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Leukemia constitutes just 3% of all malignancies but because of its high incidence and mortality in children and persons below 40 years of age, it is considered one of the devastating malignant conditions. This study was undertaken to evaluate the anticancer effects of Friedelin triterpenoid against the human AML-196leukemia cells.

CCK-8 assay was used to determine cell viability. DAPI staining was used for the assessment of apoptosis. Annexin V/propidium iodide (PI) analysis was employed for the detection of percentage of apoptosis. Transwell assays were used for cell migration and invasion. Western blotting was used for the determination of protein expression.

The results showed Friedelin inhibited the proliferation of the human AML-196 cells with little effects on the normal cells. Investigation of the underlying mechanisms showed that Friedelin induced apoptosis in AML-196 cells. Friedelin-induced apoptosis was linked with upregulation of cleaved caspase-3, 8 and 9, as well as cleaved PARP. The Bax protein levels were increased and of Bcl-2 were decreased. Transwell assays showed that Friedelin suppressed the migration and invasion of the AML-196leukemia cells. Additionally, Friedelin also blocked the MEK/ERK and PI3K/AT signalling cascades dose-dependently.

Taken together, Friedelin may prove beneficial in the treatment of leukemia.

Taken together, Friedelin may prove beneficial in the treatment of leukemia.

The development of erratic distribution of cervical metastases from oral cavity squamous cell carcinoma (OSCC) bypassing the typical metastatic pattern can possibly challenge the role of the classic neck dissection. The purpose of this study was to assess the role of lymphoscintigraphy (LS) and radio-guided neck dissection as a simple and widely accessible method with a favorable cost/benefit ratio, able to improve the OSCC staging and possibly to tailor the surgical approach to cervical lymph node dissection.

From June 2015 to December 2018, 16 patients (5 women, 11 men, median age 59.5±12.5 years) with cN0 (10) and cN+ (6) OSCC were enrolled. The day before surgery all patients underwent LS with acquisition of planar and SPECT (Single Photon Emission Computed Tomography)/CT images, after a peritumoral injection of 99mTc-Nanocoll® (median 74±1.2 MBq). Patients underwent tumor excision and a radioguided neck dissection, using a portable gamma camera. The sentinel lymph nodes (SLNs) were isolated and separately analyzed in 200-micron sections and pancytokeratin immunohistochemistry assessment, looking for micrometastases.

A homolateral lymphatic spread on LS was observed in all cases, whereas in 5 cases (31.3%) lymphatic drainage was contralateral to the OSCC site. In one cN0 patient, a skip micrometastasis has been identified in a SLN.

The results of the present study may suggest a role of LS and radioguided neck dissection in detecting the real lymphatic spread of OSCC, in order to improve the oncological assessment and to tailor the neck dissection.

The results of the present study may suggest a role of LS and radioguided neck dissection in detecting the real lymphatic spread of OSCC, in order to improve the oncological assessment and to tailor the neck dissection.

MicroRNA-215 (miR-215) has been reported to show different effects in human cancers. However, the function of miR-215 remains unclear in nasopharyngeal carcinoma (NPC). Hence, this research was designed to investigate the effect of miR-215 on the development of NPC.

The expression levels of miR-215 and RB1 were examined in NPC via the qRT-PCR assay. The protein expression was observed through immunocytochemical assay and western blot. MTT (methyl thiazolyl tetrazolium) and Transwell assays were employed to explore the effect of miR-215. The relationship between miR-215 and retinoblastoma (RB)1 was assessed by dual luciferase assay.

Upregulation of miR-215 was identified in NPC tissues and predicted worse prognosis of NPC. Cell proliferation, migration and invasion were promoted by overexpression of miR-215 in NPC. Furthermore, miR-215 directly targeted RB1 which was downregulated in NPC. MiR-215 promoted the progression of NPC through targeting RB1. In particular, miR-215 promoted EMT (epithelial-mesenchymal transition) and activated Wnt/β-catenin pathway in NPC.

MiR-215 promoted the development of NPC through suppressing RB1 and activating Wnt/β-catenin pathway.

MiR-215 promoted the development of NPC through suppressing RB1 and activating Wnt/β-catenin pathway.

Osteosarcoma (OS) is the primary malignant tumor which is common in children and adolescents. selleck chemicals The treatment effect is still poor, though the treatment strategy has been dramatically improved.

Differentially expressed genes in metastatic osteosarcoma and non-metastatic osteosarcoma were obtained first. Secondly, co-expression analysis has been processed for differentially expressed genes, and it is necessary to figure the gene drive of each module. Furthermore, both GO function and KEGG pathway enrichment analysis were performed on the module genes. Comprehensively, the module gene set which was predicted according to hypergeometric testing was importantly regulated by both transcription factors (TFs) and non-coding RNAs (ncRNAs).

Conclusively, 16 co-expression modules were obtained. ACAT1 and ATBF1 would actively regulate in dysfunction modules, and thus they are identified as osteosarcoma-driven genes. Enrichment results showed that the module genes were significantly involved in transcription factor a non-metastatic osteosarcoma. It helps to identify core dysfunction modules and potential regulatory factors of the disease and improves understanding the underlying molecular association mechanisms between the two diseases.

To investigate the effects of miR-432 and miR-548c-3p on the proliferation and invasion of osteosarcoma cells.

A total of 67 cases of patients with osteosarcoma who came to the third Affiliated Hospital of Southern Medical University from April 2015 to May 2018 formed the experimental group, and 63 healthy individuals who came to this hospital for physical examination during the same period formed the control group. The expressions of miR-432 and miR-548c-3p in sera of each group were detected by RT-PCR to observe the changes of the expression levels of these miRs in the sera of the experimental and the control group, and the relationship between the expression levels of these miRs in the sera of patients with osteosarcoma and the grade of tumor differentiation and different pathological classification. GM-63 cells were selected as the target for in vitro experiments which were cultured and transfected. Before transfection, cells were divided into blank group (without transfection), negative control group (transfected with miR NC) and experimental group (transfected with miR-432 mimics/miR-548c-3p mimics).