Funderhvidberg4106

daptively important) were often identified as markers supporting species branches. (4) In all five Littorina species British populations were separated from the European mainland populations, possibly reflecting their recent phylogeographic history. Altogether our study shows that proteomic data, when interpreted in the context of DNA-based phylogeny, can bring additional information on the evolutionary history of species. © 2020 Maltseva et al.Background Indochelys spatulata is an extinct turtle from the Early to Middle Jurassic Kota Formation of the Pranhita-Godavari Gondwana basin, India. The holotype and previously only known specimen is a partially eroded shell that had been collected near Kota village, north of Sironcha, in Maharashtra State. Phylogenetic analyses have consistently suggested placement at the base of the clade Mesochelydia. Methods We here figure and describe the holotype of Indochelys spatulata and two new specimens, which were collected from the Kota Formation near Kistapur village, Telengana State, about 60 km NW from the type locality. We furthermore explore the relationships of this fossil turtle by updating its scoring based on all available material in the most recent analysis of basal turtle relationships. Results The revision of the holotype of Indochelys spatulata provides minor adjustments to the morphology of this specimen, in particular recognition of a transverse break across the carapace, presence of only eight neurals, of which the eight is octagonal, and presence of a pathological element located between neurals VII and VIII. The new material provides new anatomical insights, in particular presence of a broad cervical, a vertebral V that inserts deeply into vertebral IV, narrow pleurals within increasingly short posteromedial contacts with the vertebrals towards the posterior, at least three pairs of musk duct foramina, and numerous insights into the morphology of the girdles and stylopodium. In combination, all material allows affirming the validity of Indochelys spatulata with confidence. The phylogenetic analysis affirms the placement of Indochelys spatulata as a basal mesochelydian, but cannot resolve its relationships relative to the roughly coeval Condorchelys antiqua and Kayentachelys aprix. © 2020 Joyce and Bandyopadhyay.Pitayas (Hylocereus spp.) is an attractive, highly nutritious and commercially valuable tropical fruit. However, low-temperature damage limits crop production. Genome of pitaya has not been sequenced yet. In this study, we sequenced the transcriptome of pitaya as the reference and further investigated the proteome under low temperature. By RNAseq technique, approximately 25.3 million reads were obtained, and further trimmed and assembled into 81,252 unigene sequences. The unigenes were searched against UniProt, NR and COGs at NCBI, Pfam, InterPro and Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and 57,905 unigenes were retrieved annotations. Among them, 44,337 coding sequences were predicted by Trandecoder (v2.0.1), which served as the reference database for label-free proteomic analysis study of pitaya. Here, we identified 116 Differentially Abundant Proteins (DAPs) associated with the cold stress in pitaya, of which 18 proteins were up-regulated and 98 proteins were down-regulated. KEGG analysis and other results showed that these DAPs mainly related to chloroplasts and mitochondria metabolism. In summary, chloroplasts and mitochondria metabolism-related proteins may play an important role in response to cold stress in pitayas. © 2020 Zhou et al.Histone deacetylases (HDACs) are key epigenetic factors in regulating chromatin structure and gene expression in multiple aspects of plant growth, development, and response to abiotic or biotic stresses. Many studies on systematic analysis and molecular function of HDACs in Arabidopsis and rice have been conducted. However, systematic analysis of HDAC gene family and gene expression in response to abiotic and biotic stresses has not yet been reported. In this study, a systematic analysis of the HDAC gene family in maize was performed and 18 ZmHDACs distributed on nine chromosomes were identified. Phylogenetic analysis of ZmHDACs showed that this gene family could be divided into RPD3/HDA1, SIR2, and HD2 groups. Tissue-specific expression results revealed that ZmHDACs exhibited diverse expression patterns in different tissues, indicating that these genes might have diversified functions in growth and development. Expression pattern of ZmHDACs in hormone treatment and inoculation experiment suggested that several ZmHDACs might be involved in jasmonic acid or salicylic acid signaling pathway and defense response. Interestingly, HDAC genes were downregulated under heat stress, and immunoblotting results demonstrated that histones H3K9ac and H4K5ac levels were increased under heat stress. These results provide insights into ZmHDACs, which could help to reveal their functions in controlling maize development and responses to abiotic or biotic stresses. ©2020 Zhang et al.Background High-density conditions are global issues that threaten the sustainable management of plantations throughout the world. Monocultures and untimely management practices have identically resulted in the simplex of community structures, decreases in biodiversity, and long-term productivity losses in plantations China. read more The most popular measure which is commonly used to address these issues is thinning, which potentially results in increases in the development of understory plants in plantations. However, there is limited information currently available regarding the community composition of understory vegetation and the associated environmental factors, which has limited the sustainable management of China's fir plantation ecosystems. Method In the present study, a thinning experiment was implemented which included a control check (CK no thinning), as well as low intensity thinning (LIT 20%), moderate intensity thinning (MIT 33%), and high intensity thinning (HIT 50%) in Chinese fir plantations located ven by the tree densities, slope positions, and soil nutrient contents. ©2020 Xu et al.