Cohenraahauge7530

Whispering gallery mode (WGM) resonators demonstrate great potential for photonic and sensing applications. Yet, these devices are often disadvantaged by costly materials or complex fabrication approaches, in addition to lack of manufacturing scalability. Near-field electrospinning (NFES), a recently emerged facile fiber fabrication method, offers a solution. Here, WGM resonances are reported in Rhodamine 6G-doped poly(vinyl) alcohol (PVA) microfibers via NFES. Diameters are tuned over a range of more than 10 μm by varying substrate stage speed. Fibers display uniform distribution of dye, smooth surfaces, and circular cross-sections, all critical for supporting WGMs. High quality (Q) resonances are confirmed within fiber cross-sections through polarization experiments, free-spectral range analysis, and Mie-theory-derived mode assignment. In addition to WGMs, groups of associated spiral or conical modes are observed due to taper-induced weak optical confinement along the fiber axis. Crosslinked, dye-doped PVA fibers are utilized to sense the ethanol concentration in ethanol-water mixtures and actuation mechanisms are evaluated by comparison to theoretical spectra. The demonstration of high-Q resonances within NFES polymer microfibers is a critical step toward simple, cost effective, high-volume fabrication of WGM resonators for optoelectronics and biomedical devices.Highly flexible and stable plasmonic nanopaper comprised of silver nanocubes and cellulose nanofibres was fabricated through a self-assembly-assisted vacuum filtration method. It shows significant enhancement of the fluorescence emission with an enhancement factor of 3.6 and Raman scattering with an enhancement factor of ∼104, excellent mechanical properties with tensile strength of 62.9 MPa and Young's modulus of 690.9 ± 40 MPa, and a random distribution of Raman intensity across the whole nanopaper. The plasmonic nanopapers were encoded with multiplexed optical signals including surface plasmon resonance, fluorescence and SERS for anti-counterfeiting applications, thus increasing security levels. The surface plasmon resonance and fluorescence information is used as the first layer of security and can be easily verified by the naked eye, while the unclonable SERS mapping is used as the second layer of security and can be readily authenticated by Raman spectroscopy using a computer vision technique.We evaluated if chronic consumption of quercetin (Q) with green tea extract (GTE) enhances the bioavailability of GT polyphenols (GTPs) and reduces methylation activity as previously observed in mouse xenograft tumors. In this prospective, randomized, parallel design, placebo controlled study, thirty-one men with prostate cancer consumed daily 1 gram of GTE (830 mg of GTP) with 800 mg of Q (GT + Q) or placebo (GT + PL) for four weeks before prostatectomy. First morning voided urine was collected at baseline, 3 weeks and the day of surgery, and prostate tissue on the day of surgery. In week 3, plasma concentration of GTPs and Q was measured in blood collected before and 2 hours after the morning dose. Prostate tissue epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) were detected in 67 and 93% of participants in the GT + Q group and 75 and 94% of participants in the GT + PL group. Q was increased 14-fold, 12-fold and 4.5-fold in plasma, urine, and prostate tissue, respectively, in the GT + Q compared to the GT + PL-group. There was a trend for decreased EGC levels in urine collected prior to prostatectomy in the GT + Q compared to GT + PL-group (p = 0.053). Plasma epigallocatechin (EGC) showed a trend to increase (p = 0.066) two hours after capsule intake in the GT + Q vs. the GT + PL-group. There was no significant difference between the groups in GTP content or methylation activity in prostate tissue or RBCs. No liver toxicity was observed. Although our findings are suggestive, further studies are warranted evaluating if Q alters GTP metabolism.Zeolitic imidazolate frameworks (ZIFs) as emerging porous materials have attracted remarkable attention for their unprecedented porosity and acidic sensitive degradation that enables high drug loading and microenvironment responsive fast payload release. However, the limited functions and disadvantages of ZIFs such as early drug release, potential cytotoxicity inducing damage to major organs, and even death of animals, impede their further biomedical application. In this work, we report the first tandem post-synthetic modification of ZIF-7 with both metal ions and organic ligands. Inspired by the benzimidazole-like inhibitors that are similar to the organic ligand of ZIF-7, a chemokine (C-X-C motif) receptor 4 (CXCR4) inhibitor AMD-070 (AMD) and magnesium ions (Mn2+) were successfully tandem exchanged to the ZIF-7 framework, forming an active-targeting framework AMD-ZIF-7(Mn) for CXCR4-overexpressed esophageal squamous cell cancer. The obtained AMD-ZIF-7(Mn) showed good biocompatibility in vitro and in vivo. Meanwhile, it exhibited an excellent T1-weighted magnetic resonance imaging performance and CXCR4 targeting ability. With 5-Fu loading, AMD-ZIF-7(Mn)/5-Fu showed a synergistic therapeutic effect in DNA damage and CXCR4 inhibition of esophageal squamous cell cancer. Therefore, we propose a structural reconstruction method to effectively explore and improve the biomedical application of ZIFs in esophageal squamous cell cancer theranostics.Campylobacter jejuni is a major cause of bacterial gastroenteritis in humans that is primarily associated with the consumption of inadequately prepared poultry products, since the organism is generally thought to be asymptomatic in avian species. Unlike many other microorganisms, C. jejuni is capable of performing extensive post-translational modification (PTM) of proteins by N- and O-linked glycosylation, both of which are required for optimal chicken colonization