed, ambivalent, and unpleasant things in order to accept and implement them in their whole self. To implement those 'things', those elements of the self, they need to be uncovered first, which is a process that often is achieved by dream work, as messages from the unconscious that are stuck in our dreams can be revealed by certain Gestalt-therapy methods. The method in focus is the newly developed DreamSenseMemory technique which is based on neurological findings on how the senses at play influence memory processing. Dream work with the DreamSenseMemory method has the advantage that by using this method on a regular basis, dream content will not only be remembered more often but also in more detail. Thus, effectively supporting dream work and its process of understanding the message of the unconscious, accepting the elements withing and implementing them in the self.[This corrects the article DOI 10.3389/fcimb.2021.704494.].Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that causes immunosuppression and neoplastic diseases in poultry. Cytokine signal-transduction inhibitor molecule 3 (SOCS3) is an important negative regulator of the JAK2/STAT3 signaling pathway and plays certain roles in ALV-J infection. It is of significance to confirm the roles of SOCS3 in ALV-J infection and study how this gene affects ALV-J infection. In this study, we assessed the expression of the SOCS3 gene in vivo and in vitro, and investigated the roles of SOCS3 in ALV-J infection using overexpressed or interfered assays with the SOCS3 in DF-1 cells. The results showed that the SOCS3 expression of ALV-J infected chickens was different from uninfected chickens in the spleen, thymus and cecal tonsil. Further, SOCS3 is mainly expressed in the nucleus as determined by immunofluorescence assay. Overexpression of SOCS3 in DF-1 cells promoted the replication of ALV-J virus, and the expression of interferons (IFNα and INFβ), inflammatory factors (IL-6 and TNFα) along with interferon-stimulating genes (CH25H, MX1, OASL, and ZAP). Conversely, interference of SOCS3 showed the opposite results. find more We also observed that SOCS3 promoted ALV-J virus replication by inhibiting JAK2/STAT3 phosphorylation. In conclusion, SOCS3 promotes ALV-J replication via inhibiting the phosphorylation of the JAK2/STAT3 signaling pathway. These results would advance further understanding of the persistent infection and the viral immune evasion of the ALV-J virus.Disseminated Leishmaniasis (DL) is an emerging and severe form of Leishmania (Viannia) braziliensis infection defined by the presence of 10 and up to more than 1,000 skin lesions. The mechanisms underlying parasite dissemination remain unknown. Genotypic differences among species of L. braziliensis have been associated with different clinical forms of disease. The present work compared the function of monocytes obtained from patients with cutaneous leishmaniasis (CL) and DL in response to infection with L. braziliensis isolates of both these two clinical forms of disease. Mononuclear cells obtained from DL and CL patients were infected with different L. braziliensis isolates, and numbers of infected cells, parasite load, respiratory burst, TLR2 and TLR4 expression and cytokine production were evaluated. DL isolates infected more monocytes, induced greater respiratory burst, and more cytokine production compared to isolates from CL patients regardless of the origin of monocytes (DL or CL). However, greater parasite multiplication and higher TLR2 and TLR4 expression were seen in monocytes from DL patients compared to CL following infection with DL isolates. Our results indicate the participation of both parasite genotype and host factors in the pathogenesis of DL.The involvement of the enteric nervous system, which is a source of S100B, in Clostridioides difficile (C. difficile) infection (CDI) is poorly understood although intestinal motility dysfunctions are known to occur following infection. Here, we investigated the role of S100B in CDI and examined the S100B signaling pathways activated in C. difficile toxin A (TcdA)- and B (TcdB)-induced enteric glial cell (EGC) inflammatory response. The expression of S100B was measured in colon tissues and fecal samples of patients with and without CDI, as well as in colon tissues from C. difficile-infected mice. To investigate the role of S100B signaling in IL-6 expression induced by TcdA and TcdB, rat EGCs were used. Increased S100B was found in colonic biopsies from patients with CDI and colon tissues from C. difficile-infected mice. Patients with CDI-promoted diarrhea exhibited higher levels of fecal S100B compared to non-CDI cases. Inhibition of S100B by pentamidine reduced the synthesis of IL-1β, IL-18, IL-6, GMCSF, TNF-α, IL-17, IL-23, and IL-2 and downregulated a variety of NFκB-related genes, increased the transcription (SOCS2 and Bcl-2) of protective mediators, reduced neutrophil recruitment, and ameliorated intestinal damage and diarrhea severity in mice. In EGCs, TcdA and TcdB upregulated S100B-mediated IL-6 expression via activation of RAGE/PI3K/NFκB. Thus, CDI appears to upregulate colonic S100B signaling in EGCs, which in turn augment inflammatory response. Inhibition of S100B activity attenuates the intestinal injury and diarrhea caused by C. difficile toxins. Our findings provide new insight into the role of S100B in CDI pathogenesis and opens novel avenues for therapeutic interventions.Cryptococcus neoformans is a fungal pathogen causing life-threatening meningoencephalitis in susceptible individuals. Fungal vaccine development has been hampered by the fact that cryptococcosis occurs during immunodeficiency. We previously reported that a C. neoformans mutant (Δsgl1) accumulating sterylglucosides (SGs) is avirulent and provides complete protection to WT challenge, even under CD4+ T cell depletion, an immunodeficient condition commonly associated with cryptococcosis. We found high levels of SGs in the lungs post-immunization with Δsgl1 that decreased upon fungal clearance. Th1 cytokines increased whereas Th2 cytokines concurrently decreased, coinciding with a large recruitment of leukocytes to the lungs. Depletion of B or CD8+ T cells did not affect either Δsgl1 clearance or protection from WT challenge. Although CD4+ T cell depletion affected clearance, mice were still protected indicating that clearance of the mutant was not necessary for host protection. Protection was lost only when both CD4+ and CD8+ T cells were depleted, highlighting a previously unexplored role of fungal-derived SGs as an immunoadjuvant for host protection against cryptococcosis.