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Based on a 3-year field experiment (2015-2017) with two treatments, annual whole-film mulching (PM) and uncovered (CK), we analyzed the relationship between soil temperature, moisture, and soil hydrothermal movement in semi-arid area. The results showed that freezing-thawing processes under both PM and CK were one-way freezing and two-way melting. Compared with CK, the freezing period in PM treatment was lagged, freezing rate was slowed down, freezing depth was 20 cm shallower, but melting rate was faster, and melting period was shortened by 6-7 days. In freezing period, soil temperature gradients of PM and CK were positive, with heat being transmitted toward top soil layer, and the conduction strength in PM treatment was greater than CK. During the melting period, soil temperature gradient of PM was also positive, with heat being transmitted toward upper soil layer, and that of CK was conversed. Soil water in PM treatment transported to upper soil layer during freezing-thawing period, but it appeared a "down-up-down" movement mode under CK in freezing period, "up-down" in thawing period. There was positively correlation between temperature and moisture gradient in the freezing period under both PM and CK treatments, with closer correlation in PM than CK. During melting period, soil temperature and moisture gradient was positively correlated in PM treatment with soil heat and moisture moved upward synchronously, while that in CK was negatively correlated with soil heat and moisture simultaneously moved to the lower layer soil. Driven by soil temperature and moisture gradient, soil temperature in 0-10 cm, 10-20 cm and 20-30 cm layers increased by 1.13-1.34 ℃, 0.96-1.24 ℃ and 0.89-1.32 ℃, while average soil water content increased by 3.4%-5.6%, 1.4%-2.2% and 6.7%-7.8%, respectively in PM treatment before sowing. Our results indicated that PM could provide water and heat protection for re-greening of winter crop and sowing, emergence and seedling of spring-sown crops in semi-arid areas.Effects of five different nitrogen application rates (i.e., N0, 0 g·kg-1; N1, 0.05 g·kg-1; N2, 0.1 g·kg-1; N3, 0.15 g·kg-1; N4, 0.2 g·kg-1) on the growth of seedlings of eight different quinoa cultivars were investigated in a pot experiment. The results showed that 1) Across different nitrogen application rates, cultivar GB22 and OY had the highest biomass, but cultivar B2 had the lowest value. The highest flower mass ratio, stem mass ratio, root mass ratio, and leaf mass ratio were found in cultivar B2, GB22, R1, and W23, respectively. 2) The rate of nitrogen application significantly affected seedling growth. Compared with the control (N0), the maximum net photosynthetic rate and biomass accumulation were significantly higher in the lower nitrogen applications (i.e., N1 and N2 treatments), but were lower in the higher nitrogen applications (i.e., N3 and N4 treatments). The significant interactions between cultivar and nitrogen application rate on plant biomass indicated that different quinoa cultivars responded differently to nitrogen rate. The optimum nitrogen application rate (Nopt) required for cultivar R1, MY11, GB22 and OY was 0.05 g·kg-1; while that of cultivar GB11, DB, and B2 was 0.1 g·kg-1; but for cultivar W23, Nopt was less than 0.05 g·kg-1. 3) The interactions between cultivar and nitrogen application rate significantly affected biomass allocation. Below the highest nitrogen rate used (i.e., less than 0.2 g·kg-1), the flower and leaf biomass allocation increased with the increasing nitrogen rates. 4) Across different cultivars and nitrogen application rates, plant biomass was positively correlated to the maximum net photosynthetic rate, plant height, ground diameter, and specific leaf area, respectively. selleck products These results provided valuable information for the nutrition management of different quinoa cultivars.To explore the effects of different intercropping species on growth, nutrition absorption, and fruit quality of oriental melon, we examined plant height, stem diameter, root activity, contents of mineral elements (N, P, K, Ca and Mg), and fruit quality and yield of oriental melon under the oriental melon monoculture (MM), intercropping of fennel/oriental melon (FM), tillered-onion/oriental melon (TM), or wormwood/oriental melon (WM). Results showed that plant height of intercropping treatments was significantly higher than that of the monoculture over time. The stem diameter of the FM and TM treatments was significantly higher than that of the MM treatment, while there was no significant difference between the WM and MM treatments. At the stretch tendril stage, fruit setting stage, and fruit expanding stage, root activity of FM treatment was significantly higher than that of MM and TM treatments. The mine-ral elements contents of oriental melon plants in three intercropping treatments were higher than that in MM treatment in different degrees, with the mine-ral elements contents of FM treatment being higher than that of TM and WM treatments. The single fruit weights of FM and TM treatments did not differ from the MM treatment. The fruit quality indices of FM treatment were not lower than MM treatment, while some fruit quality indices (including the contents of glucose, fructose, and sucrose) of WM and TM treatments were lower than that under MM treatment. In summary, fennel was a suitable species for intercropping with the oriental melon.To investigate the effects of H2S on mitochondrial functions under low temperature stress, we analyzed the effects of 0.05 mmol·L-1 NaHS and 15 μmmol·L-1 HT (hypotaurine and H2S scavenger) on mitochondria antioxidant enzyme activities and mitochondrial permeability transition pore, mitochondrial membrane fluidity, mitochondrial membrane potential, Cyt c/a ratio and H+-ATPase activity in sweet cherry stigma and ovary with sweet cherry variety Zaodaguo under -2 ℃ low temperature stress. The results showed that low temperature stress increased the concentrations of mitochondrial H2O2 and MDA, enhanced the mitochondrial membrane permeability, but decreased the mitochondrial membrane fluidity, membrane potential, Cyt c/a and H+-ATPase acti-vity. Application of NaHS at 0.05 mmol·L-1 could effectively reduce the concentrations of H2O2 and MDA, and keep higher activities of SOD, POD and CAT of mitochondrial for longer time. Furthermore, application of 0.05 mmol·L-1 NaHS could decrease mitochondrial membrane permeability while increase mitochondrial membrane fluidity, membrane potential, Cyt c/a and H+-ATPase activity in stigma and ovary under low temperature stress.

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