Cruzmarshall3799

CANTRIP, which combines two detection platforms consisting of CRISPR-Cas12a and fluorescent CuNPs into a single reaction, appears to be a robust, low-cost and simple diagnostic tool.In rotator cuff repair, strong and reliable suturing is necessary to decrease failure rates. The biomechanics of two self-cinching stitches - the single-loop knot stitch (SLKS) and the double-loop knot stitch (DLKS) - and the modified Mason-Allen stitch (mMAS) were compared. Twenty-seven porcine infraspinatus tendons were randomized among the three stitches. Each was cyclically loaded (10-80-200 N for 50 cycles each) while the gap formation was measured. Next, ultimate load to failure was tested. The gap widths after cyclic loading were 8.72 ± 0.93 mm for the DLKS, 8.65 ± 1.33 mm for the mMAS, and 9.14 ± 0.89 mm for the SLKS, without significant differences. The DLKS showed the highest ultimate load (350.52 ± 38.54 N) compared with the mMAS (320.88 ± 53.29 N; p = 0.304) and the SLKS (290.54 ± 60.51 N; p less then 0.05). The DLKS showed similar reliability and better strength compared with the mMAS, while the SLKS showed a slight but not significant decrease in performance. In our experience, the DLKS and SLKS have clinical advantages, as they are easy to perform and the self-cinching loop knot allows the surgeon to grasp degenerative tendon tissue. Initial intraoperative tightening of the suture complex (preloading) before locking is important in order to decrease postoperative elongation.Alpine musk deer, Moschus chrysogaster, a solitary, primitive ungulate inhabiting high elevation areas (3000-4500 m) is an endangered species facing threat of extinction globally due to excessive hunting for its musk. In this study, we determined the complete mitochondrial genome of M. chrysogaster, which was 16,354 bp in length, and revealed the same gene order and genomic organization as typical Moschidae mitochondrial DNA. Start codons in 13 protein-coding genes (PCGs) were all typical ATGs except ATA for ND2 and ND3 and ATT for ND5. Stop codons were all typical types except an incomplete stop codon T for COX3, ND2, ND3, and ND4. Secondary structures in 22 transfer RNA genes all showed typical cloverleaf except tRNA-Ser (AGY), in which the dihydrouridine arm formed a simple loop. No repeat units were found in the control region. The topology structure indicated that M. cupreus was primitive and located at the root of the Moschidae clade. Phylogenetic reconstruction placed M. chrysogaster as a distinct lineage, closely related to the branch of M. leucogaster, M. berezovskii (wild) and predicted a sister relationship with M. moschiferus, M. anhuiensis, and M. berezovskii (captive). (R,S)-3,5-DHPG chemical structure However, we suggested that the genetic resources of M. chrysogaster_JQ608470 should be further investigated.In this study, the complete mitochondrial genome of Arhopalus unicolor was first sequenced. The length of the complete mitochondria genome of A. unicolor was 15,760 bp with 19.0% GC content, including 40.5% A, 10.8% C, 8.2% G and 40.5% T. There were 13 protein-coding genes (CDS), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and an AT-rich region. Phylogenetic analysis showed that A. unicolor was closely related to Spondylis buprestoides. This study provides useful genetic information for subsequent prevention of A. unicolor.The genus Crematogaster is a diverse group of ants found around the world. We have completed the mitochondrial genome of Crematogaster teranishii, which is the first mitochondrial genome of the genus. The mitochondrial genome is 17,442 bp long and 20.3% in GC ratio, which is similar to those of other ants. It contains 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a control region with same gene order to other myrmicine species. The intergenic region between nad3 and trnA was unusually long compared to other ant species. Phylogenetic analysis showed that C. teranishii was closely related to other members of tribe Crematogastrini.Laevistrombus canarium is a marine gastropod species with high economical value. The complete mitochondrial genome of L. canarium has been characterized in this study. The circular mitogenome is 15626 bp in length and comprises 13 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA (rRNA) genes. The organization of these genes is consistent with that of other stromboidae species. The overall base composition of mitochondrial genome is 30.87% A, 38.99% T, 15.54% G, and 14.60% C, with 69.86% AT. Phylogenetic analysis further implies that L. canarium is placed within the Stromboidae.The Gymnobelideus leadbeateri (Leadbeater's Possum) is listed as Critical Endangered on the International Union for Conservation of Nature (IUCN) Red List. We assembled the complete mitochondrial genome for the G. leadbeateri and characterized it to provide informative data for forthcoming studies for understanding its evolution and conservation genetics. The G. leadbeateri mitogenome is 16,812 bp long and encodes 13 protein-coding genes (PCGs), two ribosomal RNA genes (12S rRNA and 16S rRNA) and 22 transfer RNA (tRNA) genes. Phylogenetic analysis based on complete mitogenome shows that G. leadbeateri is related to Petaurus breviceps (sugar glider) and Dactylopsila trivirgata (striped possum).The mitochondrial genome of Beauveria lii, strain RCEF500, was sequenced on the NovaSeq 6000 and the Nanopore Sequencer, and annotated. The genome is 59,014 bp in length, encoding 15 conserved protein-coding genes (PCGs), 2 rRNA genes and 23 tRNA genes. The nucleotide composition of