Egholmwilhelmsen9518

Acute kidney injury (AKI) is a common complication of perinatal asphyxia and is associated with poorer short-term and long-term outcomes. This retrospective study describes the incidence of AKI in asphyxiated neonates who have received therapeutic hypothermia using the proposed modified Kidney Diseases Improving Global Outcomes (KDIGO) definition and investigates clinical markers that would allow earlier recognition of at-risk neonates. We included asphyxiated neonates who underwent therapeutic hypothermia between the period of January 2011 and May 2018 in our study. The serum creatinine levels within a week of birth were used in establishing AKI according to the modified KDIGO definition. Demographic data, resuscitation details, laboratory results and use of medications were collected and compared between the AKI and non-AKI groups to identify variables that differed significantly. A total of 66 neonates were included and 23 out of them (35%) were found to have AKI. The neonates with AKI had a lower gestational age (p = 0.006), lower hemoglobin level (p = 0.012), higher lactate level before and after therapeutic hypothermia (p = 0.013 and 0.03 respectively) and higher troponin-I level after therapeutic hypothermia (p  less then  0.001). After logistic regression analysis, elevated troponin-I after therapeutic hypothermia was independently associated with risk of AKI (OR 1.69, 95% CI 1.067-2.699, p = 0.025). The receiver operating curve showed that troponin-I after therapeutic hypothermia had an area under curve of 0.858 at the level 0.288 ng/ml. Our study concludes that the incidence of AKI among asphyxiated newborns who received therapeutic hypothermia is 35% and an elevated troponin-I level after therapeutic hypothermia is independently associated with an increased risk of AKI in asphyxiated newborns.The spontaneous eye blink rate (EBR) has been linked to different cognitive processes and neurobiological factors. It has also been proposed as a putative index for striatal dopaminergic function. While estradiol is well-known to increase dopamine levels through multiple mechanisms, no study up to date has investigated whether the EBR changes across the menstrual cycle. This question is imperative however, as women have sometimes been excluded from studies using the EBR due to potential effects of their hormonal profile. Fifty-four women were tested for spontaneous EBR at rest in three different phases of their menstrual cycle during menses (low progesterone and estradiol), in the pre-ovulatory phase (when estradiol levels peak and progesterone is still low), and during the luteal phase (high progesterone and estradiol). No significant differences were observed across the menstrual cycle and Bayes factors show strong support for the null hypothesis. Instead, we observed high intra-individual consistency of the EBR in our female sample. Accordingly, we strongly encourage including female participants in EBR studies, regardless of their cycle phase.Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by motor neuron loss that ultimately leads to fatal paralysis. Reducing levels or function of the tyrosine kinase, ephrin type-A receptor 4 (EphA4), has been suggested as a potential approach for slowing disease progression in ALS. Because EphA4 plays roles in embryonic nervous system development, study of constitutive knockout (KO) of EphA4 in mice is limited due to confounding phenotypes with homozygous knockout. We used a tamoxifen-inducible EphA4 conditional KO mouse to achieve strong reduction of EphA4 levels in postnatal mice to test for protective effects in the SOD1G93A model of ALS. We found that EphA4 KO in young mice, but not older adult mice, causes defects in muscle function, consistent with a prolonged postnatal role for EphA4 in adolescent muscle growth. When testing the effects of inducible EphA4 KO at different timepoints in SOD1G93A mice, we found no benefits on motor function or disease pathology, including muscle denervation and motor neuron loss. Our results demonstrate deleterious effects of reducing EphA4 levels in juvenile mice and do not provide support for the hypothesis that widespread EphA4 reduction is beneficial in the SOD1G93A mouse model of ALS.Pre-eclampsia is a severe hypertensive disorder of pregnancy and could lead to severe maternal morbidities and death. Our study aimed to develop and validate a prognostic prediction model for severe maternal outcomes among Chinese population with pre-eclampsia. We conducted a 10-year cohort study in a referral center by collecting all pregnant women who diagnosed as pre-eclampsia and delivered from 2005 to 2014. A composite of severe maternal outcomes, including maternal near-miss defined by World Health Organization, cortical blindness/retinal detachment, temporary facial paralysis and maternal death, were adopted. PLX3397 molecular weight We used logistic regression model to develop Model 1 by retaining the predictors of p  less then  0.05, and further conducted Model 2 by adding quadratic terms and interaction terms to Model 1. We undertook a bootstrapping validation and estimated the model performance. A total of 397 pregnant women suffered from severe maternal outcomes among 2,793 eligible participants, with an incidence of 14.21% (95% confidence interval (CI) 12.91%-15.51%). Of 13 predictors were finally selected in Model 1. Combined with quadratic and interactive terms, the Model 2 showed higher area under the ROC curve (82.2%, 95% CI 79.6%-84.7%) and good calibration. By the bootstrapping validation, similar model performances were present.Quartz can increase oxidative stress, lipid peroxidation, and inflammation. The objective of this study was to explore the volatile biomarkers of quartz-induced lung injury using a lung alveolar cell model. We exposed the human alveolar A549 cell line to 0, 200, and 500 μg/mL quartz particles for 24 h and used gas chromatography-mass spectrometry to measure the volatile metabolites in the headspace air of cells. We identified ten volatile metabolites that had concentration-response relationships with particles exposure, including 1,2,4-oxadiazole, 5-(4-nitrophenyl)-3-phenyl- (CAS 28825-12-9), 2,6-dimethyl-6-trifluoroacetoxyoctane (CAS 61986-67-2), 3-buten-1-amine, N,N-dimethyl- (CAS 55831-89-5), 2-propanol, 2-methyl- (CAS 75-65-0), glycolaldehyde dimethyl acetal (CAS 30934-97-5), propanoic acid, 2-oxo-, ethyl ester (CAS 617-35-6), octane (CAS 111-65-9), octane, 3,3-dimethyl- (CAS 4110-44-5), heptane, 2,3-dimethyl- (CAS 3074-71-3) and ethanedioic acid, bis(trimethylsilyl) ester (CAS 18294-04-7). The volatile biomarkers are generated through the pathways of propanoate and nitrogen metabolism.