Carsondaugherty4497

FPAP was used for early diagnosis of Dengue fever and provided an 8-fold higher sensitivity while the limit of detection (LOD) was recorded to be in femto-gram per milliliter range which is significantly low when compared to other existing techniques or conventional assay. This platform allows different types of paper/fiber bio-receptive platforms to be incorporated within the design that promises simultaneous recognition of multiple infectious agents. We obtained a range of PLGA-based composites containing sol-gel bioactive glasses (SBG) from the SiO2-CaO and SiO2-CaO-P2O5 systems. Eight SBGs with different CaO/SiO2 ratios with and without P2O5 were incorporated at 50% w/w to PLGA matrix and structured into thin films suitable for cell culture. The SBG/PLGA composites were examined for their bioactivity in simulated body fluid (SBF), ion release profile in culture media with and without cells, and osteoinductivity in standard human bone marrow stromal cell (hBMSC) cultures without osteogenic growth factors. Our results indicate different surface activity of composites depending on the presence/absence of P2O5 in SBG composition. Furthermore, ion release profile to culture medium differed depending on the presence/absence of cells. Direct culture of hBMSC on the SiO2-CaO/PLGA composite films resulted in elevated Runx-2 mRNA, opposite to low Runx-2 mRNA levels on SiO2-CaO-P2O5/PLGA films. All studied composites increased Osx mRNA levels. Whereas some of SiO2-CaO/PLGA composites did not elevate BMP-2 and -6 proteins in hBMSC cultures, high levels of these BMPs were present in all cultures on SiO2-CaO-P2O5/PLGA composites. All composites induced BMP-related Tak1 signalling, whereas Smad1 signalling was restricted mostly to composites containing three-component SBGs. ALP activity of hBMSC and BMP-related luciferase activity of mouse BRITE cells differed depending on whether the cells were stimulated with culture medium conditioned with SBG/PLGA composites or the cells were directly cultured on the composite surfaces. Altogether, beyond bioactivity and osteoinductivity of SBG/PLGA composites, our studies show key differences in the biological response to both the bioactive material dissolution products and upon direct cell-material contacts. TMP269 Polyglycolic acid (PGA) is a faster biodegradable polymer for various implants, frequently causing different macrophages' activation. In this study, we undertook a comparable study of PGA's degradation on macrophages' activation with different PGA crystallinity (in porous and fibrous 3D scaffolding format) in an in vitro and in vivo model. The incubation medium containing PGA degradation products, with different pH value of 7.1, 6.1 and 3.6, was added to RAW 264.7 macrophages' culture to simulate different degradation phases. The addition of hydrochloric acid with the same pH values in the culture media was used to compare and simplify the acid types' effect on macrophages. The scaffolds were implanted to mouse subcutaneously for 6 weeks. To correlate the degradation rate between the in vitro and in vivo models, PGA scaffolds were grafted by rhodamine-b covalently enabling the detection of PGA degradation through fluorescence intensity decay. It was confirmed that porous PGA degraded faster than fibrous scaffolds due to lower crystallinity. The acidic PGA degradation products (GA) did not promote IL-10 production, but inhibited IL-1β, IL-6 and TNF-α production in 7-days' culture significantly. The use of HCl with the same pH value as PGA degradation products in culture did not produce the same inhibition effect as GA. The mouse model showed that the degradation of PGA scaffolds was accelerated in vivo in the first two weeks, mainly due to tissue ingrowth. The fast degradation of porous scaffolds triggered M1 macrophages into the implantation site, whilst the slow degradation of PGA fibers promoted the polarization of macrophages into M2 pro-healing phenotypes. This study provides a good foundation to study and design biodegradable biomaterials toward immunomodulation. V.Coating of amorphous calcium phosphate (ACP) on titanium (Ti) implants is a promising technique for enhancing bone-forming ability because of its dissolution in vivo. Surgical site infection is one of the serious complications associated with implant devices. In order to achieve both the antibacterial properties and bone-forming ability on the surface of Ti implants, Ag-doped ACP coating films were fabricated. The ACP coating film is expected to work as a carrier of Ag. Ta was added to suppress the dissolution rate of the Ag-ACP coating films, which expands its potential applications. Ag and Ta co-doped ACP coating films were fabricated on Ti substrates by radiofrequency (RF) magnetron sputtering. The sputtering targets were hot-pressed sintered compacts with the same Ag concentration of 10 mol% and varying Ta concentration (0, 0.8, and 8.0 mol%), while the RF power was changed from 8 to 50 W. With increasing RF power, Ag concentration in the coating films decreased. The fabricated ACP coating films were dense and smooth, with their constituent elements (P, Ca, Ag, and Ta) distributed homogeneously along the depth direction. In addition, Ag existed as ions in the ACP regardless of Ta concentration. We clarified for the first time that the dissolution of Ag-containing ACP coating films in solution was suppressed by a Ta addition. Antibacterial activity was obtained from the release of Ag+ ions through continuous dissolution of Ag and Ta co-doped ACP coating films. Here, we report the application of mussel-inspired surface coating to prepare heparin-mimetic biomacromolecules modified magnetic Fe3O4 nanoparticles as recyclable anticoagulant. Sodium alginate sulfate (SAS), which has the similar chemical structure and bioactivity with heparin, was synthesized at first. Dopamine (DA) was then grafted onto the backbone of SAS as the mussel-inspired adhesive macromolecule (DA-g-SAS), followed by being coated onto Fe3O4 nanoparticles. The SAS coated Fe3O4 nanoparticles could combine both the advantages of magnetic responsiveness and blood compatibility. The measurements revealed that the modified nanoparticles showed improved anticoagulant property as well as good recyclable property. The study provides a promising method to introduce nanomaterials into the field of hemodialysis. And the heparin-mimic polysaccharide biomacromolecules modified Fe3O4 nanoparticles can be considered as attractive material for potential application for hemodialysis.