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Moreover, we also demonstrate that although the biomacromolecules such as proteins couldn't directly tune crystallinity and morphology of ZIF-90, they can perform as a stabilizer for anchoring metal ion to prevent crystal fast transformation of ZIF-90 during synthetic process.Artificial bone grafts possess the advantages of good biodegradability, customizable dimensions, and sufficient mechanical properties, which can promote cell proliferation and differentiation in bone tissue regeneration. 3D printing is a delicate approach that endows the scaffolds with excellent controllability and repeatability when compared with conventional bio-fabrication methods. However, the limitation of printing resolution somehow makes it difficult to prepare bone defect substitution with high porosity and hierarchical construct. In this study, we utilized polylactic acid (PLA) as printing materials and developed a smart strategy to combine 3D printing technology with bio-fabrication methods. find more A porous planar scaffold was printed and then rolled up into a spiral structure with adjustable pore size and porosity. The topographic features and morphology of the artificial scaffolds were examined through stereomicroscope and SEM, respectively. The porous spiral scaffold presented good mechanical properties in a set of mechanical testing. Later, the human fetal osteoblasts (hFOB) were cultured on the porous spiral scaffold and its control groups for a total of 28 days. The MTS analysis, alkaline phosphatase (ALP) assay, and alizarin red S (ARS) staining were used to analyze the cell proliferation, osteogenic differentiation, and mineral deposition after a certain period of time. The results indicated that compared with the other two scaffolds, the porous spiral scaffold with larger surface area and better interconnections between internal porous networks could significantly improve the spatial cell compartment and promote cell growth and differentiation. The porous spiral scaffold may see versatile applications in large-volume bone defects regeneration.It is significant to develop a protective film (coating) for touch screen panels with combined absorbing UV and antibacterial performances. This work developed a smart strategy to fabricate multifunctional protective films for touch screen panels. Polymeric microgels of polyquaternium-10 (PQ) and sodium alginate (SA) were firstly fabricated based on electrostatic interactions. The PQ-SA microgels solution was then cast on a glass substrate to produce PQ-SA films. Further, peroxo titanic acid as cross-linking agent was employed to cross link PQ-SA films via forming coordination bonds between polymers and Ti of peroxo titanic acid, whereafter TiO2 nanoparticles were generated in situ in PQ-SA films by hydrolyzing the peroxo titanic acid to endow PQ-SA films with excellent absorbing UV ability. Moreover, water-soluble quercetin and resveratrol nanoparticles are fabricated by incorporating solvent exchange method and borate esterification reaction and were loaded into the PQ-TiO2-SA films to give the films good antibacterial performance. The as-prepared multifunctional films not only exhibited excellent absorbing UV, filtering visible light and antibacterial properties, but also possessed good water resistance, abrasion resistance, self-healing performances and high adhesion to a glass substrate. We believe that present multifunctional film as a protective film for touch screen panels can provide protection for users.This work was done to systematically elucidate the corrosion behavior of austenitic stainless steel subjected to various degree of cold deformation (10 %, 20 % & 30 %). The experiments were performed in phosphate buffer saline (PBS) solution having different concentrations of bovine serum albumin (0.2, 0.5, 1.0, 2.0, 4.0 g L-1). Potentiodynamic polarization tests and electrochemical impedance spectroscopy (EIS) analysis were performed to obtain the corrosion parameters. Scanning electron microscopy with energy dispersive X-ray (SEM-EDX), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) were used to determine the surface morphologies and chemical compositions of the surface films. Contact angle analysis was also used to detect the hydrophilic character of sample surfaces. The BSA had a considerable effect of inhibition on the corrosion of SSs in annealed as well as in deformed state due to its adsorption on surface of steel. For annealed samples, at 4.0 g L-1BSA concentration, the corrosion resistance was drastically decreased but interestingly not for sample with more than 10 % deformation and the concentration effect of BSA is also not very significant after 0.5 g L-1 for deformed surfaces. The breakdown potential for 30 % deformed sample is quite higher in presence of BSA even at 4.0 g L-1 while it is lowest for annealed samples in the same condition. The variation in contact angle with deformation is very less after adsorption of BSA. On the basis of the obtained results, mechanism aspect for corrosion of steel in presence of protein is also deliberated.A facile and novel colorimetric method for screening of α-glucosidase inhibitors (AGIs) from flavonoids using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic probe is proposed. This method is based on the colorimetric detection of ascorbic acid (AA) through the TMB oxidation reaction catalyzed by horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2). In the TMB/H2O2/HRP system, HRP catalyzes the oxidation of H2O2 to ‧OH radical which oxidizes TMB to blue-colored oxidized TMB (oxTMB). In the presence of AA, the production of ‧OH radical is suppressed and causes the decrease of oxTMB, resulting in the fading of the blue color and the decrease of absorbance at 652 nm. Based on this, the existence of AA can be facilely identified. In the 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2 G)/α-glucosidase (α-Glu) system, the produced AA inhibits the oxidation of TMB to blue-colored oxTMB. In the presence of AGIs, the production of AA is inhibited, which inhibits the reduction of oxTMB, resulting in a blue color recovery and an increase of the absorbance at 652 nm. Based on this, the colorimetric method is developed for screening of AGIs from 7 flavonoids.