Kangshannon7061

The cyanobacterial circadian clock in Synechococcus elongatus consists of three proteins, KaiA, KaiB, and KaiC. KaiA and KaiB rhythmically interact with KaiC to generate stable oscillations of KaiC phosphorylation with a period of 24 h. The observation of stable circadian oscillations when the three clock proteins are reconstituted and combined in vitro makes it an ideal system for understanding its underlying molecular mechanisms and circadian clocks in general. These oscillations were historically monitored in vitro by gel electrophoresis of reaction mixtures based on the differing electrophoretic mobilities between various phosphostates of KaiC. As the KaiC phospho-distribution represents only one facet of the oscillations, orthogonal tools are necessary to explore other interactions to generate a full description of the system. However, previous biochemical assays are discontinuous or qualitative. To circumvent these limitations, we developed a spin-labeled KaiB mutant that can differentiate KaiC-bound KaiB from free KaiB using continuous-wave electron paramagnetic resonance spectroscopy that is minimally sensitive to KaiA. Similar to wild-type (WT-KaiB), this labeled mutant, in combination with KaiA, sustains robust circadian rhythms of KaiC phosphorylation. This labeled mutant is hence a functional surrogate of WT-KaiB and thus participates in and reports on autonomous macroscopic circadian rhythms generated by mixtures that include KaiA, KaiC, and ATP. Quantitative kinetics could be extracted with improved precision and time resolution. Teniposide We describe design principles, data analysis, and limitations of this quantitative binding assay and discuss future research necessary to overcome these challenges.We investigate the charge separation dynamics provided by carrier surface trapping in CdSe/CdS core/shell nanoplatelets by means of a three-laser-beam pump-orientation-probe technique, detecting the electron spin coherence at room temperature. Signals with two Larmor precession frequencies are found, which strongly differ in their dynamical characteristics and dependencies on pump power and shell thickness. The electron trapping process occurs on a time scale of about 10 ns, and the charge separation induced thereby has a long lifetime of up to hundreds of microseconds. On the other hand, the hole trapping requires times from subpicoseconds to hundreds of picoseconds, and the induced charge separation has a lifetime of a few nanoseconds. With increasing CdS shell thickness the hole trapping vanishes, while the electron trapping is still detectable. These findings have important implications for understanding the photophysical processes of nanoplatelets and other colloidal nanostructures.The continuous production of macroscale filaments of 17 μm in diameter comprising aligned TEMPO-oxidized cellulose nanofibrils (CNFs) is conducted using a field-assisted flow-focusing process. The effect of an AC external field on the material's structure becomes significant at a certain voltage, beyond which augmentations of the CNF orientation factor up to 16% are obtained. Results indicate that the electric field significantly contributes to improve the CNF ordering in the bulk, while the CNF alignment on the filament surface is only slightly affected by the applied voltage. X-ray diffraction shows that CNFs are densely packed anisotropically in the plane parallel to the filament axis without any preferential out of plane orientation. The improved nanoscale ordering combined with the tight CNF packing yields impressive enhancements in mechanical properties, with stiffness up to 25 GPa and more than 63% (up to 260 MPa), 46% (up to 2.8%), and 120% (up to 4.7 kJ/m3) increase in tensile strength, strain-to-failure, and toughness, respectively. This study demonstrates for the first time the control over the structural ordering of anisotropic nanoparticles in a dynamic system using an electric field, which can have important implications for the development of sustainable alternatives to synthetic textiles.We report a self-template and facile pyrolysis method to synthesize Fe/Fe3C-decorated metal-nitrogen-carbon mesoporous nanospheres, of which preserved plum-like and hollow structures can be simply engineered via controlling the thickness of the outermost polydopamine layer in the precursors. The preserved plum-like structure is demonstrated to show a large electrochemically active surface area and facilitate fast charge transfer, in comparison with the hollow one. The catalytic activities of metal-nitrogen-carbon and nitrogen-doped carbon active sites in the outer carbon layer toward oxygen reduction are improved under the activation of the encased Fe species. Hence, preserved plum-like structures exhibit excellent catalytic kinetics toward the oxygen reduction reaction in alkaline media. The mass activity of 21.0 mA mgcatalyst-1 at 0.9 V vs RHE is achieved and the half-wave potential is 50 mV more positive than that of the Pt/C catalyst with the same mass loading. Moreover, the outer carbon layer endows the tolerance of strong acidic and alkaline environments, resulting in good durability. Our study proposes a simple strategy for the rational design of novel transition metal carbide-based catalysts, making it a promising candidate for replacing platinum-group metal catalysts in low-temperature fuel cells.Although flexible, transparent, and conductive materials are increasingly required for electromagnetic interference (EMI) shielding applications in foldable and wearable electronics, it remains a great challenge to achieve outstanding shielding performances while retaining high light transmittances. Herein, a multiscale structure optimization strategy is proposed to fabricate a transparent and conductive silver nanowire (AgNW) film with both high EMI shielding performance and high light transmittance by a scalable spray-coating technique. By decorating with a Ti3C2Tx MXene coating, the connection and integrity of the AgNW network are greatly improved by welding the nanowire junctions. Compared to a neat AgNW film (21 dB) with the same AgNW density, the Ti3C2Tx MXene-welded AgNW film shows a much higher EMI shielding performance (34 dB) with better mechanical and environmental stabilities. Furthermore, the layered structure design on the macroscopic scale results in an even higher EMI shielding efficiency of 49.