Mcgrathennis5525
Procalcitonin (PCT) is an acute phase response protein, which can be used as an indicator for early diagnosis of infection. At present, the main detection methods for PCT are electrochemiluminescence and enzyme-linked immunofluorescence. We aimed to explore the accuracy of PCT determination in a domestic chemiluminescence detection system and its correlation with other systems.
Clinical specimens were collected, and the precision, linearity, biological reference interval, contamination rate, Clinical reportable scope, and methodological comparison of the determination of PCT in a Chinese chemiluminescence detection system were evaluated and preliminarily verified by referring to Clinical and Laboratory Standards Institute (CLSI) documents or industry standards.
The results of precision verification showed that the coefficient of variation (CV) values of the variation coefficient of precision in the samples of low and high values were 2.07% and 0.83% respectively, while the CV values of the total variatidological comparison showed that the correlation coefficient between the Mindray CL900I and Roche E602 was 0.9986, while the correlation coefficient between the Mindray CL900I and the Snibe 2000 was 0.983. The test results were consistent with the experimental requirements.
The domestic chemiluminescence detection system has a good performance in the determination of calcitonin, as indicated by the measures of precision, linearity, biological reference interval, carrying contamination rate, and Clinical reportable scope, and can thus be used for clinical specimen detection. The results of methodological comparison showed that the correlation coefficient between the Mindray CL900I and Roche E602 was 0.9986, while the correlation coefficient between the Mindray CL900I and the Snibe 2000 was 0.983. The test results were consistent with the experimental requirements.
To explore the performance status of Chinese postgraduate medical students in literature searching.
A self-designed online questionnaire was used to assess the literature search performance of postgraduate students (PGSs) from the classes of 2016, 2017, 2018, and 2019 from two medical colleges. The items of the questionnaire mainly included the demographic characteristics of the PGSs, methods of literature review, literature reading habits, and use of literature. We also designed a self-assessed score that ranged from the lowest 1 point to the highest 5 points.
A total of 902 PGSs (482 male, average age 29.4±5.8 years old, working time range 0-10 years, average 3.7±2.4 years) completed the questionnaire. Most PGSs investigated literature only at the work tasks (632, 70.1%) and writing papers (571, 63.3%) stages. Of the PGSs, 542 (60.1%) PGSs searched literature frequency (≥1 paper/week), and 114 (12.6%) did not perform advanced searches, and some had no knowledge of advanced search techniques at all. Most PGSs had not read more than 100 Chinese articles or English articles before. Most PGSs were used to read articles from the most authoritative journals (665, 73.7%) or high impact factor (IF) (540, 59.9%). PGSs (845, 93.7%) only read the full text of articles they deemed important. Of the PGSs, 441 (48.9%) did not use literature management tools. For self-assessed score of literature searching and reading skills, the mean was 2.1 (standard deviation, 0.8). Reading literature efficiently (710, 78.7%) and tracking recent literatures (615, 68.2%) were the two needed literature skills reported.
Chinese medical PGSs still have room for improvement in relation to literature investigation. Intensive training in literature searching should be given to improve their performance.
Chinese medical PGSs still have room for improvement in relation to literature investigation. Intensive training in literature searching should be given to improve their performance.
Bacterial infection is one of the most common causes of sepsis, with acute lung injury (ALI) being a related complication. Pterostilbene (PTS) is extracted from blueberries, peanuts, and grapes, and has numerous pharmacologic activities. The aim of the present study was to explore the underlying role of PTS protects against sepsis-mediated ALI.
We established a sepsis model induced by cecal ligation and puncture (CLP) in rats. TWS119 The rats were randomly divided into five groups (n=5 each) sham group, CLP group, Dexmedetomidine group (Dex, 50 µg/kg) and PTS groups (25 and 50 mg/kg). Twenty-hours hours after CLP, PTS was intraperitoneally injected for 14 continuous days. The rats were killed, and blood and lung tissue were collected for pathological analysis and mRNA and protein detection.
Our findings showed that PTS reduced the wet/dry ratio and ameliorated sepsis-induced pulmonary fibrosis (PF), which was associated with improvement of pathological damage in lung tissues. We also observed the inhibitory effect of PTS on apoptosis and release of inflammatory cytokines (i.e., tumor necrosis factor-α, interleukin-6, and monocyte chemotactic protein 1). In addition, PTS markedly suppressed the phosphorylation levels of Janus kinase-2 (JAK2) and signal transducer and activator of transcription 3 (STAT3).
Our results indicated that PTS inhibited the PF, apoptosis, and inflammatory response via the JAK2/STAT3 pathway in a sepsis-induced ALI rat model, providing a candidate for drug therapy of sepsis-induced ALI.
Our results indicated that PTS inhibited the PF, apoptosis, and inflammatory response via the JAK2/STAT3 pathway in a sepsis-induced ALI rat model, providing a candidate for drug therapy of sepsis-induced ALI.
Retinoblastoma is a rare cancer of the retina that accounts for 3% of all childhood cancers. The aim of this study was to illuminate the oncogenic role and potential molecular mechanisms of the microRNA miR-154-5p and autophagy-related gene 7 (ATG7) in retinoblastoma, and to establish a nude mouse model in order to explore new therapeutic horizons for the disease.
Quantitative reverse transcription-polymerase chain reaction and western blot were performed to detect the expression levels of miR-154-5p and ATG7. The targeting relationship between miR-154-5p and ATG7 was analyzed by employing the luciferase reporter assay. MiR-154-5p mimic and pcDNA-ATG7 were transfected, either alone or in combination, into Y79 cells. The subsequent
experiments involved four groups the control group, miR-154-5p group, ATG7 group, and miR-154-5p + ATG7 group. Orthotopic xenograft models were established by injecting BALB/c athymic nude mice with treated and untreated Y79 cells.
Y79 cells were transfected with miR-NC or miR-154-5p.