Beauveria lii mitochondrial genome was 38.23% of A, 35.81% of T, 11.61% of C, 14.36% of G, 25.97% of G + C content. Phylogenetic analysis confirmed B. lii as a member of Beauveria (Cordycipitaceae). link2 The mitochondrial genome of B. lii will contribute to the understanding of phylogeny and evolution of the genus and family.Adonis amurensis Regel et Radde is an important cardiac folk medicinal plant which endemic to Northeast Asia. We determined the first complete chloroplast genome of A. amurensis using genome skimming approach. The cp genome was 157,032 bp long, with a large single-copy region (LSC) of 86,218 bp and a small single-copy region (SSC) of 18,212 bp separated by a pair of inverted repeats (IRs) of 26,301 bp. It encodes 129 genes, including 84 protein-coding genes, 37 tRNA genes, and 8 ribosomal RNA genes. We also reconstructed the phylogeny of Adonideae and Isopyreae using maximum likelihood (ML) method, including our data and previously reported cp genomes of related taxa. The phylogenetic analysis indicated that A. amurensis is close related with Adonis sutchuenensis.The complete mitochondrial genome of the Baillon's Crake Porzana pusilla (Gruiformes Rallidae) are sequenced and annotated, which contained 37 typical genes. The length of the complete mitochondrial genome is 16,966 bp (GenBank No. MW043485), with As, Ts, Cs, Gs, and AT content of the mitochondrial genome is 32.1, 23.2, 30.9, 13.8, and 55.3%, respectively. All protein-coding genes started with ATN except COX1 and ND5, which start with GTG, and all protein-coding genes end with a complete triplet codon (TAA, AGG, AGA, and TAG), except COX3, which ends with an incomplete T. The ND3 gene of P. pusilla with an extra C nucleotide in 174 site. Phylogenetic analysis revealed that the new sequenced species of P. pusilla was closer to the clade of Porzana fusca and Porzana paykullii, and all three Porzana are clustered into one branch.Bar-tailed Treecreeper Certhia himalayana usually lives in coniferous or mixed broadleaf-conifer forests, often crawling along the trunk. In this study, we first sequenced and described the complete mitochondrial genome and phylogeny of C. himalayana. The whole genome of C. himalayana was 16,852 bp in length, and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosome RNA genes, and 1 non-coding control regions. The overall base composition of the mitochondrial DNA was 25.1% for A, 29.2% for T, 14.5% for C, 31.2% for G, with a GC content of 45.7%. A phylogenetic tree strongly supported that C. himalayana closely related with Family Troglodytidae by highly probability.Cacopsylla citrisuga (Yang & Li) is an important pest-threatening Citrus and Poncirus plants (Rutaceae) and a newly identified insect vector of citrus Huanglongbing. The complete mitochondrial genome of C. citrisuga was 14,906 bp in length, with 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs). The phylogenetic trees inferred from Bayesian inference and maximum likelihood analyses confirmed C. citrisuga as a member of the genus Cacopsylla. Our phylogenetic analyses suggested that the Cacopsylla is paraphyletic, and confirmed C. citrisuga as a member of clade-I under Cacopsylla. The complete mitochondrial genome of C. citrisuga will provide important information for the phylogeny and evolution analysis of Cacopsylla.Completed chloroplast genome of Zoysia matrella (L.) Merr. isolated in Korea is 135,888 bp long (GC ratio is 38.4%) and has four subregions 81,370 bp of large single copy (36.3%) and 12,594 bp of small single copy (32.7%) regions are separated by 20,962 bp of inverted repeat (44.1%) regions including 130 genes (83 protein-coding genes, eight rRNAs, 38 tRNAs, and one pseudogene). 28 SNPs and 57 INDELs were identified ss intraspecific variations against previously sequenced chloroplast genome. Phylogenetic trees show that Z. link3 matrella and Z. tenuifolia (=Z. pacifica) are clustered in one clade with low level of variations on chloroplast genomes.The complete mitochondrial DNA (mtDNA) for Odorrana exiliversabilisLi, Ye and Fei 2001 (Anura Ranidae) was determined in this study. The length of complete mtDNA was 17,122 bp, including 13 PCGs (COI-III, ND1-6, ND4L, ATP6, ATP8 and CYTB), 25 tRNA genes, 2 rRNA genes, 2 non-coding regions of a L-strand replication origin and a control region. The overall base composition of the sequence is 28.27% T, 28.27% C, 28.52% A, and 14.94% G, with a total A + T content of 56.79%. The phylogenetic tree showed that O. exiliversabilis was the sister species of O. tormota, and formed a monophyletic clade with other Odorrana species. These data provide a powerful tool for evolutionary biology and population genetics of genus Odorrana.Penthimia is the largest genus in the tribe Penthimiini of the subfamily Deltocephalinae. To date, there are no available mitogenome sequences from Penthimia. In this study, we have sequenced and annotated the complete mitogenome of Penthimia melanocephala (Motschulsky 1863), the mitogenome is 15,308 bp in length, which including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNAs (tRNAs) and a long non-coding region (control Region), base composition of whole sequences are A (50.4%), C (15.3%), G (10.0%), and T (24.3%). All PCGs started with the typical ATN codon and stopped with the typical TAN codon except for COII and COIII stopped with single T. Within phylogenetic tree, Deltocephalinae members were clustered into a clade. The complete mitogenome of P. melanocephala can provide essential DNA molecular data for further evolutionary and phylogenetic analysis.