Mygindmarker5094

Taken together, these results indicated that TaMpc1-D4 negatively modulated drought tolerance by regulating the capacity of the enzyme system and the expression of stress-related and antioxidant-related genes.Abiotic stresses threaten the productivity and quality of economically important perennial fruit crops such as apple (Malus × domestica Borkh.). WRKY transcription factors play various roles in plant responses to abiotic stress, but little is known regarding WRKY genes in apple. Here, we carried out functional characterization of an apple Group IIa WRKY gene (MdWRKY30). qRT-PCR analysis found that MdWRKY30 expression was induced by salt and drought stress. A subcellular localization assay showed that MdWRKY30 is localized to the nucleus. A transactivation assay found that MdWRKY30 has no transcriptional activation activity. A Y2H assay indicated that MdWRKY26, MdWRKY28, and MdWRKY30 interact with each other to form heterodimers and homodimers. Transgenic analysis revealed that the overexpression of MdWRKY30 in Arabidopsis enhanced salt and osmotic tolerance in the seedling stage, as well as during the seed germination and greening cotyledon stages. MdWRKY30 overexpression enhanced tolerance to salt and osmotic stresses in transgenic apple callus through transcriptional regulation of stress-related genes. Together, our results demonstrate that MdWRKY30 is an important regulator of salinity and osmotic stress tolerance in apple.Protein S-nitrosylation, which refers to the redox-based posttranslational modification of a cysteine thiol by the attachment of a nitric oxide (NO) group, modulates a variety of enzyme activities. Monodehydroascorbate reductase (MDHAR) is essential for ascorbic acid (AsA) regeneration, which protects plant cells against damage by detoxifying reactive oxygen species (ROS). However, the relationship between S-nitrosylation and the role of tomato MDHAR (SlMDHAR) under salt stress remains unclear. In this paper, we show that the SlMDHAR mRNA expression, enzyme activity, protein level, total S-nitrosylated proteins and S-nitrosylated SlMDHAR protein level in tomato leaves significantly increase after NaCl treatment. To further evaluate the function of SlMDHAR under salt stress, overexpressed transgenic tobacco plants were used. The germination rate and root length of the overexpressed plants under NaCl stress were significantly higher than those of wild-type (WT) plants. Meanwhile, the transgenic plants had lower ROS accumulation, higher antioxidant enzyme activities and AsA-DHA ratio, more proline and soluble sugar contents than those in WT plants under salt stress. With a higher expression of stress-related genes, the transgenic plants demonstrated lower Na+ and higher K+ accumulation compared with WT plants. The NO accumulation and S-nitrosylated MDHAR level were higher in transgenic plants than in WT plants after NaCl treatment. In contrast, virus-induced gene silencing (VIGS) of SlMDHAR tomato plants showed enhanced sensitivity to salt stress and have lower S-nitrosylated MDHAR protein. These results suggested that SlMDHAR confers salt stress tolerance by alleviating oxidative damage probably involving the S-nitrosylation of MDHAR.Barley (Hordeum vulgare) is one of the most important crops in the world, ranking 4th in the worldwide production. Crop breeders are facing increasing environmental obstacles in the field, such as drought, salinity but also toxic over fertilization which not only impacts quality of the grain but also an yield. One of the most prevalent mechanisms of gene expression regulation in plants is microRNA-mediated silencing of target genes. We identified 13 barley microRNAs and 2 microRNAs* that are nitrogen excess responsive. Four microRNAs respond only in root, eight microRNAs only in shoot and one displays broad response in roots and shoots. We demonstrate that 2 microRNAs* are induced in barley shoot by nitrogen excess. For all microRNAs we identified putative target genes and confirmed microRNA-guided cleavage sites for ten out of thirteen mRNAs. None of the identified microRNAs or their target genes is known as nitrogen excess responsive. Analysis of expression pattern of thirteen target mRNAs and their cognate microRNAs showed expected correlations of their levels. OSI-027 molecular weight The plant microRNAs analyzed are also known to respond to nitrogen deprivation and exhibit the opposite expression pattern when nitrogen excess/deficiency conditions are compared. Thus, they can be regarded as metabolic sensors of the regulation of nitrogen homeostasis in plants.RNA helicases are omnipresent plant proteins across all kingdoms and have been demonstrated to play an essential role in all cellular processes involving nucleic acids. Currently, these proteins emerged as a new tool for plant molecular biologists to modulate plant stress responses. Here, we review the crucial role of RNA helicases triggered by biotic, abiotic, and multiple stress conditions. In this review, the emphasis has been given on the role of these proteins upon viral stress. Further, we have explored RNA helicase mediated regulation of RNA metabolism, starting from ribosome biogenesis to its decay upon stress induction. We also highlighted the cross-talk between RNA helicase, phytohormones, and ROS. Different overexpression and transgenic studies have been provided in the text to indicate the stress tolerance abilities of these proteins.Cork oak (Quercus suber L.) is a species of ecological, social and economic importance in the Mediterranean region. Given its xerophytic adaptability, the study of cork oak's response to drought stress conditions may provide important data in the global scenario of climate change. The mechanisms behind cork oak's adaptation to drought conditions can inform the design and development of tools to better manage this species under the changing climate patterns. Metabolomics is one of the most promising omics layers to capture a snapshot of a particular physiological state and to identify putative biomarkers of stress tolerance. Drastic changes were observed in the leaf metabolome of Q. suber between the different experimental conditions, namely at the beginning of the drought stress treatment, after one month under drought and post rehydration. All experimental treatments were analyzed through sPLS to inspect for global changes and stress and rehydration responses were analyzed independently for specific alterations.