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Irradiation of dentinal tubules using diode laser could be effective for routine clinical treatment of dentinal hypersensitivity compared to NaF and GC tooth mousse.

Irradiation of dentinal tubules using diode laser could be effective for routine clinical treatment of dentinal hypersensitivity compared to NaF and GC tooth mousse.

The aim was (1) to study the cytotoxicity of novel Bioactive Caries-detecting Dye solution (BCD) and its antimicrobial activity against

,

,

, and

and (2) comparative assessment of BCD and Carie-Care for efficient removal of caries (stereomicroscope) and dentin tubule occlusion (scanning electron microscope [SEM]).

For BCD cytotoxic study (direct contact method), colorimetric MTT assay, and cell line study(L929 mouse fibroblast NCTC clone 929 strain L) was performed. Xenetix 350, chitosan, nanohydroxyapatite (nHA), BCD, and Carie-Care solutions were subjected to the antimicrobial activity through blood agar well diffusion method, and the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. On 20 extracted human carious teeth a comparative pilot study was done for BCD (Group A,

= 10) and Carie-Care (Group B,

= 10), and evaluated visually and radiographically. After mechanical excavation of caries with a spoon excavator, teeth sectioned longitudinally and stereomicroscopically were evaluated (8x-40x) by two observers. The percentage of dentinal tubule occlusion was evaluated with SEM for both solutions. Statistical kappa analysis of agreement was 0.7-0.8 (

< 0.01). Mann-Whitney test ranks and Wilcoxon signed-rank test (

= 0.01) were applied.

Cytotoxicity test revealed BCD to be nontoxic and biocompatible. Antimicrobial tests (zone of inhibition) showed BCD > chitosan > chlorhexidine > Carie-Care > Xenetix 350 > nHA. MIC and MBC values suggested chlorhexidine > BCD > Carie-Care. Stereomicroscopic analysis showed effective mechanical removal of caries in BCD without residual dye in the dentinal tubules as compared to Carie-Care. Dentinal tubule occlusion (SEM analysis) was 80%-85% for BCD and 10% for Carie-Care.

Profound synergistic effect for BCD was observed with advantage of radiographic assessment.

Profound synergistic effect for BCD was observed with advantage of radiographic assessment.

Irrigants remove or dissolve smear layer formed during instrumentation. check details Thus it is important to study the effect of different irrigant solutions on smear layer removal.

The aim of this study is to determine which irrigant effectively removes the smear layer from the coronal, middle, and apical third of the root canal.

One hundred and twenty single-rooted mandibular premolars were decoronated and biomechanical preparation was done through hand instrumentation up to size 40 k file with 2.5% NaOCl irrigation between each successive filing, followed by irrigation with 5 ml of saline. The teeth were divided into Groups I, II, III, and IV containing 30 samples each and irrigated with 5 ml of 17% ethylenediamine tetraacetic acid (EDTA), 10% Citric acid, 7% Maleic acid and normal saline respectively for 1 min and final irrigation was done with 5 ml of distilled water of each sample. The canals were dried with 2% absorbent paper points. The roots were then split with a chisel and mallet. One-half of each tooth wcal third. However, in apical third 7% maleic acid is the most efficacious irrigant in smear layer removal.

Bonding of composite with enamel and dentin gets compromised if the tooth is restored with composite immediately post bleaching. The application of antioxidants has shown to have a positive effect on it.

The aim of this study was to evaluate and compare the effect of guava seed extract solutions in various concentrations on the shear bond strength (SBS) of composite resin to bleached enamel when applied for 5 min, 10 min, and 120 min.

Fifty-five maxillary incisors were procured, and labial surfaces of 50 specimens were bleached with 37.5% hydrogen peroxide. These specimens were divided into 3 experimental groups Group 1-3 comprising 15 specimens each (

= 15), and the remaining specimens were divided into two groups Group 4 (positive control group) and Group 5 (negative control group). Groups 1-3 were further divided into 3 subgroups according to the application period of antioxidant subgroups A, B, and C. Specimens were stored in distilled water for 24 h. SBS testing was done using the universal testing machine. Data were tabulated and subjected to statistical analysis using a three-way analysis of variance with

Tukey's test.

Guava seed extract showed a complete reversal of the compromised bond strength, and promising results were seen with increased concentration.

The use of antioxidants effectively reversed the compromised bond strength of bleached enamel, and an increase in the concentration of antioxidants reduces the duration of its application.

The use of antioxidants effectively reversed the compromised bond strength of bleached enamel, and an increase in the concentration of antioxidants reduces the duration of its application.

The present study aimed to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA)-Angelus, Biodentine™, and EndoSequence cement in furcation perforations using protein leakage assessment.

The present study was conducted using seventy extracted human maxillary and mandibular molars with intact furcation. The samples were randomly allocated into three groups (

= 20) based on repair material used Group 1 - MTA-Angelus, Group 2 - Biodentine™, and Group 3 - EndoSequence. Two additional groups served as positive and negative controls (

= 5). Using the leakage assessment apparatus, the presence of protein was detected with a reagent (Coomassive Brilliant Blue) every day for 60 days. One-way ANOVA and

Tukey's test were used for statistical analysis using SPSS software.

All the groups exhibited protein leakage from day 1. Biodentine showed minimum (0.1201 mg/ml), MTA showed maximum (0.3738 mg/ml), and EndoSequence had intermediate (0.2465 mg/ml) leakage. None in the negative control and all of the positive control specimens leaked during the experimental period of 60 days.

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