Akhtarkrogsgaard7476

Oral squamous cell carcinoma (OSCC) is among the leading causes of cancer-associated deaths worldwide. Family members in miR-371/372/373 miRNA cluster, which is localized at human chromosome 19q13.4, are co-expressed in both human stem cells and malignancies. The individual miRNA in this cluster are also involved in modulating the pathogenesis of malignancies as either oncogenes or suppressors. The 19q13 region is frequently gained in head and neck cancers. High expression of miR-372 and miR-373 are survival predictors for OSCC. However, the role of the miR-371/372/373 cluster in oral carcinogenesis remains to be fully investigated. We use the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 system to establish OSCC cell subclones that had the miR-371/372/373 cluster deleted. In addition, further subclones were established that had the promoter of this cluster deleted. Concordant silencing in SAS cells of miR-371/372/373 decreased oncogenic potential, increased cisplatin sensitivity, activated p53, and upregulated the expression of Bad and DKK1. We also employed the CRISPR/dCas9 synergistic activation mediator system, which allowed robust transcriptional activation of the whole miR-371/372/373 cistron. Upregulation of endogenous miR-371/372/372 expression increased both oncogenicity and drug resistance. These were accompanied by a slight activation of AKT, β-catenin, and Src. This study identifies the oncogenic role of the miR-371/372/373 cluster in OSCC. Using CRISPR based strategy can be a powerful paradigm that will provide mechanistic insights into miRNA cluster functionality, which will also likely help the development of targeting options for malignancies.This study aimed to examine the validity and reliability of the Korean version of the Work-Family Behavioral Role Conflict Scale (WFBRC-S), which was originally developed to measure work-family behavioral role conflict in American adults with a wide variety of occupations such as nurses and chief executive officers. This study used a methodological research design. The study population consisted of 235 married men and women aged 20 years or older who were living in various cities, who had been employed for three years or more. The validity of the content, construct, convergent, discriminant, and criterion related, as well as the reliability of the WFBRC-S-K, was assessed. The WFBRC-S Korean version consists of 25 items. It was found that through the validity of the composition and standards of WFBRC-S-K, it was possible to measure the conflict by focusing on behavior so that a comprehensive evaluation of the role conflict between family and work, and work and family, can be performed. Five items in the WFBRC-S-K were excluded with a standardized factor loading of less than 0.50. see more We applied the modified index to improve the model fit to build a model, it supports a good fit and reliable score for the Korean version of the WFBRC-S model. Analysis of the fit of the revised model Nomed χ2 (CIMIN/df) value of less was fit indices to 2.05 RMSEA = 0.07, RMR = 0.04, SRMR = 0.06, GFI = 0.85, IFI = 0.91, TLI = 0.90, CFI = 0.91. Criterion validity compared to the WLBOC-S showed significant correlation, and Cronbach's alpha was 0.94. Factor loadings of the 25 questions ranged from 0.49 to 0.81. The study findings confirmed the applicability of this scale for measuring the work-family behavioral role conflict in Korean adults with a wide variety of occupations. The WFBRC-S-K can be applied on the measurement of work-family conflict in nursing and other industrial sites. These results provide a foundation for future studies on work-family behavioral role conflict in Korean adult.White blood cells (WBCs) are essential components of the immune system in the human body. Various invasive and noninvasive methods to monitor the condition of the WBCs have been developed. Among them, a noninvasive method exploits an optical characteristic of WBCs in a nailfold capillary image, as they appear as visual gaps. This method is inexpensive and could possibly be implemented on a portable device. However, recent studies on this method use a manual or semimanual image segmentation, which depends on recognizable features and the intervention of experts, hindering its scalability and applicability. We address and solve this problem with proposing an automated method for detecting and counting WBCs that appear as visual gaps on nailfold capillary images. The proposed method consists of an automatic capillary segmentation method using deep learning, video stabilization, and WBC event detection algorithms. Performances of the three segmentation algorithms (manual, conventional, and deep learning) with/without video stabilization were benchmarks. Experimental results demonstrate that the proposed method improves the performance of the WBC event counting and outperforms conventional approaches.Platinum (Pt) gratings on langasite (LGS) substrates are a widely used structures in high temperature surface acoustic wave (SAW) devices. Multiple modes can be excited in Pt/LGS SAW devices owing to the heavy weight of the Pt electrode and leaky waves in the LGS substrate. In this work, we report on a detailed mode analysis of Pt/LGS SAW devices, where three commonly used LGS cuts are considered. A three-dimensional (3D) finite element method (FEM) numerical model was developed, and the simulation and experiment results were compared. The experiment and simulation results showed that there are two modes excited in the Pt/LGS SAW devices with Euler angle (0°, 138.5°, 27°) and (0°, 138.5°, 117°), which are Rayleigh-type SAW and SH-type leaky wave, respectively. Only the Rayleigh-type mode was observed in the Pt/LGS SAW devices with Euler angle (0°, 138.5°, 72°). It was found that the acoustic velocities are dependent on the wavelength, which is attributed to the change of wave penetration depth in interdigital transducers (IDTs) and the velocity dispersion can be modulated by the thickness of the Pt electrode. We also demonstrated that addition of an Al2O3 passivation layer has no effect on the wave modes, but can increase the resonant frequencies. This paper provides a better understanding of the acoustic modes of Pt/LGS SAW devices, as well as useful guidance for device design. It is believed that the Rayleigh-type SAW and SH-type leaky waves are potentially useful for dual-mode sensing applications in harsh environments, to achieve multi-parameter monitoring or temperature-compensation on a single chip.