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s the first report of C. siamense causing fruit anthracnose on guava in Mexico. Therefore, it is necessary to explore the diversity of Colletotrichum species on guava in detail through subsequent phylogenetic studies as well as to monitor the distribution of this pathogen into other Mexican regions.Mizuna (Brassica rapa var. japonica), a member of family Brassicaceae, is a leafy vegetable having phenolic and other compounds beneficial to human health, such as natural antioxidants (Khanam et al. 2012). In October 2020, a field of mizuna (variety Early) on Oahu island was observed having 20-30% diseased plants. Four randomly selected infected mizuna plants, showing the symptoms of wilt and stem rot (Figure 1A-D), were collected and isolations were made to determine the pathogen. Small sections of infected stems were cut, surface sterilized with 0.6% sodium hypochlorite solution for 30 sec, followed by three consecutive rinses in distilled water. The tissues were macerated in a sterile 1.5 ml centrifuge tube containing 100 μl sterile water-macerated tissues were streaked onto crystal violet pectate medium (CVP) (Hélias et al. 2011) and incubated at 26 ± 2°C for 48 h. Isolated bacterial colonies that formed pits on the CVP plates were re-streaked onto dextrose peptone agar Peptone (10 g/L), Dextrose (5 g/L)nd gapA), the bacterial strains from mizuna grouped with other P. brasiliense retrieved from the NCBI GenBank database. To our knowledge, this is the first report of P. brasiliense infecting mizuna plants in Hawaii or in the USA and is important because this species is one of the most aggressive pectolytic pathogens in the genus Pectobacterium. Understanding the diversity of different pectolytic phytopathogens is essential to formulating risk mitigation strategies as P. brasiliense could potentially pose a threat to additional vegetable crops, especially the crucifers vegetables (Arizala et al. 2019; Klair et al, 2021).Maize (Zea mays) is the second most cultivated grain crop in Ecuador, with growing significance as a source of fodder and food. During the rainy season (November and December) of 2018 and 2019, a disease of maize that was not previously observed in Ecuador was found at commercial fields of Misqui Sara variety, at four parishes of canton Quito (Tumbaco, Pifo, Puembo, and Checa), province of Pichincha. Infected plants, at tassel initiation, displayed symptoms of localized chlorotic streaks on leaves that expanded with time, and around a month later turned necrotic. Severely affected plants wilted and died. Symptoms appeared in lower leaves first and were later observed in upper leaves as the disease progressed. Disease incidence was between 20 and 30% in the affected plantations, with around 30% of infected plants wilting and dying, resulting in 20-25% of yield losses. Upper leaves from ten symptomatic plants, five from Puembo and five from Checa, were collected randomly. Two 0.5 cm2 pieces of leaf from each pl knowledge, this is the first report of P. ananatis causing leaf spot of maize in Ecuador.Gladiolus (Gladiolus gandavensis Van Houtte) is a perennial plant in the family Iridaceae, which shows sword-shaped leaves and spikes of brilliantly colored irregular flowers arising from corms. It is one of the most important fresh cut flowers and is widely cultivated worldwide, including in China. In September 2020, white pinpoints were first observed on gladiolus leaves in Jiangmen City, Guangdong Province, China. The white spots eventually turned brown. The lesions then developed into oval to circular spots, which were surrounded with an obvious yellow halo. The spots expanded and coalesced, causing leaf blight. These symptoms were observed on approximately 10% of gladiolus plants in fields measuring ca. 70 ha. Symptomatic leaves were sampled from fields, surface sterilized in 75% ethanol for 30 s, submerged in a 2% NaOCl solution for 10 min, and rinsed three times with sterile water. The samples were then cut into pieces (5 × 5 mm) and incubated for 4 d on potato dextrose agar (PDA) at 25°C. A representarecorded as a Curvularia species occurring in China (Zhang et al. 2006), it was not reported to cause leaf spots on gladiolus in Guangdong Province and elsewhere in China. To our knowledge, this is the first report of Curvularia gladioli causing leaf spots on gladiolus in China. Identification of this pathogen will help develop diagnostic methods for corms and seedlings, and may lead to the development of appropriate chemical management strategies.In September 2020, a disease resembling stem blight on soybean was found in Chengdu city, Sichuan province, southwestern of China. Symptoms began as a brown lesion on the stems, usually at the nodes, then lesions expanded, darkened, and even girdled the stems, causing wilt of the above stems (Figure 1A). In three 0.33-ha fields, a total of 300 soybean plants (20 plants/site × 5 sites/filed × 3 fields) were investigated, and 3% of the plants showed the disease symptoms. The symptoms were consistent with those previously reported for stem canker and stem blight on soybean caused by Diaporthe complex (Cui et al. 2009; Mena et al. 2019; Santos et al. 2011). The tissues of symptomatic soybean stems were rinsed by water, disinfected by submerging them in 75% ethanol for 30 s and in 2% sodium hypochlorite solution for 2 min, then followed by washing with sterile distilled water. Small diseased tissue fragments were placed on selective potato dextrose agar (PDA) containing rifampicin and ampicillin (both 50 mg/μl). Peful information for soybean disease control in this region of China. The authors declare no conflict