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It also decreased the Bax/Bcl-2 ratio and the expressions of cytochrome c, cleaved caspase-9, cleaved caspase-3, and cleaved PARP. In addition, it suppressed the phosphorylation of the p38 mitogen-activated protein kinase (MAPK) pathway. CONCLUSIONS UA attenuates oxidative stress-induced apoptosis via inhibiting the mitochondrial-related apoptosis pathway and modulating the p38 MAPK pathway, suggesting that it may be an effective neuroprotective agent. ©2020 The Korean Nutrition Society and the Korean Society of Community Nutrition.Microcystins produced by cyanobacteria pose a great threat to human health by releasing toxins upon cell death. In the present study, we studied microcystin production in the cyanobacterial strains Anabaena cylindrica (B629 and 2949) and Fremyella diplosiphon (SF33) exposed to 1, 2 and 4 g/L sodium chloride (NaCl). Cultures grown for 7 days in BG11/HEPES medium were pelleted, re-grown in the corresponding NaCl levels, and enzyme linked immunosorbent assay (ELISA) performed. ELISA assays revealed enhanced microcystin production in A. cylindrica B629 exposed to 4 g/L NaCl and A. cylindrica 29414 exposed to 2 and 4 g/L NaCl, after growth in the corresponding NaCl levels for 14 days. We observed a significant decrease (p >0.05) in microcystin levels in the control strains after exposure to NaCl for 5 days. After exposure to 1, 2, or 4 g/L NaCl for 10 days, no microcystin release was observed in A. cylindrica B629, A. cylindrica 29414 or F. diplosiphon SF33. Sodium dodecyl sulfate polyacrylamide gel electrophoresis identified the presence of an additional band at 120 - 130 kDa in A. cylindrica B629 exposed to 2 and 4 g/L NaCl, and at 14 kDa in cultures amended with 1 and 2 g/L NaCl as well as the untreated control, indicating that exposure to salinity induces alterations in protein expression.Background There is increasing evidence indicating an incidence of infertility and also the risk of endometrial cancers among smokers. However, the mechanism underlying nicotine adverse effect on female reproduction remains unclear. Growing evidence has suggested that environmental exposures such as nicotine could modulate the epigenome. No study has yet been published to evaluate the direct effect of nicotine on the epigenome profiling of human endometrial stromal cells (HESC). Herein, we decided to examine the direct effects of nicotine on global genomic DNA methylation status and DNA methyl- transferases (DNMTs) gene expression in HESC. HESC were treated with different doses of nicotine (0 or control, 10- 11, 10- 8 and 10- 6) M for 24 h and their genomic global DNA methylation and gene expression of DNMTs (DNMT1, DNMT3A, and DNMT3B) were investigated using ELISA and real-time PCR, respectively. Results Nicotine treatments reduced the average level of DNMTs gene expression by 90, 79, and 73.4% in 10- 11, 10- 8 and 10- 6 M of nicotine treated cells as compared to control cells, respectively (p  less then  0.05). selleck products Also, 10- 8 and 10- 6 M of nicotine concentrations effectively reduced the amounts of 5-methylated cytosine (5-mC) by 1.09 and 1.87% compared to control cells, respectively (p  less then  0.05). The 5-mC percentages were positively correlated with the relative cellular DNMTs expression in HESC as verified by the Pearson correlation test. Conclusion An interesting possibility raised by the current study is that the reduced genomic global DNA methylation level in HESC may be partly due to the suppression of DNMTs gene expression caused by nicotine in these cells. Graphical abstract © The Author(s) 2020.Background Modern testing paradigms seek to apply human-relevant cell culture models and integrate data from multiple test systems to accurately inform potential hazards and modes of action for chemical toxicology. In genetic toxicology, the use of metabolically competent human hepatocyte cell culture models provides clear advantages over other more commonly used cell lines that require the use of external metabolic activation systems, such as rat liver S9. HepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal for toxicity testing. We assessed the performance of the flow cytometry in vitro micronucleus (MN) test and the TGx-DDI transcriptomic biomarker to detect DNA damage-inducing (DDI) chemicals in human HepaRG™ cells after a 3-day repeat exposure. The biomarker, developed for use in human TK6 cells, is a panel of 64 genes that accurately classifies chemicals as DDI or non-DDI. Herein, the TGx-DDI e field of genetic toxicology. It provides mechanistic insight in a human-relevant cell-model, paired with measurement of a conventional endpoint, to inform the potential for adverse health effects. This work provides support for combining these assays in an integrated test strategy for accurate, higher throughput genetic toxicology testing in this metabolically competent human progenitor cell line. © The Author(s) 2020.It has yet to be determined whether or not the probability of developing cancer due to radiation exposure levels of low doses is proportional to the dose. Herein, for radiation hormesis occurring at low doses, mathematical models using functions that take a mountain-like shape having two inflection points are considered. The following perspectives were obtained (i) When the probability of developing cancer decreases at radiation levels above the natural background dose, the radiation hormesis effect occurs up to ~ 12.4 mSv. (ii) When there is a proportional relationship at ≥750 mSv, the radiation hormesis effect occurs up to ~ 225 mSv. Thus, by performing studies at the molecular and cellular levels for radiation doses at ≤16.8 or 307 mSv, it is possible to investigate carcinogenesis resulting from low radiation doses. © The Author(s) 2020.A 73-year-old man was admitted with sudden onset of dyspnea. Contrast-enhanced computed tomography showed acute pulmonary thromboembolism and deep vein thrombosis. He was started on the direct oral anticoagulant rivaroxaban (factor Xa inhibitor) and this resolved the thrombus. Serological analysis revealed that his risk of thrombosis was primary antiphospholipid syndrome (APS). He has remained free of recurrent venous thromboembolism (VTE) for two years while under rivaroxaban. We present a case with VTE due to APS for whom direct oral anticoagulant was effective. . © 2019 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.