Haugebuch2590

Consumption of game in Germany has increased during the past 10 years. Wild boar (Sus scrofa), roe deer (Capreolus capreolus) and red deer (Cervus elaphus) are the most frequently hunted and consumed game animals in Germany, yet information on the occurrence of zoonotic pathogens in these animal species is scarce. To better estimate the public health risk emanating from handling and consumption of game, this study investigated seroprevalences of Toxoplasma gondii in game hunted in the German federal state of Brandenburg during two hunting seasons from 2017 to 2019. Toxoplasma gondii-specific antibodies were detected in 24.4% (44/180, 95% CI 18.4%-31.4%) of wild boar, 12.8% (16/125, 95% CI 7.5%-20%) of roe deer and 6.4% (3/47, 95% CI 1.3%-17.5%) of red deer using a commercial ELISA kit. Seroprevalences were similar in the two hunting seasons. Correlation between sex and seropositivity could not be observed. A rise in seroprevalence was seen with increasing age in all studied game species. Observed seroprevalences suggest that T. gondii is endemic in the sylvatic environment in the German federal state of Brandenburg and imply that game could represent a relevant source for human T. gondii infection. © 2020 The Authors. Zoonoses and Public Health published by Blackwell Verlag GmbH.Since its outbreak in December 2019 in China, the novel coronavirus disease (COVID-19) has rapidly spread and affected several countries. It has resulted in a difficult situation for cancer patients owing to the risks of the epidemic situation outbreak as well as cancer. Patients with cancer are more likely than the general population to contract COVID-19 because of the systemic immunosuppressive status caused by malignant diseases or anticancer treatment. Lung cancer has the highest morbidity and mortality in China and the world. Most patients with lung cancer are smokers with poor underlying lung conditions and low immunity, thus it is vital to protect them from epidemic diseases during cancer treatment. It is necessary to provide individualized medical treatment and management of treatment-related adverse events for patients with lung cancer based on patients' conditions and regional epidemic patterns. © 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.Bacteria are known to communicate with each other through signalling molecules that regulate gene expression within the population. However, the way in which hydrophobic signals are released and transmitted among bacterial population is not well understood. Recent studies show that membrane vesicles (MVs) are involved in delivering hydrophobic signals, such as in N-hexadecanoyl-L -homoserine lactone (C16-HSL) signalling in Paracoccus denitrificans Pd1222. In this study, we identified the AHLs produced in P. aminophilus JCM7686, P. aminovorans NBRC16711, P. thiocyanatus JCM20756, P. versutus JCM20754 and P. yeei ATCC BAA-599, and show that the main AHL produced in all the strains is C16-HSL. Our results show that these Paracoccus species also release MVs that carry C16-HSL, but at different proportions. Most of the strains carry C16-HSL in MVs, while in P. aminovorans NBRC16711, very little C16-HSL was detected in MVs, but was found in other fractions of the supernatant. Given the utilisation of a common signal, we showed that these Paracoccus species can share signals with P. denitrificans Pd1222, and examined the role of MVs in signalling. Our study provides new insights into the way in which bacteria communicate using hydrophobic signals. This article is protected by copyright. All rights reserved. This article is protected by copyright. IACS-13909 mw All rights reserved.Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1-deficient mice share some phenotypic traits with Fgf23- or Klotho-deficient mouse models. Here, we tested whether Memo1 gene expression is regulated by calciotropic hormones or by changing the dietary mineral load. MLO-Y4 osteocyte-like cells were cultured and treated with 1,25(OH)2 -vitamin D3 . Wild-type C57BL/6N mice underwent treatments with 1,25(OH)2 -vitamin D3 , parathyroid hormone, 17β-estradiol or vehicle. Other cohorts of C57BL/6N mice were fed diets varying in calcium or phosphate content. Expression of Memo1 and control genes was assessed by qPCR. 1,25(OH)2 -vitamin D3 caused an acute decrease in Memo1 transcript levels in vitro, but not in vivo. None of the hormones tested had an influence on Memo1 transcripts, whereas the assessed control genes reacted the expected way. Dietary interventions with calcium and phosphate did not affect Memo1 transcripts but altered the chosen control genes' expression. We observed that Memo1 was not regulated by calciotropic hormones or change in mineral load, suggesting major differences between the regulation and physiological roles of Klotho, Fgf23, and Memo1. © 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.A 74-year-old man presented for surgical treatment to alleviate chronic post-herniorrhaphy inguinal pain. Physical and imaging examinations suggested that his pain was due to his ilioinguinal nerve being entrapped by a meshoma composed of bilayer mesh and plug mesh. The patient strongly desired mesh removal, although it appeared challenging because of adhesion of the meshes from the previous herniorrhaphies. Anticipating technical difficulty, we performed laparoscopic totally extraperitoneal repair followed by open mesh removal. Thus, the risk of damaging the peritoneum and visceral organs during open mesh removal was eliminated because the peritoneum had already been separated from the pathogenic mesh during the laparoscopic repair. The patient's chronic pain was drastically relieved. Combination surgery may therefore be a safe and useful technique in select patients with chronic postoperative inguinal pain. This approach could also prevent hernia recurrence. © 2020 Japan Society for Endoscopic Surgery, Asia Endosurgery Task Force and John Wiley & Sons Australia, Ltd.