Goodwinmccleary3635
To compare the effectiveness of treatment with autologous activated platelet-rich plasma (PRP), administered to either the subendometrium (SE-PRP) or endometrial surface (intrauterine; IU-PRP), against controls.
Prospective observational cohort study.
Tertiary fertility unit.
Women aged <40 years with a history of recurrent implantation failure undergoing frozen embryo transfer (FET) (n = 318).
In SE-PRP, PRP was injected into the subendometrial space transvaginally in the luteal phase of the previous cycle of embryo transfer under ultrasound guidance (n = 55). In IU-PRP, PRP was administered during the index FET cycle when the endometrium was approximately 7 mm (n = 109). Both SE-PRP and IU-PRP groups were administered 300 μg of granulocyte colony-stimulating factor (G-CSF) subcutaneously once a day for 3 days to boost white blood cells (WBC) and growth factor production in the PRP sample. The control group consisted of women who did not choose PRP treatment and underwent standard FET with no inatment appears to improve FET outcome with an increase in OPR/LBR. However, SE-PRP treatment does not offer any advantage over lesser invasive IU-PRP treatment.
To develop a test for evaluating the annexin A5 M2 haplotype in invitro fertilization patients and preimplantation embryos.
Test performance was measured by comparing Sanger sequencing of parental blood DNA and quantitative real-time polymerase chain reaction (qPCR) of saliva DNA, 3 fibroblast cell line 7-cell aliquots and their corresponding purified DNA, 123 trophectoderm biopsy samples, and DNA isolated from 1 embryonic stem cell line along with the Mendelian inheritance expectations, embryo Sanger sequencing, and single-nucleotide polymorphism (SNP) microarray-based linkage analysis.
Preimplantation genetic testing laboratory research on IVF patient and embryo DNA.
An assay was developed for the detection of the M2 haplotype on saliva samples of 6 invitro fertilization patients. In addition, 13 patients who underwent preimplantation genetic testing with data on parental and embryo biopsy DNA available for research use were evaluated.
None.
The concordance rates between Sanger sequencing, SNP ar weight heparin, through selection of embryos without the M2 haplotype.
This study resulted in the development of a convenient saliva collection method and qPCR-based genotyping method to screen for the M2 haplotype. In addition, a novel method for testing preimplantation embryos has been established, providing an alternative to the use of low molecular weight heparin, through selection of embryos without the M2 haplotype.
To assess the impact of shipment and storage of sperm, oocytes, and blastocysts in vapor phase nitrogen compared with static storage in liquid phase nitrogen.
Prospective cohort-matched study.
Multiple invitro fertilization laboratories in an invitro fertilization network.
Fifty-eight human embryos, 32 human oocytes, 15 units of bovine semen.
Vapor vs. liquid nitrogen.
The postwarming survival of oocytes, sperm, and blastocysts, and the developmental potential of blastocysts during invitro extended culture.
Custom-designed labware, for use with the TMRW platform, enables continuous temperature monitoring during shipment and/or storage in the vapor phase robotic storage system. BTK inhibitor The highest temperature recorded for specimens shipped to a domestic laboratory was -180.2 °C with a mean ± SD of -190.4 ± 0.5 °C during shipment and -181.1 ± 0.6 °C during storage. Likewise, specimens shipped internationally had a high of -180.2 °C with a mean ± SD of -193.5 ± 0.6 °C during shipment and -181.2 ± 0.7 °C du the vapor phase nitrogen TMRW platform when compared with static liquid phase nitrogen storage. Our results suggest that the vapor phase cryostorage platform is a safe system to handle and store reproductive specimens for human assisted reproductive technology.
To study the effects of ovulation induction on mouse postnatal health, with a focus on growth pattern and glucose tolerance. To study the effect of ovulation induction on DNA methylation, we took advantage of the agouti viable yellow (A
) mouse.
Animal study.
University Setting.
Agouti viable yellow (A
)mice on a C57BL/6 background.
A
female mice were either allowed to mate spontaneously (control group, C) or after superovulation with 5 IU of PMSG and hCG (ovulation induction group, OI).
Birth parameters and postnatal growth of the offspring were followed up to 29 weeks of age. Body composition analysis was performed by EchoMRI; fasting insulin, intraperitoneal glucose tolerance tests, and glucose-stimulated insulin secretion by beta cells were assessed to study glucose metabolism.
Mice born to superovulated dams had lower survival rates, shorter anogenital distances, and shorter crown-rump lengths. Female mice generated by OI weighed less at birth, whereas male mice generated by OI had lower weight gain and had reduced lean mass. Glucose parameters, including islet functions, did not differ between the groups. No difference in agouti coat color was noted between the groups.
Ovulation induction resulted in mice having increased morphometric differences at birth and male mice showing reduced weight gain but no difference in glucose tolerance or agouti coat color.
Ovulation induction resulted in mice having increased morphometric differences at birth and male mice showing reduced weight gain but no difference in glucose tolerance or agouti coat color.
