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A T-maze test is an experimental approach that is used in congenital research. However, the food reward-based protocol for the T-maze test in fish has low efficiency and a long training period. MTX-211 EGFR inhibitor The aim of this study is to facilitate the T-maze conditions by using a combination of the principles of passive avoidance and a spatial memory test. In our modified T-maze settings, electric shock punishment (1-2 V, 0.3-0.5 mA) is given at the left arm, with a green cue at the right arm. Also, the depth of both arms of the T-maze was increased. The parameters measured in our T-maze design were latency, freezing time, and time spent in different areas of the T-maze. We validated the utility of our modified T-maze protocol by showing the consistent finding of memory impairment in ZnCl2-treated fish, which has been previously detected with the passive avoidance test. In addition, we also tested the spatial memory performance of leptin a (lepa) mutants which displayed an obesity phenotype. The results showed that although the learning and memory performance for lepa KO fish were similar to control fish, they displayed a higher freezing behavior during the training phase. In conclusion, we have established a modified T-maze protocol that can be used to evaluate the anxiety, learning, and memory capacity of adult zebrafish within three days, for the first time.To further improve the precision and efficiency of structural health monitoring technology and the theory of large-scale structures, full-field non-contact structural geometry morphology monitoring is expected to be a breakthrough technology in structural safety state monitoring and digital twins, owing to its economic, credible, high frequency, and holographic advantages. This study validates a proposed holographic visual sensor and algorithms in a computer-vision-based full-field non-contact displacement and vibration measurement. Using an automatic camera patrol experimental device, original segmental dynamic and static video monitoring data of a model bridge under various damage/activities were collected. According to the temporal and spatial characteristics of the series data, the holographic geometric morphology tracking algorithm was introduced. Additionally, the feature points set of the structural holography geometry and the holography feature contours were established. Experimental results show that the holographic visual sensor and the proposed algorithms can extract an accurate holographic full-field displacement signal, and factually and sensitively accomplish vibration measurement, while accurately reflecting the real change in structural properties under various damage/action conditions. The proposed method can serve as a foundation for further research on digital twins for large-scale structures, structural condition assessment, and intelligent damage identification.The treatment of traumatic brain injury (TBI) remains a challenge due to limited knowledge about the mechanisms underlying neuronal regeneration. This current study compared the expression of WNT genes during regeneration of injured cortical neurons. Recombinant WNT3A showed positive effect in promoting neuronal regeneration via in vitro, ex vivo, and in vivo TBI models. Intranasal administration of WNT3A protein to TBI mice increased the number of NeuN+ neurons without affecting GFAP+ glial cells, compared to control mice, as well as retained motor function based on functional behavior analysis. Our findings demonstrated that WNT3A, 8A, 9B, and 10A promote regeneration of injured cortical neurons. Among these WNTs, WNT3A showed the most promising regenerative potential in vivo, ex vivo, and in vitro.Capacitive MEMS accelerometers with area-variable periodic-electrode displacementtransducers found wide applications in disaster monitoring, resource exploration and inertialnavigation. The bonding-induced warpage, due to the difference in the coefficients of thermalexpansion of the bonded slices, has a negative influence on the precise control of the interelectrodespacing that is essential to the sensitivity of accelerometers. In this work, we propose the theory,simulation and experiment of a method that can alleviate both the stress and the warpage byapplying different bonding temperature on the bonded slices. A quasi-zero warpage is achievedexperimentally, proving the feasibility of the method. As a benefit of the flat surface, the spacing ofthe capacitive displacement transducer can be precisely controlled, improving the self-noise of theaccelerometer to 6 ng/√Hz @0.07 Hz, which is about two times lower than that of the accelerometerusing a uniform-temperature bonding process.Few studies assess dog ownership and walking with both self-reported or perceived and audited or objective walkability and physical activity measures. Across two years, we examined both types of walkability and activity measures for residents living within 2km of a "complete street"-one renovated with light rails, bike lanes, and sidewalks. Audited walkability (Irvine-Minnesota Inventory) was more consistently related to dog ownership and walking groups than perceived walkability (Neighborhood Environment Walkability Scale-Abbreviated). Self-reported leisure walking was much higher (289-383 min per week) among dog walkers than among other groups (100-270 min per week), despite no difference in accelerometer-measured light or moderate-to-vigorous physical activity (MVPA). Furthermore, the most powerful difference between groups involved single-family detached home residence, which was much lower among non-dog-owners (44%) than among non-dog-walkers or dog walkers (81% and 70%, respectively). Given discrepancies across walkability and activity measures, we recommend future use of walkability audits and objectively measured physical activity over the current emphasis on self-report measures. We also urge greater attention to increased densities of housing, which may negatively affect dog ownership levels unless compensating supports for dog ownership and walking are created by public health messaging, dog-friendly policies, and dog-friendly housing and community design.