Cochranmcleod7632

This study attempts to investigate natural angiotensin-I converting enzyme (ACE) inhibitors. Soybean protein isolated (SPI) hydrolysate (SPIH) was prepared by Alcalase from inexpensive SPI, and their ACE inhibitory peptides were obtained via membrane separation, ethanol precipitation and adsorption chromatography enrichment. Activated carbon was more suitable for peptide enrichment than eight macroporous resins. The peptide fraction yielded under optimal conditions (protein-active carbon mass ratio 21; adsorption pH 3.0 and time 2 h; desorption time 2 h) exhibited a 10.4 times higher ACE-inhibitory activity than SPIH. Novel peptides IY, YVVF, LVF, WMY, LVLL and FF (hydrophobicity values 10.51-12.87; activity scores 0.2373-0.999) might be the main contributors to SPIH's ACE inhibition. IY had the lowest IC50 (0.53 ± 0.02 μM). YVVF had the greatest affinity (-9.8 kcal/mol) for 2OC2 (ACE's C-domain receptor) via H-bonds. IY and WMY could be potent ACE inhibitors, and their ACE-inhibitory activities unaltered and increased after in vitro gastrointestinal digestion.A LC-MS/MS method for simultaneous determination of twelve triazole enantiomers (hexaconazole, tebuconazole, triticonazole, flutriafol, diniconazole, paclobutrazol) in six fruits and vegetables was established based on a stable and self-made bridged bis(β-cyclodextrin)-bonded chiral stationary phase. Simultaneous enantio-separation of multiple analytes was achieved with resolution ca. 1.67-2.14. Magnetically assisted QuECHERS was used to simplify and optimize sample pre-treatment. The new method was validated (accuracy, precision, matrix effect, etc.). Good linearity (0.5-20 μg/L, R2 > 0.99) and high recoveries (76.1-103.4%) based on intra- and inter-day relative standard deviation (RSDs) (2.6-11.9%), were obtained. Furthermore, a total of 90 samples were analyzed using this method and enantiomeric fractions (EF) for tebuconazole in strawberry and cucumber (0.63 and 0.43, respectively) were determined as well as 0.57 for flutriafol in tomato. This high-throughput detection method supported a convenient enantiomeric monitoring for chiral pesticides in fruits and vegetables.The optimal sonication conditions (40 kHz, 80 W/L and 60 min) during Ougan juice debittering by Aspergillus niger koji extract were established. Enzymatic hydrolysis degrees of naringin and limonin were enhanced to 89.90% and 36.16%, and enzymatic hydrolysis time was shortened by 33%. Sonication significantly enhanced activities of α-l-rhamnosidases, β-glucosidases and limoninases from A. niger koji extract and facilitated break of CO bonds in naringin (p less then 0.05). These accounted for the enhanced enzymatic hydrolysis degrees and velocities of bitter compounds. Meanwhile, sonication lowered 40%, 7% and 21%, 13%, 11%, 25% of bitter, sour tastes and green, citrus-like, floral, woody notes, but enhanced 18% and 15% of fruity and sweet notes, resulting in 38% and 33% increases in over-all taste and aroma scores. Lowered levels of bitter compounds, organic acids, green, citrus-like, floral, woody aroma compounds and enhanced levels of fruity, sweet aroma compounds caused by sonication accounted for the flavor improvements.Buckwheat sprouts are rich in several nutrients such as antioxidant flavonoids that have a positive impact on human health. Although there are several studies reported the positive impact of laser light on crop plants, no studies have applied laser light to enhance the nutritive values of buckwheat sprouts. Herein, the contents of health-promoting minerals, metabolites and enzymes as well as the antioxidant and anti-inflammatory activities were determined in laser-treated (He-Ne laser, 632 nm, 5 mW) common buckwheat (CBW) and tartarybuckwheat (TBW) sprouts. Out of 49 targeted minerals, vitamins, pigments and antioxidants, more than 35 parameters were significantly increased in CBW and/or TBW sprouts by laser light treatment. Also, laser light boosted the antioxidant capacity and anti-inflammatory activities through inhibiting cyclooxygenase-2 and lipoxygenase activities, particularly in TBW sprouts. Accordingly, laser light could be recommended as a promising method to improve the nutritional and health-promoting values of buckwheat sprouts.Thioredoxins (Trxs) are important redox regulators in organisms. However, their involvement in fruit senescence and quality deterioration remains unclear. In this study, one Trx (DlTrx1) and one NADPH-dependent Trx reductase (DlNRT1) cDNAs, were cloned from longan fruit. The DlTrx1 could be effectively reduced by the DlNTR1. Expression of DlTrx1 and DlNTR1 were up-regulated during fruit senescence and quality deterioration. We further identified 33 potential Trx target proteins in longan, including one glutathione peroxidase (DlGpx). DlTrx1 could physically interact with DlGpx. DlTrx1 in combination with DlNTR1 effectively activated DlGpx activity by regulating its redox state. Cys90 in DlGPx could form a disulfide bond with either Cys42 or Cys71, which were the sites of redox modulation. Furthermore, DlGpx exhibited a higher ratio of disulfide bonds to sulfhydryl groups in senescent or deteriorative fruit. We propose that Trx-mediated redox regulation of DlGpx is involved in senescence or quality deterioration of harvested longan fruit.A ratiometric fluorescent sensor was facilely fabricated using innate fluorescence of carbendazim (MBC) and fluorescent UiO-67 to sensitively and selectively detect MBC in food matrixes. The innate fluorescence of MBC provided a signal at 311 nm (F311), and the fluorescent UiO-67 at 408 nm (F408) could recognize MBC through π-π stacking inducing fluorescent quenching relied on photoelectron transfer (PET). The ratio (F311/F408) of the fluorescence enhancement of MBC and the quenching of UiO-67 linearly responded to the MBC concentrations of 0-47.6 μmol/L with a low limit of detection (LOD) of 3.0 × 10-3 μmol/L. read more The reverse response signals of the sensor enhanced the sensitivity toward MBC and presented remarkable anti-interference capability in complex matrices. The as-prepared sensor was applied to detect MBC residues in apple, cucumber and cabbage, obtaining satisfactory accuracy and precision with the recovery of 90.82-103.45% and RSDs of lower than 3.03%.