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168, SNPs p = 0.166, and DNPs p = 0.177), while a significant increase was seen with all other concentrations (p ˂ 0.05). Ra values of ZNP and DNP groups were significantly higher than those of the SNPs group (p less then 0.001). The addition of any of the fillers to acrylic denture base materials significantly increased the hardness (p ˂ 0.05), with ZNPs and DNPs having values lower than those of the SNPs group (p less then 0.001). CONCLUSIONS Although nanofiller addition increased the hardness of denture base material, Ra was adversely affected when the concentration exceeded 0.5%. Therefore, 0.5% is suggested to be the most appropriate ratio to improve hardness with acceptable Ra . © 2020 by the American College of Prosthodontists.BACKGROUND In recent years, the relationship between malignant tumors and platelets has been paid more attention. The increase of platelets is an independent risk factor for the poor prognosis of some malignant tumors. METHODS This study retrospectively analyzed the clinical and pathological data of 114 patients with initial gastric cancer from August 2005 to August 2018 in Shandong Provincial Hospital. Single-factor and multifactor survival analysis were used to evaluate the effect of platelet elevation on postoperative survival. The gastric cancer tissues of the Jinan Central Hospital and its matched paracancerous tissues were collected. Selleck Nimodipine The expression of platelets in tissues was detected by immunofluorescence technique. Different numbers of platelets were co-cultured with MKN-45 cells, CCK-8 assay and transwell assay were performed, and the expression of epithelial-mesenchymal transition-related proteins was detected. RESULTS Platelet count was independent factors affecting prognosis. The stratified analysis showed that there was a statistically significant difference in the 5-year survival rate between the platelet-increase group and the normal platelet group in the TNM stages I-II. The expression of platelets in gastric cancer tissues was higher than that in adjacent tissues. The results of CCK-8 and transwell showed that platelets significantly enhanced the proliferation and metastasis capability of MKN-45 cells in a concentration-dependent manner. After co-culture, the expression level of E-cadherin protein in MKN-45 cells decreased, and the protein expression levels of N-cadherin, vimentin, and VEGFA increased. CONCLUSION Platelet elevation is closely related to the occurrence, development, and metastasis of gastric cancer, and platelet count can be used as a prognostic indicator for malignant tumors. © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc.PURPOSE Adipose derived mesenchymal stem cells (MSCs) are hypothesized to supplement tissues with growth factors essential for regeneration and neovascularization. The purpose of this study was to determine the effect of MSCs with respect to neoangiogenesis when seeded onto a decellularized nerve allograft in a rat sciatic nerve defect model. METHODS Allograft nerves were harvested from Sprague-Dawley rats and decellularized. MSCs were obtained from Lewis rats. 10 mm sciatic nerve defects in Lewis rats were reconstructed with reversed autograft nerves, decellularized allografts, decellularized allografts seeded with undifferentiated MSC or decellularized allografts seeded with differentiated MSCs. At 16 weeks, the vascular surface area and volume were evaluated. RESULTS The vascular surface area in normal nerves (34.9 ± 5.7%), autografts (29.5 ± 8.7%), allografts seeded with differentiated (38.9 ± 7.0%) and undifferentiated MSCs (29.2 ± 3.4%) did not significantly differ from each other. Unseeded allografts (21.2 ± 6.2%) had a significantly lower vascular surface area percentage than normal nonoperated nerves (13.7%, p = .001) and allografts seeded with differentiated MSCs (17.8%, p = .001). Although the vascular surface area was significantly correlated to the vascular volume (r = .416; p = .008), no significant differences were found between groups concerning vascular volumes. The vascularization pattern in allografts seeded with MSCs consisted of an extensive nonaligned network of microvessels with a centripetal pattern, while the vessels in autografts and normal nerves were more longitudinally aligned with longitudinal inosculation patterns. CONCLUSIONS Neoangiogenesis of decellularized allograft nerves was enhanced by stem cell seeding, in particular by differentiated MSCs. The pattern of vascularization was different between decellularized allograft nerves seeded with MSCs compared to autograft nerves. © 2020 Wiley Periodicals, Inc.PURPOSE The "babysitter" procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross-nerve graft. First, an end-to-side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the "babysitter" procedure and find its efficacy in rats with facial nerve complete paralysis. MATERIALS AND METHODS A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two-stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery. RESULTS Facial nucleus stained with retrograde tracers proved the re-innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross-facial nerve graft group (p  less then  .05). Histological examination found a significant difference in myelin g-ratio between two groups (p  less then  .05). CONCLUSION This study investigated the "babysitter" procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy. © 2020 Wiley Periodicals, Inc.