Cappschristoffersen8547

Together, these data describe a robust human xenograft model system for investigating NSCLC brain metastases. These PDXs and cell lines show strong phenotypic and molecular correlation with the original patient tumors and provide a valuable resource for testing preclinical therapeutics.It is challenging to increase the sensitivity of a hydrogen sensor operating at room temperature due to weak sorption and tiny mass of hydrogen. In this work, an ultrasonic sensor is presented for detecting hydrogen, which is composed of a 128° YX-LiNbO3 substrate and a reduced graphene oxide (RGO) sensitive layer with a platinum catalyzer. By optimizing the depositing parameters of RGO and platinum, a considerably high sensitivity is achieved at room temperature. A frequency shift of 308.9 kHz is obtained in 100 ppm hydrogen mixed with argon, and a frequency shift of 24.4 kHz is obtained in 1000 ppm hydrogen mixed in synthetic air. It is demonstrated that in addition to strong sorption of the sensitive layer, the coaction of mass load and conductivity variation is key to high sensitivity of the sensor. By establishing the original conductivity of the sensitive layer within the "conductivity window" for enhancing electrical response, we improve the sensitivity of the ultrasonic sensor, which is available for detecting hydrogen with an extremely low concentration of 5 ppm.The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis. Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme. Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products. The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b. Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor. To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks. Our findings help to understand the inter-relationships between the enzyme structure, conformational flexibility and activity. They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.This study is focused on novel anti-corrosive support. This coating is based on the mixed matrix (MM) including epoxy and its hardener as thermoset polymer, melamine-formaldehyde as the organic phase, activated graphite as both inorganic and conductive phases, as well as wollastonite nanoparticles as filler. The wollastonite nanoparticles are synthesized by the new and novel method as suitable etching using hydrofluoric acid, "HF" at room temperature (25 ℃). The synthesized wollastonite nanoparticles are then adapted as a suitable filler during the formation of a new type of MM anti-corrosive coating for the preservation of metals form any corrosion. According to the results, during coating the MM, significant positive characteristics such as enough positive open circuit potential (OCP), small enough (icorr), low cost, significant chemical/mechanical stability and acceptable flexibility are observed. Based on to the weight-loss analysis test on the MM-modified stainless steel during a 42-day time interval, the corrosion rate % is decreased from 74.86 to 0.34. In addition, the electrochemical impedance spectroscopy reveals major enhancements in the double-layer resistance and solution resistance of the cell system. Based on the electrochemical measurements, noticeable reduction and enhancement are observed in the correction rate and potential, respectively, during introducing hard corrosive conditions such as NaCl (3.0%, w/v) and HCl (1.0 mol L-1) environments that reveal the acceptable anti-corrosive behavior of the synthesized MM. The introduced MM is therefore considered as low cost, safe, eco-friendly, industrial-justified anti-corrosion support.Aggregates of corundum crystals with skeletal to hopper morphology occur in pyroclastic rocks erupted from Cretaceous basaltic volcanoes on Mt Carmel, N. Israel. The rapid growth of the crystals trapped volumes of the parental Al2O3-supersaturated melt; phenocrysts of tistarite (Ti2O3) in the trapped melts indicate crystallization at oxygen fugacities 6-7 log units below the Iron-Wüstite buffer (fO2 = ΔIW - 6 to - 7), induced by fluxes of mantle-derived CH4-H2 fluids. Cathodoluminescence images reveal growth zoning within the individual crystals of the aggregates, related to the substitution of Ti3+ in the corundum structure. Ti contents are  2 wt%. Numerical modelling indicates that the first skeletal crystals grew in an open system, from a moving magma. Simvastatin inhibitor The subsequent linear increase in Ti reflects growth in a partially closed system, with decreasing porosity; the exponential increase in Ti close to melt pockets reflects closed-system growth, leading to dramatic increases in incompatible-element concentrations in the residual melts. We suggest that the corundum aggregates grew in melt/fluid conduits; diffusion modelling implies timescales of days to years before crystallization was terminated by explosive eruption. These processes probably operate in explosive volcanic systems in several tectonic settings.