Burnhamritter6208

Retinal neovascularization (RNV) is a common pathological feature in many kinds of fundus oculi diseases. Sometimes RNV can even lead to severe vision loss. Oxidative injury is one of the main predisposing factors for RNV occurrence and development. The specific mechanism may be closely related to the special structural tissues of the retina. Retinal astrocytes (RACs) are mesenchymal cells located in the retinal neuroepithelial layer. RACs have an intimate anatomical relationship with microvascular endothelial cells. They have a variety of functions, but little is known about the mechanisms by which RACs regulate the function of endothelial cells. The molecules secreted by RACs, such as exosomes, have recently received a lot of attention and may provide potential clues to address the RAC-mediated modulation of endothelial cells. In this study, we aimed to preliminarily explore the mechanisms of how RAC exosomes generated under oxidative stress are involved in the regulation of endothelial function. Our results showed that the apoptosis and autophagy levels in RACs were positively correlated with the oxidative stress level, and the exosomes generated from RACs under normal and oxidative stress conditions had different effects on the proliferation and migration of endothelial cells. However, the effect of RACs on endothelial cell function could be markedly reversed by the autophagy inhibitor 3-methyladenine or the exosome inhibitor GW4869. Therefore, oxidative stress can lead to increased autophagy in RACs and can further promote RACs to regulate endothelial cell function by releasing exosomes. © 2020 The Authors. Journal of Cellular Physiology published by Wiley Periodicals, Inc.INTRODUCTION Nurses continue to struggle to define their role as professionals in the hospital-setting often being represented in media as less competent than other health care providers. Paradoxically, an annual poll of the public consistently identifies nursing as the most trusted profession. This dichotomy of simultaneously being considered incompetent yet holding a high level of trust leads nurses to question their own professional identity. A gap exists in the literature about the professional identity of nurses who work directly with patients in the hospital environment. METHODOLOGY Therefore, the aim of this interpretive phenomenology study was to describe the lived experience of nurses working with patients in the hospital environment and the meaning of this phenomenon as it relates to their professional identity. #link# RESULTS Four themes were identified (a) being validated as an expert by providers within the healthcare system; (b) working well as a valued member of a team; (c) advocating for the patient's needs despite opposition; and (d) Valuing human-ness in the patient. IMPLICATIONS The findings provide a deeper representation of the practice of hospital-based nurses and implications for Anchornurses to be empowered in their workplace. © 2020 Wiley Periodicals, Inc.OBJECTIVE AND BACKGROUND To achieve periodontal regeneration, numerous investigations have concentrated on biomolecule supplement and optimization of bone substitute or barrier membrane. This study evaluated the benefit of combining these strategies for periodontal regeneration. METHODS Biphasic cryogel scaffold (BCS) composed of gelatin (ligament phase) and gelatin with beta-tricalcium phosphate/hydroxyapatite (BH) (bone phase) was designed as tested bone substitute, and both enamel matrix derivatives (EMD) and bone morphogenetic protein-2 (BMP-2) were applied to formulate a biomolecule-aided BCS (BBS). Functionally graded membrane (FGM) was designed as tested barrier membrane by adhering PDGF-encapsulated poly(L-lactide-co-D/L-lactide) nanofibers on the conventional membrane (CM). BBS and FGM were characterized and tested for biocompatibility in vitro. Thirty 4 × 4 × 5 mm3 periodontal intrabony defects were created on 6 Beagle dogs. Each defect was evenly assigned to one of the following treatments including BH-CM, BCS-CM, BBS-CM, BH-FGM, BCS-FGM, and BBS-FGM, for 12 weeks. The therapeutic efficiency was assessed by micro-CT and histology. RESULTS BCS and FGM sustained the release of biomolecules. The viability of MSCs was maintained in both phases of BCS and was promoted while seeding on the PDGF-encapsulated nanofibers. In CM-covered sites, BBS showed significantly greater osteogenesis (P  less then  .01) and early defect fill (P  less then  .05) relative to BH. FGM significantly promoted osteogenesis (P  less then  .05) in BH-treated sites but showed limited benefit in BBS-treated sites. On denuded roots, cementum deposition was evident in BBS-treated sites. CONCLUSIONS PDGF-loaded FGM promoted periodontal osteogenesis, and BBS with EMD-BMP-2 had potential for reconstructing alveolar ridge, periodontal ligament, and cementum. FGM and BBS combination provided limited additional benefit. © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.BACKGROUND The rhizome of ginger (Zingiber officinale Roscoe) is one of the most patronized spices worldwide and plays an important role in folklore medicine. In this study, we aimed to determine the quality of ginger samples from representative West African (Ghana, Nigeria) and East African (Uganda, Kenya) countries. By that, we also implicitly sought to determine the probable influence of location of cultivation (and the intrinsic growth conditions) on the quality of the samples. The ginger samples were pretreated by osmosonication prior to relative humidity convective drying and analyzed for differences in their metabolomes, total phenolic content (TPC) and total flavonoid content (TFC), antioxidant activities, sensory characteristics and volatile compounds composition (via electronic-nose determination). RESULTS selleck chemicals of our study showed marked source-dependent differences in the metabolomes of the samples as captured by a metabolomics approach. Based on the findings of the metabolomics study, 6-gingerol content was quantified and found to be higher in the samples of West African origin. Also, the samples from the two West African countries contained higher levels of bioactive phytochemicals as evinced by the results of TPC, TFC, e-nose analysis, and antioxidant activities. They also gave better sensory attributes. CONCLUSION In summary, for all parameters assessed, and on a country-by-country basis, the general quality trend observed was Ghana > Nigeria > Uganda > Kenya. All results taken together, our findings at least in part, point to the influence of geographical regions of cultivation on the quality of the ginger rhizomes. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry.