Griffithcurtis2806

BACKGROUND Isoindole-1,3(2H)-dione derivatives are known to have cytotoxic effects on many cancer cells. The anticancer activity of these compounds varies depending on the substituents attached to them. Therefore, the effect of substituents is very important when determining the anticancer activities of molecules. We have recently reported an example of the substituent effect. According to that work, the anticancer activity against HeLa, C6, and A549 cancer cell lines of isoindole-1,3(2H)-dione compounds containing tert-butyldiphenylsilyl ether, azido, and hydroxyl groups was examined by our group. It was found that an isoindole-1,3(2H)-dione compound containing both tert-butyldiphenylsilyl ether group and azido groups showed higher anticancer activity than 5-fluorouracil and another isoindole-1,3(2H)-dione compound containing both azido and hydroxyl groups. After we discovered that tert-butyldiphenylsilyl ether group in the skeletal structure of isoindole-1,3(2H)-dione exhibits anticancer activity against Heeir anticancer activities or other biological properties. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND In cancer cells, re-activation of Epithelial-Mesenchymal Transition (EMT) program through Discoidin Domain Receptor1 (DDR1) lead to metastasis. DDR1-targeted therapy with siRNA might be a promising strategy for EMT inhibition. Therefore, the aim of this study was to investigate the effect of DDR1 knockdown in the EMT, migration, and apoptosis of prostate cancer cells. For this purpose, the expression of DDR1 down regulated by the siRNA approach in LNcap-FGC and DU145 prostate cancer cells. METHODS Immunocytochemistry carried out to assessment of EMT. E-cadherin, N-cadherin, Bax, Bcl2, and the phosphorylation level of proline-rich tyrosine kinase 2 (Pyk2) and Map Kinase Kinase 7 (MKK7) was determined using the western blot. Wound healing assay used to evaluate Cell migration. Flow cytometry employed to determine the apoptosis rate in siRNA-transfected cancer cells. https://www.selleckchem.com/products/lazertinib-yh25448-gns-1480.html RESULTS Our findings showed that stimulation of DDR1 with collagen-I caused increased phosphorylation of Pyk2 and MKK7 signaling molecules that led to the induction of EMT and migration in DU-145 and LNcap-FGC cells. In contrast, DDR1 knockdown led to significant attenuation of EMT, migration, and phosphorylation level of Pyk2 and MKK7. Moreover, DDR1 knockdown via induction of Bax expression and suppression of Bcl-2 expression induces apoptosis. CONCLUSION Collectively, our results indicate that the DDR1 targeting with siRNA may be beneficial for inhibition of EMT and induction of apoptosis in prostate cancer. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.Drug discovery in the scope of cancer therapy has been focused on conventional agents that nonselectively induce DNA damage or selectively inhibit the activity of key oncogenic molecules without affecting their protein levels. An emerging therapeutic strategy that garnered attention in recent years is the induction of Targeted Protein Degradation (TPD) of cellular targets by hijacking the intracellular proteolysis machinery. This novel approach offers several advantages over conventional inhibitors and introduces a paradigm shift in several pharmacologic aspects of drug therapy. While TPD has been found to be the major mode of action of clinically approved anticancer agents such as fulvestrant and thalidomide, recent years have witnessed systematic endeavors to expand the repertoire of proteins amenable to therapeutic ablation by TPD. Such endeavors have led to three major classes of agents that induce protein degradation including molecular glues, Proteolysis Targeting Chimeras (PROTACs) and Hydrophobic Tag (HyT)-based degraders. Here, we briefly highlight agents in these classes and key advances made in the field with a focus on clinical translation in cancer therapy. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Cancer refers to a collection of diseases where cells begin to multiply uncontrollably. Breast cancer is the most predominant malignancy in women. Herbal medicine is one of the important health care system in most of developing countries. Many studies have shown that naturally occurring compounds may support the prevention and treatment of various diseases, including cancer. Some of the plant extracts and isolated compounds shown photosensitizing activities and reduces cell proliferation whereas some revealed photoprotective effects. OBJECTIVES The biological properties and medicinal uses of extracts and bioactive compounds from V. nilgiriensis have not been investigated. This study aims to evaluate the cytotoxic effects of V. nilgiriensis in combination with 680 nm laser irradiation on MCF-7 breast cancer cells. METHODS The inverted microscopy, ATP and LDH assay were used to analyze the cellular morphology, proliferation, cytotoxicity after the treatment with V. nilgiriensis bark extract. The diodsis can be considered as a potent therapeutic agent for the treatment of cancer. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.OBJECTIVE The main aim of the present work is to synthesize chloramphenicol impurity A (CLRM-IMP-A) in the purest form and its subsequent characterization by using a panel of sophisticated analytical techniques (LCMS, DSC, TGA, NMR, FTIR, HPLC and CHNS) to provide as reference standard mentioned in most of the international compendiums including IP, BP, USP, and EP. The present synthetic procedure has not been disclosed anywhere in the prior art. METHOD A simple, cheaper and new synthesis method was described for the preparation of CLRM-IMP-A. It was synthesized and characterized by FTIR, DSC, TGA, NMR (1H and 13C), LC-MS, CHNS, and HPLC. RESULTS CLRM-IMP-A present in drugs and dosage form can alter the therapeutic effects and adverse reaction of a drug considerably, it is mandatory to have a precise method for the estimation of impurities to safeguard of the public health. Under these circumstances, the presence of CLRM-IMP-A in chloramphenicol (CLRM) requires strict quality control to satisfy the specified regulatory limit.