Bushbeier1551

Organisational justice was positively correlated with levels of organisational citizenship behaviour, as was job satisfaction.

This study found that nurses in two hospitals in Egypt perceived moderate levels of organisational justice in their place of work. Nurse managers should pay extra attention to strategies that promote organisational justice among nurses.

This study found that nurses in two hospitals in Egypt perceived moderate levels of organisational justice in their place of work. Nurse managers should pay extra attention to strategies that promote organisational justice among nurses.An analytical method based on headspace gas chromatography was developed for the determination of eight organic residues in ion exchange resins, methyl isopropyl ketone, methyl butyrate, 3-pentanone, 1,3-diethyl benzene, 1,4-diethyl benzene, dichloroethane, 1,3-dichlorobenzene, and methyl methacrylate. The organic residues in different types of resins were studied to provide a basis for the safe use of ion-exchange resins in food and medicine. The main factors (chromatographic column, equilibrium temperature, equilibrium time, flow rate, etc.) that affect the accuracy and sensitivity of the eight organic residues were investigated during instrument analysis. The extraction solvent and chromatographic conditions for the samples were optimized. According to the extraction efficiencies of methyl benzene, methyl alcohol and dimethyl sulfoxide, 2.0 g of the sample was extracted with dimethyl sulfoxide under ultrasonic conditions at 20 ℃. A DB-23 chromatographic column (60 m×0.32 mm×0.25 μm) and hydrogen flame ioniof 82.3% to 109.2% at three spiked levels, and the relative standard deviation (RSD, n=6) was 1.06% to 4.16%. Eleven types of resin samples were detected by this method, and a certain amount of organic compounds were observed in the resin samples. CCT245737 The methyl methacrylate content in the methacrylate resin XAD761 was 414.4 μg/g, while that in the styrene resin LX-69B was as high as 470.8 μg/g. As opposed to traditional analytical methods, the present method has high sensitivity, good accuracy, and precision, with simple operation without derivatization or the need for acid-base treatment of the sample to reduce contamination. This method can be used to simultaneously detect a variety of organic residues in ion-exchange resins, so that the detection efficiency is significantly improved.Haloacetonitriles (HANs) are widely used in finished water as unregulated disinfection by-products. HANs may pose much threat to human health, and there is no relevant standard examination method for these compounds. A method was established for the simultaneous determination of six HANs (chloroacetonitrile (CAN), dichloroacetonitrile (DCAN), trichloroacetonitrile (TCAN), bromoacetonitrile (BAN), bromochloroacetonitrile (BCAN), and dibromoacetonitrile (DBAN)) in finished water by using purge and trap-gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The purge and trap technology helps realize automatic determination of samples after collection, without using any harmful reagent. The cost and analytical efficiency of this method were superior to those of solid phase microextraction (SPME). Considering the instability of HANs, the analysis must be carried out as soon as possible after sampling, in order to avoid significant changes in their concentration during storage. In particular, the use oinished water. The concentrations of the HANs were in the range of 0.0101-1.28 μg/L, and the total detection rate was 92.1%. The detection rates of the individual components followed the order DCAN>BCAN>CAN>TCAN>BAN>DBAN. The developed method is efficient, sensitive, and environmentally friendly. It provides a high-quality technical choice for monitoring and health risk assessment of the emerging disinfection by-products of HANs.Prednisolone is an adrenal glucocorticoid drug with immunosuppressive, anti-inflammatory, anti-allergic, and antiviral effects that are widely exploited in clinical treatment. The hydrazine residue to prednisolone directly affects medication safety and threatens the patient's health. At present, there are no relevant laws, regulations, and standards to control the residual limit of hydrazine in drugs at home or abroad. Therefore, a simple, rapid, accurate, reliable, sensitive, and selective method is urgently needed for the determination of trace hydrazine in prednisolone. Hydrazine has strong polarity and reductivity, with unstable physical and chemical properties, thus being easily oxidized. In addition, because of the lack of chromophores and low molecular weight, the detection of hydrazine is very difficult. Therefore, a derivative reagent should be introduced to reduce its polarity and generate a derivative product with a high molecular weight as well as stable physical and chemical properties. Acetone, of the determination results obtained on the same instrument by different laboratory technicians at different times was 1.77%. The durability was good, and the degree of influence of the detection results was studied by changing the chromatographic conditions. Under the original condition or conditions with initial column temperature ±5 ℃, heating rate ±2 ℃/min, or column flow rate ±0.1 mL/min, the hydrazine content in the sample solution at a spiked concentration of 6 μg/L was detected, and the RSD of the detection results was 2.58%. The established method was applied to detect hydrazine in a prednisolone standard substance procured from the market and nine batches of prednisolone samples provided