Aggerholmhastings8404

Personalized treatment is an attractive strategy that promises increased efficacy with reduced side effects in cancer. The feasibility of such an approach has been greatly boosted by next-generation sequencing (NGS) techniques, which can return detailed information on the genome and on the transcriptome of each patient's tumor, thus highlighting biomarkers of response or druggable targets that may differ from case to case. However, while the number of cancers sequenced is growing exponentially, much fewer cases are amenable to a molecularly-guided treatment outside of clinical trials to date. In multiple myeloma, genomic analysis shows a variety of gene mutations, aneuploidies, segmental copy-number changes, translocations that are extremely heterogeneous, and more numerous than other hematological malignancies. Currently, in routine clinical practice we employ reduced FISH panels that only capture three high-risk features as part of the R-ISS. On the contrary, recent advances have suggested that extending ge close and conventional genetic testing will not be able to return the required information. This will mandate that even in routine practice NGS should soon be adopted owing to a higher informative potential with increasing clinical benefits. Copyright © 2020 Bolli, Genuardi, Ziccheddu, Martello, Oliva and Terragna.Digestive system neuroendocrine carcinomas (NECs) are rare neoplasms originating from neuroendocrine cells with a poor prognosis and limited effective treatments. Programmed cell death protein 1/ligand 1 (PD-1/PD-L1) blockade has been used in the management of more than 10 solid tumors and has achieved promising clinical outcomes. PD-L1 expression, immune cell infiltration, tumor mutational burden (TMB), and microsatellite instability (MSI) are all verified biomarkers that can predict the response to anti-PD-1/PD-L1 therapy. Here, we investigated PD-L1 expression and immune cell infiltration density by immunohistochemical (IHC) staining of tumor samples from 33 patients with digestive system NECs. Tumor and paratumor normal samples from 31 of these patients underwent whole-exome sequencing to evaluate TMB and the MSI-high (MSI-H) status. In total, 29.0% of digestive system NECs had positive PD-L1 expression according to the tumor proportion score (TPS). Infiltration of CD3+, CD8+, and CD68+ cells was observed in 69.7, 27.3, and 54.5% of patients, respectively. The TMB value for patients sequenced ranged from 0.57 to 11.75 mutations/Mb, with a median of 5.68 mutations/Mb. mSINGS, MSIsensor, and MSIseq were used to analyze the MSI status according to the sequencing data, and in our evaluation, no MSI-H status was detected. Our data might indicate a limited potential of anti-PD-1/PD-L1 monotherapy in digestive system NECs, although clinical trials are warranted. Copyright © 2020 Xing, Ying, Li, Gao, Sun, Li, Bai, Cheng and Wu.Ultrasound pretreatment with different times (0, 4, 8, 10 min) on olive paste previous malaxation of olive oil extraction along with depitting and water application procedures were studied. The effects of these procedures on oil yield, quality parameters and oxidative stability have been studied. Therefore, the olive oil yield increase with increasing time of ultrasound treatment. On the other hand, the application of ultrasound did not adversely affect the quality characteristics, as well as the antioxidant activity, when comparing with untreated paste. Furthermore, the oxidative stability data, we can conclude that ultrasound treatment can affect the olive oil oxidative stability. This study could provide useful information for industry to produce olive oil with high yield and quality. © 2020 Published by Elsevier B.V.This method paper explains the i-Tree Cool River model algorithms for simulating the response of river water temperature to urban greening. The model captures the warming and cooling impacts of urban development and restoration through a water and energy budget. The water budget includes river inflows from urban storm sewers and reservoirs, and the associated water temperatures. The energy budget adjusts radiation fluxes due to riparian shading and evapotranspiration, and propagates temperature downstream. Restorative cooling of the river can be simulated through algorithms for cool groundwater, either as direct inflows or by river water replacement called hyporheic exchange. Novel features in the model include diurnal variation in riparian shading, use of the Army Corps of Engineers HEC-RAS model predicted river depths and velocities, and periodic boundary conditions to rapidly extend restoration scenarios.•Freely available code in C++ for Visual Studio, with a detailed manual and sample inputs and outputs at http//www.itreetools.org/research_suite/coolriver.•Useful for simulating river warming or cooling due to urban development or greening.•Well documented source code compatible with requirements of other modeling groups. © 2020 The Author(s). Published by Elsevier B.V.The analysis of environmental microplastic particles using FTIR microscopy is a challenging task, due to the very high number of individual particles within a single sample. Therefore, automatable, fast and robust approaches are highly requested. OTSSP167 supplier Micro particles were commonly enriched on filters, and sub- or the whole filter area was investigated, which took more than 20h and produced millions of data, which had to be evaluated. This paper presents a new approach of such filter area analysis using an intelligent algorithm to measure only those spots on a filter that would produce evaluable FTIR data. Empty spaces or IR absorbers like carbon black particles were not measured which successfully reduced the total analysis time from 50h to 7h. The presented method is based on system independent Python workflow and can easily be implemented on other FTIR systems. •Fast and intelligent FTIR microscopy area mapping without FPA detector•Total time reduction from 50 h to 7 h•Platform independent approach based on Python. © 2019 Published by Elsevier B.V.Introduction Spontaneous ureteric rupture is an extremely rare cause of acute abdominal pain in the intrapartum and postpartum period. We present the case of a right ureteric rupture diagnosed immediately postpartum. Case A 23-year-old woman in her second pregnancy (who had had a previous caesarean section) developed acute-onset right-flank pain 12 h after vaginal delivery. A contrast computerized tomography scan suggested a ureteric injury; ureteroscopy diagnosed a proximal ureteric rupture and a stent was placed. Discussion This case outlines an extremely rare cause of abdominal pain in the peripartum. There can be serious complications, including urinoma, abscess and sepsis, and therefore the diagnosis should not be delayed. © 2020 The Authors.