Haagensenhampton3129

lusal support. Dental personnel need to carefully examine dentition conditions to assess the risk of occlusal collapse.

Danggui-shaoyao-san (DSS), a representative formula of Traditional Chinese Medicine (TCM) for promoting blood circulation and diuresis (Huo-Xue-Li-Shui) therapy, has been used to clinically nephrotic syndrome (NS) and relieve nephrotic edema.

To explore the effects and mechanisms of DSS in improving sodium retention and to identify the bioactive compounds from DSS.

DSS prescriptions were disassembled into Yangxue-Huoxue (YXHX) and Jianpi-Lishui (JPLS). A nephrotic rat model was induced with puromycin aminonucleoside (PAN), and the effects on urinary sodium excretion, urinary plasmin(gen) content, and plasmin activity of DSS, YXHX, and JPLS extracts were assessed. The inhibitory effects on urokinase-type plasminogen activator (uPA) and plasmin activity of extracts were evaluated in vitro. Bio-affinity ultrafiltration and high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (BAU-UPLC-Q/TOF-MS) were used to rapidly screen and qualitatively analyze the uPA/plasmin affinity compg uPA-mediated plasmin generation and plasmin activity in the kidneys could be possible mechanisms for DSS, as indicated by the results in PAN-induced nephrotic rats. We conclude that PGG is a potential bioactive compound responsible for the effect of DSS on natriuresis.

Intervening in sodium retention by inhibiting uPA-mediated plasmin generation and plasmin activity in the kidneys could be possible mechanisms for DSS, as indicated by the results in PAN-induced nephrotic rats. We conclude that PGG is a potential bioactive compound responsible for the effect of DSS on natriuresis.

For thousands of years, garlic (Allium sativum Linnaeus) has been consumed in food and health by numerous civilizations. Cryptosporidium (C.) parvum is an apicomplexan parasite that causes a gastrointestinal disease, with the most common symptoms being watery diarrhea. Although several substances have been tried for its anti-cryptosporidial action, there is no effective treatment for Cryptosporidium disease, especially in immunocompromised individuals. The present study aimed firstly to characterize the bio-active compounds in Allium sativum L. and secondly to evaluate its efficacy as a therapy for cryptosporidiosis especially in immunocompromised mice.

This was accomplished by evaluating the parasitological and histopathological parameters in the experimentally infected immunocompetent and immunocompromised mice. Also, the cytokine profile during the experimental time was recorded through the measuring of T helper (h)1, Th2 and Th17cells cytokines. Immunosuppressed mice were given 0.25μg/g per day of dexarasite diseases.

