Edwardsrobertson7662

OBJECTIVE There are many predisposing factors associated with vaginismus, but there is lack of data in the literature regarding which and how of these factors influence the success rate of treatment. Our aim is to investigate the effects of factors that are considered as predisposing factors for vaginismus on treatment prognosis and success rate, with cognitive-behavioral therapy and desensitization exercises after sexual therapy. METHODS Patients with vaginismus were divided into three groups. Group 1 patients who successfully completed vaginal penetration exercises after sexual therapy and experienced vaginal sexual intercourse; Group 2 patients who started penetration exercises but could not reach success; Group 3 patients who discontinued treatment before starting exercises. Demographic and sexual parameters were compared between the groups. RESULTS There were statistically significant differences between the groups in terms of history of vaginismus in relatives (4.3%, 23% and 35.7%, p=0.047, respectively), the unsuccessful therapy history (69%, 61% and 21.4%, p=0.014, respectively), and anal and/or oral sex ratios (47.8%, 7.7% and 57.1%, p=0.019, respectively). Mean number of sessions were significantly higher in patients saying, "It is my fault" than among those perceiving it as a common problem (10.6±2.9 ve 7.5±5.7, p=0.042, respectively), and in patients with sexual disorder in their male partners than those not having any problem (13.3±3.7 ve 8.2±3.7, p=0.013, respectively). CONCLUSION Patients are more resistant to treatment if they have a history of vaginismus among relatives or when one of the couple say, it is his or her fault.OBJECTIVE To evaluate the impact of patient follicular environment with oxidative stress on oocyte quality. METHODS Patients on fertility treatment with either advanced maternal age or endometriosis were asked to donate follicular fluid collected during ovum pick-up. Follicular fluid (FF) was added (20%, 10% and 5%; %V/V) to in vitro maturation (IVM) medium with mouse oocytes. Following maturation culture, the oocytes were assessed for meiosis reinitiation. In a second setup, coenzyme Q10 was added to culture medium with FF. Nutlin-3a in vivo In addition to assessing meiotic maturation, a subset of oocytes was assessed for spindle structure and chromosome alignment. RESULTS Supplementation of IVM medium with FF of patients of advanced maternal age (with or without antioxidants) did not have an effect on the maturation capacity of mouse oocytes. However, the addition of FF of individuals with endometriosis (without antioxidants) in the two highest concentrations affected oocyte maturation (61.5% & 57.0% maturation) compared with the lowest concentration (89.2% maturation) (p less then 0.05). Supplementation of medium with coenzyme Q10 did not improve the maturation rate of oocytes exposed to the FF of individuals with endometriosis (28.5±13.7%) (p less then 0.05). Nevertheless, preliminary analysis of spindle abnormality and chromosome alignment revealed that oocytes resuming meiosis in the presence of FF of patients with endometriosis displayed aberrant spindle morphology and chromosomal misalignment. CONCLUSION The follicular environment of patients with endometriosis severely affected oocyte (nuclear) maturation. In vitro maturation in the presence of coenzyme Q10 appears to be a tool for rescuing oocytes exposed to such follicular environment.in English, Russian В последние годы широко изучается возможность применения интраназально вводимого инсулина для лечения болезни Альцгеймера и других когнитивных расстройств. В то же время практически не исследуется возможность его применения в рамках терапии сахарного диабета, что во многом связано с недостаточной изученностью молекулярных механизмов его действия на гормональный и метаболический статусы организма. В обзоре обсуждаются данные литературы и результаты собственных исследований о роли инсулина в центральной регуляции энергетического гомеостаза, а также об опыте применения интраназально вводимого инсулина для коррекции нарушений пищевого поведения и метаболических и гормональных дисфункций, развивающихся в условиях экспериментального сахарного диабета и метаболического синдрома. В исследованиях с участием здоровых добровольцев были показаны различные эффекты интраназально вводимого инсулина, в том числе влияние на когнитивные функции, пищевое поведение и снижение массы тела, а также обнаружена гендерная специфичность его действия. В ходе многочисленных исследований интраназально вводимого инсулина на животных моделях сахарного диабета были показаны не только стабилизация углеводного гомеостаза, но и положительное действие в виде восстановления функциональной активности инсулиновых сигнальных путей в гипоталамусе и других отделах мозга. Нами представлены и проанализированы данные о системных эффектах интраназально вводимого инсулина у грызунов с экспериментальными моделями сахарного диабета, а также у здоровых индивидуумов.