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Under chilling stress, antioxidant enzyme activities (peroxidase (POD) and catalase (CAT)) were downregulated in LJ11 compared with LJ25. However, the content of malondialdehyde (MDA) was higher in LJ11 compared with LJ25. Collectively, our findings identify low temperature responsive genes that can be effectively used as candidate genes for molecular breeding programmes to increase the chilling tolerance of rice.Telomeres are repeat sequences of non-coding DNA-protein molecules that cap or intersperse metazoan chromosomes. Interest in telomeres has increased exponentially in recent years, to now include their ongoing dynamics and evolution within natural populations where individuals vary in telomere attributes. Phylogenetic analyses show profound differences in telomere length across non-model taxa. However, telomeres may also differ in length within individuals and between tissues. The latter becomes a potential source of error when researchers use different tissues for extracting DNA for telomere analysis and scientific inference. A commonly used tissue type for assessing telomere length is blood, a tissue that itself varies in terms of nuclear content among taxa, in particular to what degree their thrombocytes and red blood cells (RBCs) contain nuclei or not. Specifically, when RBCs lack nuclei, leucocytes become the main source of telomeric DNA. RBCs and leucocytes differ in lifespan and how long they have been exposed to 'senescence' and erosion effects. We report on a study in which cells in whole blood from individual Australian painted dragon lizards (Ctenophorus pictus) were identified using flow cytometry and their telomere length simultaneously measured. Lymphocyte telomeres were on average 270% longer than RBC telomeres, and in azurophils (a reptilian monocyte), telomeres were more than 388% longer than those in RBCs. If this variation in telomere length among different blood cell types is a widespread phenomenon, and DNA for comparative telomere analyses are sourced from whole blood, evolutionary inference of telomere traits among taxa may be seriously complicated by the blood cell type comprising the main source of DNA.As economic games have spread from experimental economics to other social sciences, so too have critiques of their usefulness for drawing inferences about the 'real world'. What these criticisms often miss is that games can be used to reveal individuals' private preferences in ways that observational and interview data cannot; furthermore, economic games can be designed such that they do provide insights into real-world behaviour. Here, we draw on our collective experience using economic games in field contexts to illustrate how researchers can strategically alter the framing or design of economic games to draw inferences about private-world or real-world preferences. A detailed case study from coastal Colombia provides an example of the subtleties of game design and how games can be combined fruitfully with self-report data. We close with a list of concrete recommendations for how to modify economic games to better match particular research questions and research contexts.Recent research on motion perception indicates that when we view actions in slow-motion, the perceived degree of intent behind those actions can increase. Slow-motion replays are widely used in the checking and review of refereeing decisions by video assistant referees (VAR) in association football. To test whether the decisions of referees are subject to such a bias, 80 elite English professional football officials made decisions about 60 incidents recorded in professional European leagues (recorded as fouls, yellow-card offences or red-card offences by the on-field referee). Both real-time (1×) and slow-motion (0.25×) playback speeds were used. Participants had no prior knowledge of the incidents, playback speeds or disciplinary sanctions relating to each clip. Three judgements were made about each incident extent of contact, degree of intent, and disciplinary sanction. Results showed an effect of playback speed on decision-making, but not a consistent bias due to slow-motion. Instead the distinction between yellow-card and red-card offences was clearer Under slow-motion, yellow-card incidents were judged as less severe, and red-card incidents are judged as more severe, thus enhancing the distinction between these offences. These results are inconsistent with previous scientific reports that perceived intent is heightened by slow video playback speed.China consumes more than 3.6 billion tons of coal every year. In the meanwhile, coal accounts for over 60% of the energy consumption sector. Therefore, the sustainable development of coal mines is a problem that needed to be solved by the Chinese government. During the coal resources recovery process, the protective coal pillars between the adjacent working faces lead to a vast waste of coal resources. In order to mitigate the resource-wasting issue, a new technology of roof cutting with chain arm retaining roadway was put forward in this paper. First, the procedures of retaining roadway, roof-cutting parameters and the damage ranges of roadway surrounding rock induced by roof cutting with chain arm were analysed. Selleck KPT-8602 Then, the working resistance of the temporary support equipment is given when using the new technology to retain the roadway. Next, the roof-cutting height, the temporary support equipment selection, working resistance of portal support and support parameters of the bolt and anchor cables were optimized based on the numerical calculation. The industrial experiment of retaining roadway by roof cutting with chain arm was carried out in a working face. The surrounding rock damage was lowered and controlled with the application of chain arm roof-cutting technology. Also, it was found that the variation range of the uniaxial compressive strength was only 5%, resulting in the roof damage range of 82 mm. The new technology has proved a potentially wide application in the coal mining industry with prosperous economic and safety improvement.Adult body size in holometabolus insects is directly proportional to the time spent during the larval period. The larval duration can be divided into two parts (i) pre-critical duration-time required to attain a critical size/critical weight that would result in successful completion of development and metamorphosis even under non-availability of nutrition beyond the time of attainment of critical size, and (ii) post-critical duration-the time duration from the attainment of critical size till pupation. It is of interest to decipher the relative contribution of the two larval growth phases (from the hatching of the egg to the attainment of critical size, and from the attainment of critical size to pupation) to the final adult size. Many studies using Drosophila melanogaster have shown that selecting populations for faster development results in the emergence of small adults. Some of these studies have indirectly reported the evolution of smaller critical size. Using two kinds of D. melanogaster populations, one of which is selected for faster/accelerated pre-adult development and the other their ancestral control, we demonstrate that the final adult size is determined by the time spent as larvae post the attainment of critical size despite having increased growth rate during the second larval instar.

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