by a pharmaceutical company. No hydrazine was detected in any of these samples. The established method is simple, reliable, highly sensitive, and highly selective, and it can be applied for the detection of hydrazine in prednisolone.An analytical method was established for the simultaneously determination the pentostatin and 2'-amino-2'-deoxyadenosine contents in fermentation broth by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After high-speed centrifugation, aqueous solution dilution, vortex shock, and microfiltration, the fermentation broth samples were analyzed by HPLC-MS/MS. The samples were separated on a Waters Atlantis® T3 column (100 mm×2.1 mm, 5 μm) using a gradient elution program with 10 mmol/L ammonium formate (containing 0.1% formic acid) and methanol (containing 0.02% formic acid) as the mobile phases. Moreover, a chromatographic protection column (5 mm×2.1 mm, 5 μm) was added to preserve the column efficiency. The flow rate, column temperature, and injection volume were set at 0.3 mL/min, 25 ℃, and 10 μL, respectively. Qualitative and quantitative analyses of the target compounds were performed using an ESI+ source. MS parameters such as the collision energies and tube lens offsets of penn of the pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth.Qi-Yu-San-Long decoction (QYSLD) is a classic traditional Chinese medicine prescription consisting of ten types of herbal medicines, including Astragali Radix, Polygonati Odorati Rhizoma, Scolopendra, Pheretima, Solanum nigrum L., Hedyotis diffusa Willd., Coicis Semen, Euphorbia helioscopia L., Curcumae Rhizoma, and Fritillariae Cirrhosae Bulbus, combined in a ratio of 15∶5∶3∶3∶10∶10∶10∶3∶5∶3 by weight. QYSLD has been used to treat non-small cell lung cancer (NSCLC) for over 20 years in clinical practice, and its curative effect is considered credible. However, the chemical constituents of QYSLD have not been revealed because of their complexity, which has significantly hindered the systematic clarification of the efficacy of the materials and quality evaluation. In this study, a reliable strategy based on the data-independent acquisition (DIA) technology of ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) combined with a targeted screening method was est2 terpenes, 20 amino acids, 16 phenylpropanoids, 9 organic acids, 6 sterols, 6 anthraquinones, and 15 other components. Among them, sixteen components were confirmed unambiguously with the reference substances. To better understand the chemical contribution of individual herbs to the entire decoction, the attributes of each component were summarized. This study provides a foundation for exploring the pharmacodynamic substances of QYSLD.8-Oxoguanine DNA glycosylase (OGG1) is an important enzyme that plays a key role in oxidative DNA damage repair. OGG1 can specifically recognize and excise 8-oxoG (a product of oxidative damage found in double-stranded DNA) through base excision repair (BER). OGG1 is expressed in normal tissues, and in most tumor tissues. Oxidative cellular damage can produce an inflammatory reaction, alleviating some measure of constitutive OGG1 inhibition. OGG1 inhibition in cancer cells shows some promise as a new method of cancer treatment. Most current OGG1 research focuses on regulating OGG1 with targeted small molecules. To date, no aptamer screen for OGG1 has been reported. Aptamers are single-stranded DNA (ssDNA) or RNA oligonucleotides that can bind to a target with high affinity and specificity in vitro, that can be identified by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers can be used as chemical ligands to regulate intermolecular interactions. In this study, a screen for aptamers wiacid target complexes were collected, amplified by PCR, purified, and used as an enriched secondary library in the next round of screening. High affinity aptamers were generally obtained within three rounds. Comparing results of the two screening methods, the three candidate aptamer sequences found with the highest frequency were consistent, and displayed KD values ranging from 1.71 to 2.64 μmol/L. Molecular docking analysis suggests that Apt 1 may bind to the OGG1 active pocket, which functions to repair oxidative damage. Comparison of the two screening methods indicates that one-round pressure controllable selection is more rapid and efficient, providing guidance for the design of other protein aptamer screening methods. The obtained aptamer is expected to be function effectively as an OGG1-mediated DNA repair inhibitor.Fumed silica is prepared by flame pyrolysis, where silicon halide is combusted in an oxygen-hydrogen flame, resulting in finely dispersed and thermally stable silicon dioxide. Because of its unique physical and chemical properties, including high porosity, large pore volumes, large specific area, and high chemical activity, fumed silica is widely used in rubbers, plastics, adhesives, paints, and printing inks for reinforcement, as well as in thixotropy, anti-setting, and anti-sagging applications. These functional properties of fumed silica are related to the silanol group on its surface. However, there is no accurate and convenient test method to determine the silanol group content on the surface of fumed silica. This work explores a novel method to determine the silanol group content on the surface of fumed silica by chemical reaction-headspace gas chromatography (HS-GC). Theoretically, by this method, the silanol group can rapidly react with the Grignard reagent and generate methane, the amount of which can be determined accurately by GC analysis.