Allium sativum L. displayed high efficacy as a potential therapeutic agent against Cryptosporidium, which supports its traditional usage in parasite diseases.Co-amorphous systems have been developed to address the solubility challenge of poorly water-soluble crystalline drugs. However, due to the thermodynamic instability of amorphous forms, amorphization may result in recrystallization during manufacturing, storage, or dissolution, which is one of the main challenges in the pharmaceutical development of amorphous systems. This could also be the case in some co-amorphous systems with only non-strong interactions between the drug and the co-former (such as hydrogen bond formation and π-π interactions). In this study, a small amount of polymer was added to the binary co-amorphous mixture carvedilol (CAR) - tryptophan (TRP) at a molar ratio of 11 and subjected to mechanical activation by ball milling to produce amorphous systems, in order to investigate the effect of co-formulated polymer on the physical properties (solubility, stability, etc.) of non-strongly interacting binary co-amorphous mixtures. After co-formulating polymer to the binary co-amorphous system, stronger interactions were found between CAR and polymer than between CAR and TRP in the ternary polymer containing co-amorphous systems. Compared to the corresponding binary co-amorphous systems, larger areas under the dissolution curves were achieved, indicating an improvement in dissolution behaviour due to a more gradual increase in dissolved drug concentration and a longer period of maintaining drug supersaturation. There was no negative effect of polymer addition on physical stability at room temperature under dry storage conditions for 6 months. Therefore, it is possible to design ternary co-amorphous drug delivery systems with optimized dissolution characteristics by adding a small amount of polymer into co-amorphous binary systems.Conventional dissolution testing methods may not be suitable for long-acting periodontal drug products due to the small volume, slow fluid flow rate, and environment in the periodontal pocket. The objective of this study was to evaluate a 3D-printed small volume flow-through dissolution chamber system (modified from a previous study) for biorelevant and dose-discriminating testing. Three periodontal drug products with different dosage forms were tested Atridox, Arestin, and PerioChip. Modifications were made to suit the specific characteristics of these dosage forms. No significant differences were observed between the % drug release profiles in vitro and in vivo except for Atridox. The differences observed with Atridox could be related to the exposing surface area of the drug product. Similar differences were observed from this effect in COMSOL model simulations. Overall, the drugs show reasonable in vitro-in vivo correlations (R2 ≥ 0.91) with linear regression slopes close to unity. For dose discrimination between 75% and full dosing, significant differences were observed in the drug release data at specific time points of the products (p ≤ 0.05). The present results suggest that a small volume dissolution chamber with slow flow rate could potentially provide biologically relevant and dose-discriminating evaluations for periodontal drug products.Thin-film freeze-drying (TFFD) is a rapid freezing and then drying technique used to prepare inhalable dry powders from the liquid form for applications such as drug delivery to the lungs. Herein we report the preparation of aerosolizable dry powders of monoclonal antibodies (mAbs) by TFFD. We first formulated an IgG antibody with lactose/leucine (6040, w/w) or trehalose/leucine (7525) and tested their aerosol performance. The IgG 1% (w/w) formulated with lactose/leucine (6040, w/w) in phosphate buffered saline (PBS) (IgG-1-LL-PBS) and processed by TFFD was found to produce the powder with desirable aerosol properties. We then replaced the IgG with anti-programmed cell death protein (anti-PD-1 mAb), a specific antibody, to prepare a dry powder (anti-PD1-1-LL-PBS), which performed similarly to the IgG-1-LL-PBS powder. The aerosol properties of the anti-PD1-1-LL-PBS dry powder were significantly better when TFFD was used to prepare the powder than when conventional shelf freeze-drying (shelf FD) was used. The The least amount of subvisible mAb aggregates. Finally, we showed that anti-TNF-α, another mAb, can also be converted to a dry powder with a similar composition by TFFD. We conclude that TFFD can be applied to produce stable, aerosolizable dry powders of mAbs for pulmonary delivery and that formulations must be optimized to maximize aerosol performance and minimize protein aggregation.The study demonstrated the fabrication of new poly(glycerol adipate) (PGA) nanoparticles decorated with folic acid (FOL-PGA) and triphenylphosphonium (TPP-PGA) and the potential on the delivery of acetogenin-enriched Annona muricata Linn leaf extract to ovarian cancer cells. FOL-PGA and TPP-PGA were successfully synthesized and used to fabricate FOL-decorated nanoparticles (FOL-NPs) and FOL-/TPP- decorated nanoparticles (FOL/TPP-NPs) by blending two polymers at a mass ratio of 11. All nanoparticles had small size of around 100 nm, narrow size distribution and high negative surface charge about -30 mV. The stable FOL/TPP-NPs showed highest drug loading of 14.9 ± 1.9% at 15 ratio of extract to polymer and reached to 35.8 ± 2.1% at higher ratio. Both nanoparticles released the extract in a biphasic sustained release manner over 5 days. The toxicity of the extract to SKOV3 cells was potentiated by FOL-NPs and FOL/TPP-NPs by 2.0 - 2.6 fold through induction of cell apoptosis. FOL/TPP-NPs showed lower IC50 and higher cellular uptake as compared to FOL-NPs. FOL-NPs exhibited folate receptor-mediated endocytosis. FOL/TPP-NPs provided more advantages than FOL-NPs in terms of stability in physiological fluid, uptake efficiency and targeting ability to mitochondria and showed a promising potential PGA platform for targeted delivery of herbal cytotoxic extracts.The indiscriminate accumulation of plastic waste has prompted research that leads to obtaining biobased materials. this website The research aim was to evaluate the effect of incorporating fique bagasse microparticles (FBM) in a cassava starch-based foamed material. First, the FBM extraction conditions were established by acid hydrolysis, for which the effect of acid concentration (5, 10 and 15% H2SO4), temperature (70, 80 and 90 °C) and extraction time (3, 5 and 7 h) on particle size, functional groups, color, and thermal properties was evaluated. The addition of FBM to the foamed material was then carried out. To do this, a completely randomized design with five treatments (0, 0.5, 0.75, 1.0 and 1.25% FBM) was evaluated. The response variables were the apparent density, expansion and spring index, compressibility, water absorption, thermal properties and FTIR. The results showed that the acid concentration, temperature and time had an effect on the morphological, chemical and thermal properties of FBM, with 10%, 70 °C and 7 h being the conditions that allowed obtaining the smallest particle size (61.69 ± 12.88 μm2). Moreover, the FBM concentration had a significant effect on the physical and mechanical properties of the foam, unleashing the treatment properties of 0.75%. This indicates that FBM have potential for use in obtaining biobased materials.In this article we report a novel Ag NPs fabricated chitosan-agarose composite functionalized core-shell type Fe3O4 nanoparticle (Ag/CS-Agar@Fe3O4). The biogenic material was analyzed over a number of physicochemical methods like, FT-IR, FE-SEM, TEM, EDX, XRD, VSM and ICP-OES. In catalytic exploration we aimed the synthesis of diverse 2H-indazolo0-b]phthalazine-trione derivatives via one-pot three component coupling of phathalalhydrazide, dimedone and different aldehydes. It afforded good to excellent yields under solvent-less conditions. Robustness of the catalyst was justified by catalyst recyclability for consecutive 10 times, hot filtration and leaching tests. Again, biological activity of the material was evaluated by studying the antioxidant and cytotoxicity properties over lung and liver cancer cell lines. Antioxidant potential of Ag/CS-Agar@Fe3O4 was assessed by DPPH radical scavenging studies and the corresponding IC50 was found to be 96.57 μg/mL. Liver and lung cancer studies over Ag/CS-Agar@Fe3O4 was carried out by MTT assay against HepG2 and A549 cell lines. The corresponding IC50 values were found as 192.35 and 365.28 μg/mL respectively. % Cell viability of the nanomaterial decreased dose dependently over both the cell lines without any cytotoxicity on normal cell line. The results demonstrates Ag/CS-Agar@Fe3O4 nanocomposite to be an efficient chemotherapeutic drug against the lung and hepatocellular carcinoma cells.