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We highlighted that SND occurred in low dissolved oxygen concentrations ( less then 1.5 mg L-1) for 120 days, without compromising aerobic granule integrity. These results suggest that the aerobic granular sludge process is a promising alternative for the co-treatment of landfill leachate and domestic wastewater under tropical climate conditions and its use should be encouraged.Mesenchymal stromal cells (MSCs) hold great promise in the field of regenerative medicine due to their ability to create a variable localized anti-inflammatory effect in injuries such as Crohn's disease and osteoarthritis or by incorporation in tissue engineered constructs. Currently, the MSC literature uses rodents for preclinical disease models. There is growing interest in using naturally occurring disease in large animals for modeling human disease. By review of the canine MSCs literature, it appears that canine MSCs can be difficult to maintain in culture for extended passages and this greatly varies between tissue sources, compared to human and rodent MSCs, and limited lifespan is an obstacle for preclinical investigation and therapeutic use [1]. Research using canine MSCs has been focused on cells derived from bone marrow or adipose tissue, and the differences in manufacturing MSCs between laboratories is problematic due to lack of standardization. To address these issues, here, a stepwise process was used to optimize canine MSCs isolation, expansion, and cryopreservation utilizing canine umbilical cord-derived MSCs. The culture protocol utilizes coating of tissue culture surfaces that increases cellular adherence, increases colony-forming units-fibroblast efficiency, and decreases population doubling times. Canine MSCs isolated with our protocol could be maintained longer than published canine MSCs methods before senescing. Our improved cryopreservation protocols produce on average > 90% viable MSCs at thaw. These methods enable master-bank and working-bank scenarios for allogeneic MSC testing in naturally occurring disease in dogs.Introduction Prior research has shown an association between breast and thyroid cancer although their relationship is unclear. In China, asymptomatic women undergoing regular health check-ups usually undergo breast and thyroid ultrasonography screening. The present cross-sectional ultrasound-based study estimated the prevalence of breast masses(BM) and thyroid nodules(TN) and their relationship among a population-based cohort of Chinese women. Methods This study included 34,184 consecutive asymptomatic Chinese women who underwent both breast and thyroid ultrasound evaluation during one health-care examination. Detected lesions were assigned into categories of different malignant risk according to the Breast and Thyroid Imaging Reporting and Data System(BI-/TI-RADS). Binomial logistic regression was used to determine the association between occurrence of BM and TN, and multinomial logistic regression was used to analyze the correlation of BM and TN in different BI-/TI-RADS categories. Associations between BM a higher RADS categories.AIM Evidence for the ideal/best practice priming solution remains meagre and largely historical. The aim of this survey was to determine the constituents of contemporary priming solutions in adult open-heart centres across Australia. This would provide insight on the level of variation within current Australian priming practices and inform perfusionists of how their current priming methods compare to the spectrum of Australian practice. METHOD A total of 15 survey questions covering various aspects of priming constituents were sent via email to perfusionists in all 63 adult open-heart centres across Australia. RESULTS This prime survey received a 100% response rate across Australia. All units prime with a balanced physiological solution, 73% of units prime with Plasma-Lyte 148 and 19% with Hartmann's solution. No synthetic colloids are used for priming in Australia. Up to 6,520 (30%) cases per annum receive heparin as the only additive to their prime base solution. All other cases had various combinations of sodium bicarbonate, mannitol and albumin added for a variety of recorded reasons. CONCLUSION Contemporary Australian priming practices show a marked level of conformity between units. Variation exists in the rationale for adding sodium bicarbonate, mannitol and albumin. Further investigations into the clinical effects of these additives are required to determine if the rationale for their addition is historical or judicious in this contemporary era of low prime volumes, physiological base solutions and coated bypass circuits.Aim The aim of this study is to verify the type of scaffold effect on tissue engineering for dentine regeneration in animal models. Materials & methods Strategic searches were conducted through MEDLINE/PubMed, Web of Science and Scopus databases. The studies were included with the following eligibility criteria studies evaluating dentine regeneration, and being an in vivo study. Results From 1392 identified potentially relevant studies, 15 fulfilled the eligibility criteria. All studies described characteristics of neoformed dentine, being that the most reported reparative dentine formation. Most of included studies presented moderate risk of bias. Conclusion Up to date scientific evidence shows a positive trend to dentine regeneration when considering tissue engineering in animal models, regardless the type of scaffolds used.Background Violence against women maintains its importance. Nursing and midwifery students should be able to identify symptoms of violence against women. Healthcare providers have received insufficient education, either before or after graduation, for identifying violence cases and for dealing with interfamilial violence against women.Aims This study is conducted to identify nursing and midwifery students' ability to recognise symptoms of violence against women.Methods This cross-sectional study included nursing and midwifery students attending the Ege University, School of Nursing, the Celal Bayar University, Health School of Higher Education and Health School of Izmir Atatürk (N = 557). The convenience sampling method was used. There were 114 students who declined to participate in this study as they did not want to answer questions about violence (n = 443).Results It was determined that 94.6% of the students were knowledgeable about violence against women. selleckchem The scores obtained by students from the physical symptom sub-dimension were 8.