Guerranygaard1477

TBE patients with the highest peak values of serum pNF-H, exceeding 10 000 pg ml-1, had a very severe disease course, with coma or tetraplegia. Patients requiring intensive care had significantly higher serum pNF-H levels than other TBE patients (P less then 0.01). Elevated serum pNF-H values were also observed in patients with incomplete recovery (P less then 0.05). Peak serum pNF-H levels correlated positively with the duration of hospitalization (P=0.005). Measurement of pNF-H levels in TBE patients might be useful for assessing disease severity and determining prognosis.Inherited retinal dystrophies (IRDs) are a heterogeneous group of diseases that affect more than 2 million people worldwide. Gene therapy (GT) has emerged as an exciting treatment modality with the potential to provide long-term benefit to patients. Today, gene addition is the most straightforward GT for autosomal recessive IRDs. However, there are three scenarios where this approach falls short. First, in autosomal dominant diseases caused by gain-of-function or dominant-negative mutations, the toxic mutated protein needs to be silenced. Second, a number of IRD genes exceed the limited carrying capacity of adeno-associated virus vectors. Third, there are still about 30% of patients with unknown mutations. In the first two contexts, precise editing tools, such as CRISPR-Cas9, base editors, or prime editors, are emerging as potential GT solutions for the treatment of IRDs. Here, we review gene editing tools based on CRISPR-Cas9 technology that have been used in vivo and the recent first-in-human application of CRISPR-Cas9 in an IRD.The Fanconi anemia (FA) DNA repair pathway is required for DNA inter-strand crosslink (ICL) repair. Besides its role in ICL repair, FA proteins play a central role in stabilizing stalled replication forks, thereby ensuring genome integrity. We previously demonstrated that depletion of replication protein A (RPA) induces the activation of FA pathway leading to FANCD2 monoubiquitination and FANCD2 foci formation. Thus, we speculated that FA-deficient cells would be more sensitive to RPA inhibition compared to FA-proficient cells. Following treatment with RPA inhibitor HAMNO, we observed significant induction in FANCD2 monoubiquitination and foci formation as observed in RPA depletion. In addition, HAMNO treatment caused increased levels of γ-H2AX and S-phase accumulation in FA-deficient cells. Importantly, FA-deficient cells showed more increased sensitivity to HAMNO than FA-proficient cells. Moreover, in combination with cisplatin, HAMNO further enhanced the cytotoxicity of cisplatin in FA-deficient cells, while being less toxic against FA-proficient cells. This result suggests that RPA inhibition might be a potential therapeutic candidate for the treatment of FA pathway-deficient tumors.Eleven Gram-negative, curved and S-shaped, oxidase activity positive, catalase activity negative bacterial isolates recovered from faeces of Anatolian ground squirrel (Spermophilus xanthoprymnus) in the city of Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR revealed that these isolates belonged to the genus Helicobacter. The 16S rRNA gene sequence analysis revealed that the 11 isolates had over 99 % sequence identity with each other and were most closely related to Helicobacter ganmani CMRI H02T with 97.0-97.1 % identity levels and they formed a novel phylogenetic line within the genus Helicobacter. Faydin-H64 and Faydin-H70T strains were subjected to gyrA and atpA gene and whole genome sequence analyses. These two Helicobacter strains formed separate phylogenetic clades, divergent from other known Helicobacter species. The DNA G+C content and genome size of the strain Faydin-H70T were 35.3 mol% and 1.7 Mb, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Faydin-H70T and its close phylogenetic neighbour H. winghamensis ATCC BAA-430T were determined as 81.7 and 34.9 %, respectively. Pairwise sequence comparison showed that it was closely related to H. ganmani CMRI H02T however it shared the highest ANI and dDDH values with H. winghamensis ATCC BAA-430T. The data obtained from the polyphasic taxonomy approach, including phenotypic characterization and whole-genome sequences, revealed that these strains represent a novel species within the genus Helicobacter, for which the name Helicobacter turcicus sp. nov., is proposed with Faydin-H70T as the type strain (=DSM 112556T=LMG 32335T).Electronic delocalization is essential to the properties of π-conjugated molecules. We introduce the inter-fragment delocalization index (IFDI) as an easy-to-use computational method for quantifying the electronic delocalization in π-conjugated oligomers and molecular wire models. We show that the IFDI is related to the torsion barriers of π-conjugated dimers, and to the single-molecule conductance of several π-conjugated fragments. The IFDI is a useful screening technique for comparing different π-conjugated subunits as components in organic electronics, since it can quantify the influence of substitution position, structure, and (anti)aromaticity on delocalization.A cross-sectional study was conducted in January 2020 to evaluate type 1 diabetes (T1D) management during and between incarcerations at the Los Angeles County Jail. Inclusion criteria were men with a T1D diagnosis who had been incarcerated twice within 5 years with at least two hemoglobin A1c values during each incarceration and a minimum of 3 months in the community between incarcerations. The primary outcome was the percentage change in A1c after release compared with the A1c during incarcerations. Five patients met inclusion criteria. During the first and second incarcerations, mean A1c was reduced by 13% and 9.6%, respectively. While in the community, mean A1c increased by 14%. These results highlight the importance of comprehensive reentry programs for patients with T1D, including linkage to community resources for diabetes management, so health gains made during incarceration are not lost.Electroconvulsive therapy (ECT) has a reputation for rapid alleviation of symptoms, mainly in depressive disorders. In the current literature little attention has been paid to speed of response of psychotic symptoms in schizophrenia. We present the case of a 67-year old woman, diagnosed with schizophrenia and presenting with clozapine-resistant psychotic symptoms that swiftly remitted with ECT. We review the literature on rapid remission of schizophrenic psychosis during a treatment with ECT.The combination of an eating disorder and diabetes mellitus type 1 is a serious double diagnosis. Compensation behavior is often present in the form of insulin restriction which can have debilitating health consequences. Studies on prevalence, diagnosis and mortality are well documented but literature regarding evidence-based treatments is limited. In this article we describe a case study of a patient with diabetes who was admitted to our treatment unit for eating disorders due to manipulation of her diabetes medication. On the basis of this case and a literature study, we conclude that the standard treatment for eating disorders is insufficient for this double diagnosis. An adapted treatment, aimed at both the eating disorder and the diabetes is necessary to increase the chance of recovery.

