Denckerphillips8675

These results reveal the presence of a G4 motif in the TYMS gene, probably involved in the autoregulation of TYMS expression, and the therapeutic potential of a PPRH targeted to the G4FS.Etlingera pavieana (Pierre ex Gagnep.) R.M.S. is a rhizomatous plant in the Zingiberaceae family which could be freshly eaten, used as a condiment or as a traditional remedy. Our work investigated the chemical composition and antimicrobial activity of the E. pavieana essential oils extracted from the rhizome (EOEP). We extracted the EOEP from the rhizome by hydrodistillation and analyzed the chemical composition by headspace solid-phase microextraction coupled with gas chromatography/mass spectrometry (HS-SPME-GC/MS). A total of 22 volatile compounds were identified where trans-anethole (78.54%) and estragole (19.36%) were the major components in the EOEP. The antimicrobial activity of EOEP was evaluated based on the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values using the broth dilution method and enumerating cell death overtime. Our work shows that the EOEP exhibits potent antibacterial activity against foodborne pathogenic Gram-positive bacteria, namely Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes in the range of 0.1-0.3% (v/v). We further investigated the mechanism of EOEP inhibition using Synchrotron Fourier transformation infrared (FTIR) microspectroscopy. Here, we show significant differences in DNA/nucleic acid, proteins and cell membrane composition in the bacterial cell. To conclude, EOEP exhibited antimicrobial activity against foodborne pathogens, especially the Gram-positive bacteria associated with ready-to-eat (RTE) food and, thus, has the potential to serve as a natural preservative agent in RTE products.Infection with Lumpy Skin Disease virus (LSDV), as well as infections with other Capripox virus species, are described as the most severe pox diseases of production animals and are therefore listed as notifiable diseases under the guidelines of the World Organization for Animal Health (OIE). To our knowledge there is only a single study examining dose dependency, clinical course, viremia, virus shedding, as well as serological response following experimental LSDV "Neethling" inoculation. Here, we inoculated cattle with four different doses of LSDV strain "Macedonia2016", a recently characterized virulent LSDV field strain, and examined clinical symptoms, viremia, viral shedding, and seroconversion. Interestingly, around 400 cell culture infectious dose50 (CCID50) of LSDV-"Macedonia2016" were sufficient to induce generalized Lumpy Skin Disease (LSD) in two out of six cattle but with a different incubation time, whereas the other animals of this group showed only a mild course of LSD. However, differences in incubation time, viral loads, serology, and in the clinical scoring could not be observed in the other three groups. In summary, we concluded that experimental LSDV infection of cattle with an infectious virus titer of 105 to 106 CCID50/mL of "Macedonia2016" provides a robust and sufficient challenge model for future studies.We studied the use of a rotating multi-layer 3D Light Detection And Ranging (LiDAR) sensor (specifically the Velodyne HDL-32E) mounted on a social robot for the estimation of features of people around the robot. While LiDARs are often used for robot self-localization and people tracking, we were interested in the possibility of using them to estimate the people's features (states or attributes), which are important in human-robot interaction. In particular, we tested the estimation of the person's body orientation and their gender. As collecting data in the real world and labeling them is laborious and time consuming, we also looked into other ways for obtaining data for training the estimators using simulations, or using LiDAR data collected in the lab. We trained convolutional neural network-based estimators and tested their performance on actual LiDAR measurements of people in a public space. The results show that with a rotating 3D LiDAR a usable estimate of the body angle can indeed be achieved (mean absolute error 33.5 ° ), and that using simulated data for training the estimators is effective. For estimating gender, the results are satisfactory (accuracy above 80%) when the person is close enough; however, simulated data do not work well and training needs to be done on actual people measurements.Aiming at the poor accuracy and difficult verification of maneuver modeling induced by the wind, waves and sea surface currents in the actual sea, a novel sea trials correction method for ship maneuvering is proposed. The wind and wave drift forces are calculated according to the measurement data. Based on the steady turning hypothesis and pattern search algorithm, the adjustment parameters of wind, wave and sea surface currents were solved, the drift distances and drift velocities of wind, waves and sea surface currents were calculated and the track and velocity data of the experiment were corrected. The hydrodynamic coefficients were identified by the test data and the ship maneuvering motion model was established. The results show that the corrected data were more accurate than log data, the hydrodynamic coefficients can be completely identified, the prediction accuracy of the advance and tactical diameters were 93% and 97% and the prediction of the maneuvering model was accurate. Numerical cases verify the correction method and full-scale maneuvering model. The turning circle advance and tactical diameter satisfy the standards of the ship maneuverability of International Maritime Organization (IMO).The metabolic requirements of metastatic non-small cell lung (mNSCLC) tumors from patients receiving first-line platinum-doublet chemotherapy are hypothesized to imprint a blood signature suitable for survival prediction. Pre-treatment samples prospectively collected at baseline from a randomized phase III trial were assayed using nuclear magnetic resonance (NMR) spectroscopy (n = 341) and ultra-high performance liquid chromatography - mass spectrometry (UPLC-MS) (n = 297). Distributions of time to event outcomes were estimated by Kaplan-Meier analysis, and baseline characteristics adjusted Cox regression modeling was used to correlate markers' levels to time to event outcomes. Sixteen polar metabolites were significantly correlated with overall survival (OS) by univariate analysis (p less then 0.025). Formate, 2-hydroxybutyrate, glycine and myo-inositol were selected for a multivariate model. TIC10 mouse The median OS was 6.6 months in the high-risk group compared to 11.4 months in the low risk group HR (Hazard Ratio) = 1.