Helboalstrup8500

Besides, grafted PNIPAM chains exhibited a higher glass transition temperature. The grafted keratin particles displayed enhanced hydrophilicity. Keratin-g-PNIPAMs exhibit a lower LCST comparing to homopolymer and the flocculation in hot water behavior could be controlled by regulating graft rate. V.As a highly efficient and environmentally friendly biocatalyst, immobilized lipase has received incredible interest among the biotechnology community for the production of biodiesel. Nanomaterials possess high enzyme loading, low mass transfer limitation, and good dispersibility, making them suitable biocatalytic supports for biodiesel production. In addition to traditional nanomaterials such as nano‑silicon, magnetic nanoparticles and nano metal particles, novel nanostructured forms such as nanoflowers, carbon nanotubes, nanofibers and metal-organic frameworks (MOFs) have also been studied for biodiesel production in the recent years. However, some problems still exist that need to be overcome in achieving large-scale biodiesel production using immobilized lipase on/in nanomaterials. This article mainly presents an overview of the current and state-of-the-art research on biodiesel production by immobilized lipases in/on nanomaterials. Various immobilization strategies of lipase on various advanced nanomaterial supports and its applications in biodiesel production are highlighted. Influential factors such as source of lipase, immobilization methods, feedstocks, and production process are also critically discussed. Finally, the current challenges and future directions in developing immobilized lipase-based biocatalytic systems for high-level production of biodiesel from waste resources are also recommended. V.Non-enzymatic degradation of wood via a chelator-mediated Fenton (CMF) system is the primary method for initial attack in brown rot fungal decomposition of wood, the most common type of fungal degradation of terrestrial carbon biomass on the planet. In this study, the degradation of thin films of cellulose and chitin by a CMF system was investigated and compared to enzymatic hydrolysis. The kinetics of the rapid cellulose and chitin deconstruction and the morphologies of the degraded cellulose and chitin surfaces were studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM), respectively. The QCM-D results quantitatively indicated that ~90 wt% of the regenerated cellulose or chitin was capable of being deconstructed by CMF action alone. While enzymatic degradation was consistent with stripping of layers from the surface of the cellulose or chitin films, the CMF process exhibited a pronounced two stage process with a rapid initial depolymerization throughout the films. The initial degradation rates for both model surfaces by the CMF system were faster than enzyme action. This research suggests that the CMF process should be applicable for the deconstruction of a wide variety of polysaccharides over Fenton chemistry alone. V.In this study, a novel pectate lyase (ApPel1) was identified and characterized from Aspergillus parasiticus. The ApPel1 hydrolysed oligogalacturonides (OGs) effectively and produced 4,5-unsaturated OGs from low-methoxyl (LM) pectin, with DP 2 to DP 5 as the major products. Furthermore, the multiple sequence alignments, structure model and phylogenetic analyses of the ApPel1 indicated that its catalytic active sites were highly conserved with other pectin lyases (PLs) and the Ca2+ binding amino acid residues are different compared with pectate lyases (Pels). N187D, N191D and N187D/N191D mutants were constructed to test for both Ca2+ binding properties and the effects on catalytic ability. The three mutations sharply decreased the activity of ApPel1 and Ca2+ tolerance, indicating that the Ca2+ binding amino acid residues are different from the other Pels. Based on the sequence and structure comparison between PLs and Pels, and mutation analysis, the ApPel1 may be direct evolution from PLs. Thus, this enzyme has potential for use in producing unsaturated OGs for biological activity study, and contributes to an improved understanding of the evolutionary relationships between PLs and Pels. A novel hybrid biocomposite based on amino-thiocarbamate derivative of alginate, carboxymethyl chitosan and TiO2 (TiO2/TSC-CMC) was fabricated and characterized using Fourier transform Infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDX). The TiO2/TSC-CMC mass ratio (5.0-30.0%) was optimized and 3TiO2/TSC-CMC (hydrogel beads with TiO2/TSC-CMC mass ratio of 20.0%) was selected as the best sorbent for effective biosorption of Ni(II). Batch sorption experiments were conducted, instantaneous and equilibrium sorption capacities were investigated as function of pH, sorbent dose, initial metal concentration, contact time and temperature. Kinetic data could be well explained through pseudo second order rate equation (PSORE) depicting that the rate determining step involves the transfer of electron density from sorbent functional sites to central metal ion. Langmuir model fitted well with isothermal sorption data and maximum monolayer sorption capacity (qm) was computed as 172 mg/g at pH 6.0 and temperature 298 K. The values of thermodynamic parameters such as standard enthalpy change (16.94 kJ/mol) and standard Gibbs energy change (-18.67, -19.48, -20.57, and -21.38 kJ/mol) and standard entropy change (0.12 kJ/mol·K) concluded that sorption process is endothermic, spontaneous and resulted with increase in randomness. Hence, 3TiO2/TSC-CMC was found efficient and reusable sorbent. V.BACKGROUND A high rate of regression in young women with cervical intraepithelial neoplasia grade 2 has been recorded. selleck kinase inhibitor However, there is little prospective data by which to evaluate management guidelines. OBJECTIVE This study evaluates the American Society for Colposcopy and Cervical Pathology recommendations for follow up of young women with cervical intraepithelial neoplasia 2 using data created by a large prospective multicentre study of observational management. STUDY DESIGN Participants were 616 women under 25 years with biopsy-diagnosed cervical intraepithelial neoplasia 2 following a referral to colposcopy for an abnormal smear with no previous high grade abnormality. The protocol included colposcopy, cytology, and colposcopically-directed biopsy at the initial visit and at 6 and 12 month follow-ups visits and these data were analysed. Histology from the corresponding cervical biopsy was treated as the reference diagnostic test. For young women with cervical intraepithelial neoplasia 2, we aimed to determine; (1) the ability of colposcopy to identify women with cervical intraepithelial neoplasia 3 or worse at 6 months and (2) the ability of a) colposcopy, b) cytology, and c) the combination of cytology and colposcopy to identify residual high grade abnormalities at 12 months.