Curryenemark0649

720-0.946), instead of other populations. CONCLUSIONS In summary, findings from this study suggested that higher dietary intake of vitamin A may contribute to the lower development of ovarian cancer, especially among North Americans. Copyright 2020 The Author(s).BACKGROUND Approximately 40% of all Enterobacterales (EB) bloodstream infections (BSI) among solid organ transplant recipients (SOTR) are due to extended-spectrum beta-lactamase (ESBL)-producing organisms, but risk factors for such infections remain ill-defined in this population. Thus, we sought to determine the risk factors for ESBL-EB BSI among SOTR. METHODS A multicenter case-control study was performed. All SOTR with an EB BSI at the Hospital of the University of Pennsylvania and University of Maryland Medical Center between January 1, 2007 and June 30, 2018, and at The Johns Hopkins Hospital between January 1, 2005 and December 31, 2015, were included. Cases were those with an ESBL-EB BSI. Controls were those with a non-ESBL-EB BSI. Multivariable logistic regression was performed to determine risk factors for ESBL-EB BSI. RESULTS There were 988 episodes of EB BSI, of which 395 (40%) were due to an ESBL-EB. On multivariable analysis, the independent risk factors for ESBL-EB BSI included ESBL-EB on prior culture (aOR 12.75, 95% CI 3.23-50.33, P less then 0.001); a corticosteroid-containing immunosuppression regimen (aOR 1.30, 95% CI 1.03-1.65, P=0.030); acute rejection treated with corticosteroids (aOR 1.18, 95% CI 1.16-1.19, P less then 0.001); and exposure to third-generation cephalosporins (aOR 1.95, 95% CI 1.48-2.57, P less then 0.001), echinocandins (aOR 1.61, 95% CI 1.08-2.40, P=0.020), and trimethoprim-sulfamethoxazole (aOR 1.35, 95% CI 1.10-1.64, P=0.003). CONCLUSIONS We identified several novel risk factors that are uniquely important to the SOTR population, including exposure to trimethoprim-sulfamethoxazole and corticosteroid-containing immunosuppressive regimens. Further studies exploring these associations and testing interventions aimed at these modifiable risk factors among SOTR are needed. © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.Colorectal cancer (CRC) is the third most common malignancy and one of the leading causes of cancer-related death among men worldwide. CRC is a multifactor digestive pathology, which is a huge problem faced not only by clinicians but also by researchers. Importantly, a unique feature of CRC is the dysregulation of molecular signaling pathways. To date, a series of reviews have indicated that different signaling pathways are disordered and have potential as therapeutic targets in CRC. Nevertheless, an overview of the function and interaction of multiple signaling pathways in CRC is needed. Therefore, we summarized the pathways, biological functions and important interactions involved in CRC. First, we investigated the involvement of signaling pathways, including Wnt, PI3K/Akt, Hedgehog, ErbB, RHOA, Notch, BMP, Hippo, AMPK, NF-κB, MAPK and JNK. Subsequently, we discussed the biological function of these pathways in pathophysiological aspects of CRC, such as proliferation, apoptosis and metastasis. Finally, we summarized important interactions among these pathways in CRC. We believe that the interaction of these pathways could provide new strategies for the treatment of CRC. © 2020 The Author(s).Glycans are known to be involved in many biological processes, while little is known about the expression of N-glycans during vertebrate development. We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog. N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry. We quantified 110 N-glycan compositions that spanned four orders of magnitude in abundance. Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated. The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming. An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41). Two notable and opposing regulation events were detected for sialylated glycans. LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species. The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots. We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage). Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.Arsenic exists ubiquitously in the soil and has been proved to be of significant hazard to human health upon transmission through food chain. Herein, we determined the effects of Fe-Mn-La ternary oxide-biochar composites (FMLBCs) on arsenic (As) fractionation, soil enzyme activities, and microbial communities in arsenic-polluted soils. The results demonstrated that the proportion of non-swappable As fractions reduced and that of the exchangeable As fractions increased with the addition of FMLBCs. Furthermore, the addition of FMLBCs significantly increased the catalase (CAT) activity (P less then 0.05), and an increase of 69.2-268% was observed when 2 wt% FMLBCs were added. Supplementation with biochar or FMLBCs increased the relative abundance of Proteobacteria and Acidobacteria and decreased the relative abundance of Firmicutes; moreover, the effect was more obvious as the addition amount of biochar