and human virulence. The biosynthesis and attachment of N-glycans to C. jejuni proteins is encoded by the pgl (protein glycosylation) locus, with the PglB oligosaccharyltransferase (OST) enabling en bloc transfer of a heptasaccharide N-glycan from a lipid carrier in the inner membrane to proteins exposed within the periplasm. Selleck PF-02341066 Seventy-eight C. jejuni glycoproteins (represented by 134 sites of experimentally verified N-glycosylation) have now been identified, and include inner and outer membrane proteins, periplasmic proteins and lipoproteins, which are generally of poorly defined or unknown function. Despite our extensive knowledge of the targets of this apparently widespread process, we still do not fully understand the role N-glycosylation plays biologically, although several phenotypes, including wild-type stress resistance, biofilm formation, motility and chemotaxis have been related to a functional pgl system. Recent work has described enzymatic processes (nitrate reductase NapAB) and antibiotic efflux (CmeABC) as major targets requiring N-glycan attachment for optimal function, and experimental evidence also points to roles in cell binding via glycan-glycan interactions, protein complex formation and protein stability by conferring protection against host and bacterial proteolytic activity. Here we examine the biochemistry of the N-linked glycosylation system, define its currently known protein targets and discuss evidence for the structural and functional roles of this PTM in individual proteins and globally in C. jejuni pathogenesis.A total of 29 surface farmland soil samples were collected to investigate the spatial distribution and composition characteristics of 13 organophosphorus flame retardants (OPFRs), 11 polybrominated diphenyl ethers (PBDEs), and 8 novel brominated flame retardants (NBFRs) in Chengdu, China. The OPFRs were widely detected in the farmland soil with concentrations ranging from 2.92 to 160 ng g-1 dry weight (dw). BDE-209 was found with a concentration range of n.d. to 50.4 ng g-1 dw, and was the main PBDE congener accounting for 90% of ΣPBDEs in the surface farmland soil. In the case of NBFRs, only TBB and BTBPE were detected in the farmland soil from rural areas of Chengdu. There was no obvious spatial distribution of the OPFRs among different administrative regions in Chengdu (p > 0.05), but the maximum concentration of OPFRs was found in a furniture production area. Leaching experiments showed that the concentration of most of the investigated OPFRs in two kinds of soils with different mechanical compositions and TOC contents decreased with the increase of soil depth. Addition of DOM could decrease the OPFR levels in the leachate by less than 25%, with the exception of TCPP, which decreased by up to 45%. link2 More importantly, TCEP and TCPP exhibited stronger mobility than the other OPFRs in soil, and the migration capacity of TCPP was more sensitive to the DOM level, indicating that TCEP might more easily migrate from soil to groundwater in the nature.Homogeneous digital immunoassay is a powerful analytical method for highly sensitive protein biomarker detection with a simple protocol. However, it has not been multiplexed yet. In this study, we developed a multiplexed homogeneous digital immunoassay based on single-particle motion analysis (digital homogeneous non-enzyme-linked immunosorbent assay, digital Ho-Non ELISA). In this assay, multiple target antigen molecules react with the optical subpopulation of magnetic nanobeads labeled with fluorescent dyes and capture antigen-specific antibodies. Then, these beads are magnetically pulled into femtoliter-sized reactors. The surface of these reactors is modified with multiple detection antibodies specific to each antigen by molecular tethers. Each antigen on the particles reacts with the detection antibodies anchored to the surface of the reactors. Magnetic force enhances the efficiency of bead encapsulation in the reactors, and subsequent physical compartmentalization of beads enhances the binding efficiency of the antigen-antibody reaction. The tethered beads show characteristic Brownian motion distinct from free diffusion or non-specific binding of the antigen-free beads. The color of the beads is attributed to target-identification, and the number of tethered beads is attributed to the concentration of the specific target. We measured two biomarkers (PSA and IL6) as model targets by multiplexed digital Ho-Non ELISA. Our method showed higher sensitivity compared to previous digital Ho-Non ELISA and could detect multiple targets simultaneously with the same performance as in single-plex detection. link3 This new strategy has the potential to open a new avenue for next-generation multiplexed immunoassays in in vitro diagnostics.A series of trivalent Group 15 bis(tert-butylamido)cyclodiphosph(iii)azane element bi- and tricycles of the formulae [(tBuNP)2(tBuN)2]ElX, El = P, As, Sb, Bi, where X = Ph, OPh, OtBu, N3, hexamethyldisilylamide (HMDS), OTf, was synthesized from the corresponding chlorides via salt elimination. The ensuing compounds were studied spectroscopically and X-ray crystallographically with a particular focus on the length of the El-X bond. While the Group 15 element to phenyl and HMDS were of normal lengths and completely covalent, those to azide appeared to be partly ionic. The [(tBuNP)2(tBuN)2]ElI showed El-I bonds that were substantially longer than the typical element iodide bonds, suggesting a very high degree of polarity and bordering on ionic bonding. Finally, the triflate [(tBuNP)2(tBuN)2]P] + [SO3CF3]- proved to be an ion pair in the solid state. The antimony analog, however, showed a long covalent Sb-O bond in the solid state, although it appears to dissociate into ions in solution. The phosphonium triflate salt is fluxional and exhibits a previously unseen highly symmetrical structure in solution.