To measure the influence of exogenous insulin-like growth factor 1 (IGF1) on follicle growth and maturation in human ovarian cortical xenografts.
Xenotransplantation model.
University-based research laboratory.
Ovarian tissue was donated with consent and institutional review board approval by brain-dead organ donors or patients undergoing ovarian tissue cryopreservation for fertility preservation. Cortical fragments were transplanted into immunocompromised mice.
Cryopreserved ovarian cortical fragments from four women (aged 19, 25, 33, and 46 years) were transplanted into the gluteus muscle of immunocompromised mice in a fibrin matrix containing endothelial cells that were transduced with lentiviral particles encoding secreted IGF1. Xenografts were recovered after 3, 8, and 14 weeks. In addition, C57/Bl6 mice underwent intraovarian injection of saline or recombinant IGF1 (60 μg), followed by superovulation, analysis of ethynyl-deoxyuridine incorporation, and ribonucleic acid sequencing of the whole ults in near absence of antral stage follicles in long-term (14 weeks) xenografts. In mice, acute administration of IGF1 promotes follicle advance and increased oocyte yield. The results suggest that while superphysiological IGF1 alone advances the pace of growth among early/preantral follicles, a sustained and/or later-stage influence undermines antral follicle growth/survival or promotes premature luteinization. These findings provide a temporal framework for interpreting follicle growth/mobilization and may be useful in understanding the clinical application of human growth hormone in the context of assisted reproduction.
In this cost-of-illness study, we analysed the socio-economic burden of bladder cancer survivorship for the ten years after initial treatment in Germany during 2000, 2010 and 2020.
Bladder cancer follow-up guidelines were extracted from the European Association of Urology. Per patient costs were estimated with a micro-costing approach considering direct and indirect medical expenses derived from literature and official scales of tariffs. Three perspectives covering costs for patients, providers, and insurers were included to estimate societal costs.
Mean 10-year follow-up costs per patient amounted to EUR 2214 for low-risk, EUR 4758 for medium-risk, and EUR 11,325 for high-risk non-muscle invasive bladder cancer (NMIBC) in 2020. The mean economic burden of muscle-invasive and metastatic bladder cancer (MIBC) was EUR 8994 per patient. Overall expenditure rose by 65% from 2000 to 2020 across all cancer stages (p < 0.001). While insurers covered 38% of costs in 2000, only 31% of costs were reimbursed ines covered by insurance funds are necessary to care for cancer survivors.
Oral cancer (OC) is one of the global diseases that is the eighth rank among different others. Early intervention therapy had a positive impact on increasing the prognosis and the survival rate up to 5 years. The aim of the current study was to evaluate the knowledge, attitude, and practice of dentists about OC in the Democratic Republic of the Congo (DRC).
It was a cross-sectional analytical survey conducted in the primary oral health care centers in DR. Congo from February to April 2021. Only dentists working at any of the primary oral health care centers, public or private was included. Data were collected by using a survey of a 33-item questionnaire. The practice level, knowledge, risk factors, diagnosis for OC, attitude, and opinions of the dentists were recorded and evaluated. The SPSS 20 was used for data analysis and P<0.05 was considered significant.
Out of 162 dentists recorded, 65.4% were male and 34.6% were females. Half of the dentists (50.6%) had more than 5-15 years of practical experind training programs for dentists would serve to address the knowledge deficits and practice shortcomings about oral cancer screening, prevention, and early detection.
To systematically appraise the content within oncology podcasts and the individuals who speak on them.
A cross-sectional study.
We obtained a list of 33 current podcasts with substantial oncologic content through queries of predetermined search terms on the Apple Podcast Platform.
98 oncology-related podcast episodes.
The perceived attitude of the episode with predetermined categories including "Neutral," "Favorable," or "Negative," the number of conflicts of interest verbally disclosed by individuals featured on oncology podcasts, and the prevalence of general payments among featured physicians.
Among 33 oncology podcasts, the median number of episodes was 81 (IQR 25-129). Ninety-seven percent (n=32/33) of the podcasts included guests. The median episode run time was 2650min (IQR 1800 - 4175). Among the 98 episodes assessed, 47% ofepisodes (n=46/98) mentioned oncologic drugs, of which 57% (n=26) had a neutral disposition, 37% (n=17) had a favorable disposition and 7% (n=3) were negative. Across 98 episodes, we identified 194 featured individuals, of which 65% (n=126) had a medical degree (MD), and 85% (n=107/126) of these physicians received at least one general payment. Further, 83% (n=105/126) of physicians did not disclose payments.
Withinoncology-related podcasts, the majority of conversations about oncologic drugs are perceived as either favorable or neutral, and a majority of individuals featured on podcasts do not disclose conflicts of interest, highlighting potential opportunities for improvement, including the need for standardization of financial conflict of interest disclosure.
Within oncology-related podcasts, the majority of conversations about oncologic drugs are perceived as either favorable or neutral, and a majority of individuals featured on podcasts do not disclose conflicts of interest, highlighting potential opportunities for improvement, including the need for standardization of financial conflict of interest disclosure.
