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In addition, the minimal inhibition concentrations (MICs) of QLS/ZnO NCs towards E. coli and S. aureus were both 100 μg/mL, and the minimum bactericidal concentrations (MBCs) were 100 μg/mL and 200 μg/mL, respectively. Moreover, with the incorporation of QLS/ZnO NCs into polyurethane films, the composite films showed excellent antibacterial activity, strong tensile strength and enhanced ultraviolet light blocking performance.Respiratory syncytial virus (RSV) infection is the most common clinical infectious disease threatening the safety of human life. Herein, we provided a sensitive and specific method for detection and differentiation of RSV subgroups A (RSVA) and B (RSVB) with colorimetric toehold switch sensors in a paper-based cell-free system. In this method, we applied the toehold switch, an RNA-based riboswitch, to regulate the translation level of β-galactosidase (lacZ) gene. In the presence of target trigger RNA, the toehold switch sensor was activated and the expressed LacZ hydrolyzed chromogenic substrates to produce a colorimetric result that can be observed directly with the naked eye in a cell-free system. In addition, nucleic acid sequence-based amplification (NASBA) was used to improve the sensitivity by amplifying target trigger RNAs. Under optimal conditions, our method produced a visible result for the detection of RSVA and RSVB with the detection limit of 52 aM and 91 aM, respectively. The cross-reaction of this method was validated with other closely related respiratory viruses, including human coronavirus HKU1 (HCoV-HKU1), and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Furthermore, we used the paper-based carrier material that allows stable storage of our detection elements and rapid detection outside laboratory. In conclusion, this method can sensitively and specifically differentiate RSVA and RSVB and generate a visible colorimetric result without specialized operators and sophisticated equipment. Based on these advantages above, this method serves as a simple and portable detector in resource-poor areas and point-of-care testing (POCT) scenarios.Chemogenetic property of mercuric ion (Hg2+) was investigated as a specific hypercalcemia actuator in the neuronal spinal cord cell manipulation by Zeta-based potentiometric bio-sensing analysis via introducing a novel array-based Hg2+ bio-sensor. For this purpose, the array of a two-electrode system including Ag/AgCl (sat'd Cl-) as reference electrode and a paste nano-composite as the indicator electrode was utilized. The indicator electrode was made of activated multi-walled carbon nanotubes as conductive support, a grounded slice of sheep's spinal cord as natural neuron stem cells (ionophore), and oxalate ion as both the dispersed phase and cationic site. Under optimum conditions by one-at-a-time method, a two-linear range between 1.3 × 10-4- 6.5 × 10-12 and 2.7 × 10-14- 1.4 × 10-21 mol L-1 with correlation coefficients (R2) of 0.96 and 0.99, respectively, and response time (t90) of maximum 5.0 min were approximated. The percentages of relative standard deviation were estimated to be 4.05 (repeatability, n = 10) and 6.14 (reproducibility, n = 12). The detection limit was estimated to be sub 5.3 × 10-22 mol L-1 based on the X̄b+3Sb. The reliability of this phenomenon was evidenced by different analytical techniques. The Zeta-based electrical response was therefore attributed to highly Ca2+ pumping from the stem cells ionic channel gates as the proposed mechanistic behavior of the spinal cord. Actuating (triggering) the stem cells by Hg2+ consequently led to generate significant Zeta potential as the proposed mechanism. The results pointed to the potentiometric responsibility of a protein with gram molecular weight of 66.2 ± 0.3 KCU in the stem cell matrix as a specific hypercalcemia actuator.We present herein the very first amperometric biosensor for the quantitative determination of glycine in diverse biological fluids. The biosensor is based on a novel quinoprotein that catalyzes the oxidation of glycine with high specificity. This process is coupled to the redox conversion of Prussian blue in the presence of hydrogen peroxide originating from the enzymatic reaction. The optimized tailoring of the biosensor design consists of the effective encapsulation of the quinoprotein in a chitosan matrix with the posterior addition of an outer Nafion layer, which is here demonstrated to suppress matrix interference. This is particularly important in the case of ascorbic acid, which is known to influence the redox behavior of the Prussian blue. The analytical performance of the biosensor demonstrates fast response time ( less then 7 s), acceptable reversibility, reproducibility, and stability ( less then 6% variation) as well as a wide linear range of response (25-500 μM) that covers healthy (and even most unhealthy) physiological levels of glycine in blood/serum, urine and sweat. A total of 6 real samples from healthy patients and animals were analyzed two serum, two urine and two sweat samples. learn more The results were validated via commercially available fluorescence kit, displaying discrepancy of less than 9% in all the samples. The unique analytical features and effortless preparation of the new glycine biosensor position it at the forefront of current technologies towards decentralized clinical applications and sport performance monitoring.Ochratoxin A (OTA), a toxic secondary metabolite produced via various fungus, poses a serious threat to the health of human beings and animals. In this paper, an aptasensor for OTA detection based on gold nanoparticles decorated molybdenum oxide (AuNPs-MoOx) nanocomposites, hybridization chain reaction (HCR) and a restriction endonuclease (Nb.BbvCI)-aided walker DNA machine was successfully constructed. In this electrochemical platform, the HCR was also used to embed more electrical signal molecules of methylene blue (MB) on silver nanoparticles (AgNPs) to achieve signal amplification. Under the optimum conditions, after adding OTA and Nb.BbvCI in turn and responding adequately under appropriate conditions, aptamer-DNA (6-DNA) carries the OTA away from the electrode surface, and walker DNA was hybridized autonomously with 5-DNA, releasing a large amount of 5'-DNA with the help of Nb.BBVCI. Finally, the electrochemical signal obtained by differential pulse voltammetry (DPV) was weakened. As an artificial and popular signal amplification technique, the DNA walking machine greatly improved the sensitivity.