of interest. Funding China Agriculture Research System (CARS-004-PS14).Background Crural repair is an essential technical component in laparoscopic hiatal hernia surgery, but there is no consensus regarding the optimal method to prevent postoperative hernia recurrence. check details Mesh augmentation, especially with permanent materials, is associated with dysphagia and complications. The rotational falciform ligament flap (FLF) has been reported to be effective in reinforcing standard suture closure of the hiatus. Materials and Methods Patients with primary or secondary hiatal hernia in whom FLF was used to buttress the hiatus repair were included. The FLF was dissected from the anterior abdominal wall, detached from the umbilical area, and transposed below the left lateral liver segment to buttress the cruroplasty. Indocyanine green fluorescence was used to assess vascularization of the flap before and after mobilization. Results Eighteen consecutive patients underwent laparoscopic FLF cruroplasty reinforcement between October 2019 and January 2021. Indications were primary hiatal hernia (n = 9), recurrent hiatal hernia (n = 4), postsleeve gastrectomy hernia (n = 1), prophylactic hiatal repair during esophagectomy and gastric conduit reconstruction (n = 2), and postesophagectomy hernia (n = 2). All flaps were well vascularized and covered the entire hiatal area. There was no morbidity. At a median follow-up of 8 months (range 3-15), the symptomatic and quality of life scores significantly improved compared with baseline (P  less then  .001), and no anatomic hernia recurrences were detected. Conclusions FLF is safe for crural buttress and is a viable alternative to mesh in laparoscopic hiatal hernia surgery.Fusarium redolens was previously reported as a plant pathogen or an endophyte that is closely related to F. oxysporum, a notoriously significant soil-borne phytopathogen. Subsequent studies demonstrated the unique nature of F. redolens and was considered a distinct species that causes multiple symptoms on multiple hosts. It was recently identified as a pathogen that causes root rot of American ginseng. Currently, few high-quality F. redolens genome sequences exist in the public database. Here, we report the whole genome sequence of F. redolens strain YP04, based on a hybrid assembly long- and short-read sequencing with PacBio and Illumina platforms, respectively. The assembly consists of 40 configs with a total length of 52.8 Mb nuclear genomic DNA and 49.6 kb complete mitochondrial genomic DNA, and encodes a total of 18,985 genes including 18,517 protein-coding genes and 469 RNA genes which were functionally annotated. A total of 4606 proteins were identified in the pathogen host interactions (PHI) database, suggesting they were likely involved in pathogenicity and host-pathogen-host interactions, while 41 secondary metabolite synthesis clusters were predicted and annotated. This is the first high-quality whole genome of F. redolens, providing an important community resource for genome evolution, host-pathogen-host interaction, and secondary metabolite biosynthesis studies.Background To investigate the application value of 4K high definition (HD) in laparoscopic gastrectomy by comparing the short-term outcomes and subjective perception with three-dimensional (3D) and HD vision systems. Materials and Methods A retrospective study was conducted between September 2018 and February 2019; a total of 87 patients who underwent laparoscopic gastrectomy were enrolled and divided into three groups in terms of different type of vision system used for surgery 4K, 3D, and HD. Demographic and clinicopathological data as well as short-term outcomes were collected and analyzed. A questionnaire survey was completed by the team of surgeons to evaluate the subjective perception of different vision systems. Results There was no significant difference in gender, body mass index, age, American Society of Anesthesiologists (ASA) score and history of abdominal surgery, tumor location as well as type of operation, and anastomosis between the 4K, 3D, and HD groups. All patients underwent laparoscopic gaded the highest in terms of control co-ordination of visual angle, visual acuity, radiance, resolution ratio and frames, and refresh rate. HD group was graded significantly lower in sense of control compared with that of 4K and 3D group. No significant difference was found in color resolution and contrast. Conclusions In conclusion, the short-term effect of 4K HD laparoscopic system is comparable with that of HD and 3D laparoscopy, whereas 4K could reduce adverse effect than traditional instrument and improve quality of surgery. The Clinical Trial Registration number is NCT01441336.Background Vibrio vulnificus is a rare but life-threatening infection that effects the population near warm coastal areas. This infection could be fulminant and rapidly progress to severe sepsis and necrotizing soft tissue infection. Early diagnosis and treatment are critical to saving patients' lives. With multiple studies reporting discrepancies in prognostic factors and different treatment protocols, we aimed through this meta-analysis to assess these factors and protocols and the impact on the outcome of the infection. Materials and Methods In accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic search of PubMed, Embase, and Cochrane Library databases by two independent reviewers was reported. Studies evaluating prognostic factors and treatment outcomes of Vibrio vulnificus infections were included. Comprehensive Meta-Analysis, version 3.0 was used. Results Two hundred eleven studies were identified. Of those, eight studies met our inclusion criteria.