Matthiesenhoff5918

Galectin-1 (Gal-1) and galectin-3 (Gal-3) are multifunctional glycan-binding proteins, expressed in the brain and in its limbic structures that are involved in behavioral control. Gal-1 induces the expression of the brain-derived neurotrophic factor (BDNF) and promotes adult neural stem cells proliferation, biological events impaired in stress-related psychiatric disorders, such as depression and anxiety. Despite that, there is no evidence regarding galectin involvement in emotional control during stressful situations. Thus, we analyzed the behavioral phenotype of Gal-1 or Gal-3 knock-out mice (Gal-1 KO or Gal-3 KO) in different experimental models predictive of depressive and compulsive-like behaviors. METHODS C57BL-6 Gal-1 KO, Gal-3 KO, and wild-type mice (WT) were analyzed under the open field test (OFT) and, 6 h later, under the forced swim test (FST). Additionally, independent groups of male mice, lacking galectins or not, were exposed to the tail suspension test (TST) or to the marble burying test (MBT). The hippocampus and prefrontal cortex (PFC) of the mice submitted to MBT were dissected to access BDNF levels. RESULTS Both Gal-1 and Gal-3 KO mice showed increased time of immobility in the FST and in the TST compared to WT animals, thus reflecting an impaired stress-coping behavior. Additionally, Gal-1 and Gal-3 KO female mice presented increased compulsive-like behavior in the MBT, without significant changes in the locomotor activity. BDNF levels were found to be decreased in the PFC of Gal-1 KO mice. DISCUSSION Our results demonstrate that the absence of either endogenous Gal-1 and Gal-3 impairs stress-coping and increases compulsive-like behavior, suggesting that Gal-1 and Gal-3 are involved in the neurobiology of depression and obsessive-compulsive-like disorder. The current industrialized food system that is the predominant model of production in the United States and increasingly in other regions of the world has been recognized as a major contributor to greenhouse gas emissions, public health crises, environmental and soil health degradation, animal welfare abuses, labor crises and more. This article describes the evolution and design of food systems over time and the current assessment that a new system is required that focuses on planetary health and the health and well-being of humans and animals. The article describes the growth in entrepreneurship in the food system today and concludes that innovation is in need of increased support in order to advance a new operating system for how we grow, produce and distribute food. While both men and women gain weight as a side effect of antipsychotic treatment, studies in mice have found only female mice are susceptible to weight gain. Therefore, to we set out to identify a strain of male mice that gain significant weight in response to APs which could better model AP-induced weight gain observed in humans. These studies determined that male Balb/c mice developed late onset olanzapine-induced weight gain. Patients often take APs for many years and thus understanding AP-mediated changes in energy expenditure and body weight regulation is particularly important. Prader-Willi syndrome (PWS) is a rare genetic form of hyperphagia leading to severe obesity, accompanied by endocrine, musculoskeletal, and neurological dysfunction. PWS is caused by the inactivation of contiguous genes on chromosome 15q11-q13, and mice with gene-targeted mutations in one or more of these PWS genes recapitulate PWS-like phenotypes. In addition to evaluating the potential effectiveness of a therapeutic for the treatment of PWS, animal models can be used to elucidate the deficiencies in appetitive and energy balance pathways that lead to hyperphagia and obesity. Various therapeutics have been tested for their effects on ingestive behavior, hyperphagia, and obesity in clinical trials for PWS, with encouraging preliminary results on small groups of participants with PWS. Here, we summarize ingestive behavior-related therapeutics tested in PWS animal models and summarize published data from clinical trials that have evaluated the effect of therapeutics on ingestive behavior in individuals with PWS. We then discuss strategies to accelerate the discovery and translation of therapies into clinical practice in PWS. One of the compounds generally found in the residues of the coffee and tea industries is caffeine, which in high concentration is toxic to various organisms, making it necessary to find an adequate treatment for these residues. Biotechnological treatments using enzymes can be an alternative to valorize and detoxify these residues. However, mixtures of substrates have not been evaluated to improve production. Therefore, the present investigation aimed to study the effect of different proportions of sorghum-coffee pulp mixtures as a substrate in solid-state fermentation with the fungus Rhizopus oryzae (MUCL 28168) for the production of n-demethylases. To evaluate the synergistic and antagonistic effects of coffee pulp and sorghum mixtures on n-demethylase enzyme production, a simplex-centroid design, using four levels 1 (100%), 1/4 (25%), 1/2 (50%), 3/4 (75%). ML198 nmr Results obtained were favorable, achieving a caffeine demethylase activity of 18.762 U/g, and reducing the caffeine content in the coffee pulp. Exposure to heavy metals is a major threat to aquatic bodies and is a global concern to our four main spheres of the earth viz. atmosphere, biosphere, hydrosphere, and lithosphere. The biosorption of pollutants using naturally inspired sources like microalgae has considerable advantages. Diatoms are the most dominant and diverse group of phytoplankton which accounts for 45% oceanic primary productivity. They perform a pioneer part in the biogeochemistry of metals in both fresh and marine water ecosystems. The diatoms play a significant role in degradation, speciation, and detoxification of chemical wastes and hazardous metals from polluted sites. Herein, an overview is presented about the ability of diatom algae to phycoremediate heavy metals by passive adsorption and active assimilation from their aqueous environments with an emphasis on extracellular and intracellular mechanisms involved in contaminant uptake through the frustules for preventing heavy metal toxicity.