or FMLBCs increased. In addition, the FMLBCs, except for FMLBC3, increased the content of available phosphorus. Moreover, amendments of FMLBCs led to an increase in the available potassium content by an average of 212%, 113%, and 62.1% in highly polluted soil. Therefore, the FMLBCs affected the physical and chemical properties of soil in different manners. The results suggested that the addition of FMLBCs changed the distribution and increased the immobilization of As in the soil; this could indirectly reduce the risk of the transport of As to rice. The amendment mechanism of FMLBCs may include changes to the physicochemical soil properties and consequently, the soil enzyme activities are affected, which can influence the microbial communities in soils.The subtle structural change of hydrophilic ligands on the size control of metal nanoclusters (NCs) is unclear but critically important for fundamental understanding. Herein, we report our findings that subtle changes of isomeric ligands lead to a dramatic difference in the size of water-soluble Au NCs. By using isomeric para-mercaptobenzoic acid (p-MBA), m-MBA, and o-MBA as model ligands, it was found that both the steric hindrance and the electronic effect of isomeric ligands significantly influences the size of Au NCs, resulting in the formation of different sized Au44(p-MBA)26 NCs, Au25(m-MBA)18 NCs, and Au37/43(o-MBA)22/26 NCs. Besides this, by collocating any two of the isomeric MBAs as ligand pairs to compare their protecting capability for Au NCs, the protecting abilities of such ligands were found to follow the trend m-MBA > o-MBA > p-MBA. In addition, the growth process of Au44(p-/o-MBA)26 NCs from Au(i)-MBA complexes in the NaBH4 reduction system was also monitored by real-time UV-vis absorption spectroscopy and ESI mass spectrometry, which complies with the 2e- hopping growth principle, indicating the universal applicability of this principle in the synthesis of thiolated metal NCs. learn more This study provides a fundamental understanding of the effect of ligands' steric hindrance and electronic factors on the size control of water-soluble metal NCs and sheds light on the formation of metal NCs in the NaBH4 reduction system.In mammals, it is believed that the intercellular coupling mechanism between neurons in the suprachiasmatic nucleus (SCN) confers robustness and distinguishes the central clock from peripheral circadian oscillators. Current in vitro culturing methods used in Petri dishes to study intercellular coupling by exogenous factors invariably cause perturbations, such as simple media changes. Here, we design a microfluidic device to quantitatively study the intercellular coupling mechanism of circadian clock at the single cell level, and demonstrate that vasoactive intestinal peptide (VIP) induced coupling in clock mutant Cry1-/- mouse adult fibroblasts engineered to express the VIP receptor, VPAC2, is sufficient to synchronize and maintain robust circadian oscillations. Our study provides a proof-of-concept platform to reconstitute the intercellular coupling system of the central clock using uncoupled, single fibroblast cells in vitro, to mimic SCN slice cultures ex vivo and mouse behavior in vivo phenotypically. Such a versatile microfluidic platform may greatly facilitate the studies of intercellular regulation networks, and provide new insights into the coupling mechanisms of the circadian clock.Asymmetric obstacles can be exploited to direct the motion and induce sorting of run-and-tumble particles. In this work, we show that flocking particles which follow the Vicsek model aligning rules experience collective trapping in the presence of a wall of funnels made of chevrons, concentrating at the opposite side of the wall of funnels to run-and-tumble particles. Flocking particles can be completely trapped or exhibit a dynamical trapping behaviour; these two regimes open the door to the design of a system with two perpendicular flows of active particles. This systematic study broadens our understanding of the emergence of collective motion of microorganisms in confined environments and directs the design of new microfluidic devices able to control these collective behaviours.Susceptibility of traditional hydrogels to water leads to the deterioration of their mechanical properties and dimensional instability. Inspired by bone tissues, here, we report a nonswellable gradient hydrogel with adjustable mechanical properties via a simple acid-heat treatment of polyamide-based hydrogels. Both the hydrophilicity/hydrophobicity and crosslinking degree of the gel can be simultaneously regulated by taking advantage of the conversion of amide groups to imide groups in the hydrogel. A gradient imide structure is formed with a dense layer near the surface of the gel. The resultant hydrogel has no size change both in water and 0.9 wt% NaCl solution while it shows high strength with a compressive stress of 70 MPa at 70% strain at a higher imidization degree. At a lower imidization degree, the gel has excellent fatigue resistance and resilience and can return to its original state after 5 cycles of 90% strain.