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Furthermore, patients with high FOXP4-AS1 expression level had poorer OS and PFS than those with low FOXP4-AS1 expression. Finally, univariate and multivariate Cox proportional hazards regression analysis found that the T stage, lymph node metastasis, clinical stage, FOXP4-AS1 expression might be independent risk factors for OS and PFS of nasopharyngeal carcinoma patients. This study firstly clarified that FOXP4-AS1 was overexpressed in nasopharyngeal carcinoma. And FOXP4-AS1 may act as a diagnostic and prognostic biomarker, and hopeful therapeutic target for nasopharyngeal carcinoma patients.Tuber melanosporum Vittad. (Black or Périgord truffle) is a truffle native to the Mediterranean Southern Europe, popular for its unique flavor, and has great economic importance. The present work focused on assessing the possibility of cultivating T. melanosporum associated with Quercus robur L. in the desert climate of Saudi Arabia. The plantation was initiated in November 2018 by planting 271 oak seedlings in the Al-Qassim desert area and checked for survival and ectomycorrhiza development after 1.5 years of plantation maintenance. Amongst the 271 seedlings planted, 243 plants survived two harsh seasons (2019 and 2020), and the randomly selected and tested seedlings were still mycorrhized with T. melanosporum. The mycorrhization level with T. melanosporum was between 5 and 35% of all fine roots, and the share of contaminant ectomycorrhiza was low. In comparison to other areas where T. melanosporum is successfully cultivated, the Al-Qassim desert area has 10-15 °C higher average summer temperatures and a low total annual precipitation, which necessitates regular irrigation of the plantation. This work opens the avenue for an adapted, yet sustainable cultivation of T. melanosporum-inoculated oak tree in a desert climatic condition and introduces new opportunities of the agro-forest business in Saudi Arabia and GCC region.The present study characterized the potential probiotic properties of Pediococcus acidilactici TMAB26 strain isolated from traditional Indian tomato pickle, and evaluated its possible therapeutic applications as an anti-cancer and anti-inflammatory agent in vitro. The 16S rRNA sequencing and primary screening demarcated TMAB26 strain as an ideal probiotic candidate, with distinctive properties of acid tolerance (58.02% at pH 2.5), bile tolerance (55.53% at 0.5%), and efficient adherence to the mucosal surface of the human intestinal cells in vitro, along with antagonistic, anti-inflammatory, and anti-cancer properties. The strain exhibited antagonism against standard intestinal pathogenic strains Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, E. coli, Klebsiella pneumonia, and Salmonella typhi with zones of inhibition in the range of 6-18 mm. The cytotoxicity evaluation of the probiotic isolate TMAB26 culture supernatant (11 dilution) showed significant cytotoxicity on HT-29 (94.91% ± 1.27) and Caco-2 (92.63% ± 0.63) cancer cells when compared to that of the peripheral blood mononuclear cells (PBMCs) alone. Furthermore, the strain culture supernatant reduced the mRNA levels of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) by threefold, Interleukin-6 (IL-6) by eightfold and increased the mRNA levels of the anti-inflammatory cytokine Interleukin-10 (IL-10) in lipopolysaccharide (LPS) pretreated HT-29 and peripheral blood mononuclear cells (PBMCs), suggesting the potential role of TMAB26 isolate, i.e., Pediococcus acidilactici MTCC 13014 in alleviating gut inflammation.

The online version contains supplementary material available at 10.1007/s13205-020-02570-1.

The online version contains supplementary material available at 10.1007/s13205-020-02570-1.This study aimed to explore the effect of Dendrobium officinale (DO) on the diversity of intestinal mucosal flora in high-fat diet mice and provided an experimental basis for the development and research of DO and its series products. Twenty-four mice were randomly assigned to four equal groups of six mice, namely the control (bcm) group, model (bmm) group, Dendrobium officinale (bdm) group, and positive control (bjm) group. Mice in the bdm group were administrated at the dose of 2.37 g·kg-1·days-1, and those in bjm group were given the Lipid-lowering decoction at the concentration of 1.19 g·kg-1·days-1, and sterile water was used as a placebo control twice a day for 40 consecutive days. We measured the dynamic weight changes and intestinal mucosal flora changes in mice. The analysis showed that DO had a regulatory effect on weight change induced by a high-fat diet in mice. VLS-1488 solubility dmso DO could also regulate the changes in the diversity of the intestinal mucosa of mice, which was specifically reflected in the changes of Chao 1, ACE, Shannon and Simpson index. The sample information of the bdm group was relatively concentrated, but the distance from the bmm group was relatively scattered. The relative abundance results showed dominant bacteria phylum (such as Bacteroidetes, Actinobacteria, Verrucomicrobia) and bacterial genus (such as Bifidobacterium, Ruminococcus, Ochrobactrum) in the intestinal mucosa of the four groups. And significant differences in the major microbiota between the bdm and bjm groups. In addition, DO changed the carbohydrate, energy, and amino acid metabolism of intestinal mucosal flora. To sum up, DO has a regulatory effect on weight change induced by high-fat diet in mice and can improve the diversity of intestinal mucosal flora, promote the abundance of Ochrobactrum, inhibit the abundance of Bifidobacterium and Ruminococcus, and influence the intestinal flora to positively affect high-fat diet-induced negative effects in mice.Biphasic acid hydrolysates and enzymatic hydrolysates from carbohydrate-rich Prosopis juliflora, an invasive perennial deciduous shrub of semi-arid regions, were used for bioethanol production. Saccharomyces cerevisiae and Pichia stipitis were used for fermentation of hexoses and pentoses. P. juliflora acid hydrolysate with an initial sugar concentration of 18.70 ± 0.16 g/L was concentrated to 33.59 ± 0.52 g/L by vacuum distillation. The concentrated hydrolysate was pretreated and fermented by mono- and co-culture methods either singly or in combination with enzyme hydrolysate and ethanol yields were compared. Monoculture with S. cerevisiae (VS3) and S. cerevisiae (NCIM3455) yielded maximum ethanol of 36.6 ± 1.83 g/L and 37.1 ± 1.86 g/L with a fermentation efficiency of 83.94 ± 4.20% and 84.20 ± 4.21%, respectively, after 36 h of fermentation. The ethanol yield obtained was 0.428 ± 0.02 g/g substrate and 0.429 ± 0.02 g/g substrate with a productivity of 1.017 ± 0.051 g/L/hand 1.031 ± 0.052 g/L/h, respectively.