A psychological autopsy study (Mérelle e.a. 2020) demonstrates a subgroup of female adolescents with chronic suicidal behavior and severe internalizing problems.

To describe characteristics of the suicidal process and the challenges experienced in providing mental health care for this subgroup.

A case description and review of literature.

The persistent suicidal threat and the following despair of the patient and its parents are forcing care providers into an impasse the primary focus of treatment is to guarantee the patient's safety, whereby the treatment of underlying problems is underexposed. selleck inhibitor Based on expert knowledge we make recommendations including autonomy-promoting treatment policy, treating suicidality as a transdiagnostic phenomenon, creating a multidisciplinary network of care providers and making chronic suicidality tolerable for care providers.

We propose preliminary practical recommendations in our quest for optimal mental health care for chronic suicidal adolescents.

We propose preliminary practical recommendations in our quest for optimal mental health care for chronic suicidal adolescents.

Interpersonal behavioral style (IPBS) refers to the way in which someone behaves in social contact. IPBS affects the type and quality of different social interactions including the therapeutic relationship. The therapeutic relationship correlates with treatment outcome, IPBS possibly too. The direction of that relationship remains unclear.

To examine the predictive value of IPBS on treatment outcome in group treatment.

149 patients were divided in different types of IPBS degree of affiliation (kindness vs. hostility), degree of dominance (dominant vs. submissive), quadrants (combination of affiliation and dominance) and vector length (strength of IPBS). Treatment outcome was measured in experienced psychopathology, social anxiety and frequency of social contact. Groups were compared by using one-way analysis of variance (ANOVA). Significant differences were analyzed using Tukey's post-hoc analysis.

Vector length predicted all treatment outcome measures. The degree of dominance predicted only scores on social anxiety and frequency of social contact. The degree of affiliation did not predict any treatment results.

Findings suggest that adult patients with stronger IPBS and a more submissive, sub-assertive IPBS experienced lower social anxiety scores and increased frequency of social contact after treatment. A stronger IPBS also predicts lower experienced psychopathology post-treatment. The degree of kindness/hostility does not affect treatment outcome.

Findings suggest that adult patients with stronger IPBS and a more submissive, sub-assertive IPBS experienced lower social anxiety scores and increased frequency of social contact after treatment. A stronger IPBS also predicts lower experienced psychopathology post-treatment. The degree of kindness/hostility does not affect treatment outcome.

In January 2020 the Compulsory Mental Healthcare Act (Dutch Wvggz) was implemented. The Wvggz details the rights of patients with mental illness who require compulsory care. The law aims, amongst others, to improve the legal rights of patients and those close to them, for example by enabling the possibility to draw up their own action plan (AP) or care card.

To explore what health care professionals think of the possibilities for involvement by patients and those close to them, enabled by the Wvggz.

A qualitative study in which health care professionals were interviewed about the possibilities for involvement by patients and those close to them. We used thematic analysis to study the data from the interviews.

Health care professionals were positive about the idea to involve patients and those close to them, though they indicated that patients and those close to them were already involved before the law came into effect. The main difference was that their involvement was more documented, for instance patients can write their own AP or fill out a care card.