Mannriley6514

Results Our in vivo and in vitro results indicated that TQ significantly mitigates brain damage and motor dysfunction after ischemic stroke. These observations coincided with curtailed cell death, inflammation, oxidative stress, apoptosis, and autophagy. Most importantly, Nrf2/HO-1 signaling pathway activation by TQ was vital in the modulation of the above processes. Lastly, we found TQ to have minimal toxicity in liver tissue.Conclusion Our study gives credence to TQ as a promising intervention therapy for cerebral ischemia that decreases inflammation, oxidative stress, and neuronal cell death via the Nrf2/HO-1 pathway, along with modulation of apoptotic and autophagic processes.

Symbols are commonly used to represent information of health care products included multipurpose solutions (MPSs) for contact lens (CL) care.

Little knowledge is available about recognition and comprehension by regular soft CL wearers of commonly used symbols in the instructions of CL MPSs marketed worldwide.

CL wearers from the UK and Spain were invited to answer an anonymous on-line questionnaire (distributed though Facebook and Twitter) concerning the recognition and comprehension of seven common symbols (ISO 152232017 and EN 9802008) included in MPS package instructions.

Answers of one hundred-ten soft CL wearers who use MPS (27 in the UK and 83 in Spain) were analysed showing low recognition and comprehension of the surveyed symbols even in wearers with >5years of CL wear (71.8%). The CE marked was the most recognised (96% in the UK and 88% in Spain, P =0.29) and understood (78% in the UK and 85% in Spain, P =0.25) symbol, but other important symbols, such as 'Caution' and 'Do not reuse', wereS in soft CLs care.

species are prolific sources of bioactive secondary metabolites known especially for their antimicrobial and anticancer activities.

This study sought to isolate and characterize antioxidant molecules biosynthesized by

sp. KTM18. The antioxidant potential of an isolated compound and its toxicity were accessed.

The compound was purified using bioassay-guided chromatography techniques. Nuclear magnetic resonance (NMR) experiments were carried out for structure elucidation. The antioxidant potential of the isolated compound was determined using DPPH free radical scavenging assay. The toxicity of the isolated compound was measured using a brine shrimp lethality (BSL) assay.

Ethyl acetate extract of

sp. KTM18 showed more than 90% inhibition of DPPH free radical at 50µg/mL of the test concentration. These data were the strongest among 13

isolates (KTM12-KTM24). The active molecule was isolated and characterized as maculosin (molecular formula, C

H

N

O

as determined by the [M + H]

peak at 261.1259). The DPPH free radical scavenging activity of pure maculosin was higher (IC

, 2.16 ± 0.05µg/mL) than that of commercial butylated hydroxyanisole (BHA) (IC

, 4.8 ± 0.05µg/mL). No toxicity was observed for maculosin (LD

, <128µg/mL) in brine shrimp lethality assay (BSLA) up to the compound's antioxidant activity (IC

) concentration range. The commercial standard, berberine chloride, showed toxicity in BSLA with an LD

value of 8.63 ± 0.15µg/mL.

Maculosin may be a leading drug candidate in various cosmetic and therapeutic applications owing to its strong antioxidant and non-toxic properties.

Maculosin may be a leading drug candidate in various cosmetic and therapeutic applications owing to its strong antioxidant and non-toxic properties.Skeletal muscle also plays a vital role in regulating the movement energy storage and health of metabolism. In order to investigate the expression profile of protein and phosphor-proteins in chicken skeletal muscle during embryonic development, we performed phosphor-proteomics analysis by label-free and TiO2 enrichment strategy in chicken leg muscle tissues of at embryonic age embryo day 7(E7), E12, E17 and 3-day post-hatch (D3). The study led to the identification of 4332 proteins in the proteome and 1043 phosphorylation modification sites in the phosphorylated proteome, corresponding to 718 proteins (FC ≥ 2 or FC ≤ 0.5 and p  less then  0.05). The DEP-associated biological processes were involved in Focal adhesion, Glycolysis/gluconeogenesis, Arginine and proline metabolism by KEGG analysis. PPI analyses revealed that these DEPs TNNC1, TNNC2, TNNT2, TNNT3 and phosphorylated DEPs MYLPF interacted with involved pathways. Integrative analysis of proteome and phosphoproteome data found 324 common proteins, corresponding to 521 modification sites and Focal adhesion was the only pathway significantly enriched. These results provide a basis for further understanding the proteome and phosphoproteome and their regulatory biochemical pathways during the development of embryonic chicken skeletal muscle.Bamboo shoot is an indispensable vegetable in Southeastern China, so a survey of heavy metals in bamboo shoots is a relevant topic of interest. GANT61 The present study sought to analyse the content of seven heavy metals (As, Cd, Cr, Cu, Mn, Pb and Zn) in six bamboo shoot species from Southeastern China. Percentages of 3.8% (Cd) and 8.9% (Pb) of the samples exceeded the maximum limits as established by the Chinese legislation. Further health risk assessment exhibited that the Cd intake contribution of these samples could not be negligible. The hazard index (HI) at average exposure was less than one. The average HI values in moso bamboo shoot were significantly higher than those of other bamboo shoot species. Overall, the results indicated that the intake of bamboo shoots is safe for consumers in Southeastern China.

Acute brain ischemia is accompanied by a disruption of low-molecular-weight aminothiols (LMWTs) homeostasis, such as homocysteine (Hcy), cysteine (Cys), and glutathione (GSH). We investigated the redox balance of LMWTs in blood plasma and its influence on ischemic stroke severity and the functional outcome in patients with an acute period.

A total of 177 patients were examined. Total and reduced forms of LMWTs were determined in the first 10-24 h. Stroke severity and functional state were estimated using the National Institutes of Health Stroke Scale (NIHSS) and the modified Rankin Scale (mRs) at admission and after 21 days.

Patients with high levels of total Hcy (> 19 μM) showed significantly reduced redox statuses of all LMWTs. Patients with low total GSH levels (≤ 1.07 μM) were at an increased risk of higher stroke severity (NIHSS > 10) compared to patients with a total GSH level > 2.64 μM (age/gender-adjusted odds ratio 4.69, 95% CI 1.43-15.4).

(1) low total GSH level can be considered as a novel risk marker for the severity of acute stroke in conditions of low redox status of LMWTs and (2) high Hcy levels associated with low redox status of LMWTs.