Abdibland1262

ental resistance.We investigated the in vitro effects of pyriproxyfen on ionic balance in the testis of the zebrafish by measuring 45Ca2+ influx. In vivo pyriproxyfen treatment was carried out to study oxidative stress, and conduct morphological analysis of the testis and liver. Whole testes were incubated in vitro with/without pyriproxyfen (10-12, 10-9 or 10-6 M; 30 min) and 45Ca2+ influx determined. To study pyriproxyfen's mechanism of action, inhibitors/activators of ionic channels or pumps/exchangers, protein kinase inhibitors or a calcium chelator were added 15 min before the addition of 45Ca2+ and pyriproxyfen. We evaluated the in vivo effects of 7 day exposure to waterborne pyriproxyfen (10-9 M) on reactive oxygen species (ROS) formation, lipid peroxidation, and reduced glutathione content (GSH), glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) and γ-glutamyltransferase (GGT) activity. Morphological analyses of the testis and liver were carried out after in vivo exposure of D. rerio to pyriprdings indicate that these alterations may contribute to pyriproxyfen toxicity and spermatogenesis disruption.Nuclear factor-erythroid 2-related factor-2 (Nrf2) is an important modulator of cellular responses against Cd in mammalian cells. However, whether such modulation is conserved in Marsupenaeus japonicas remains unknown.In our study, the shrimps were injected with dsRNA targeting Nrf2 at 4 μg g-1 body weight (b.w.) or sulforaphane (SFN) at 5 μg g-1 b.w., and then were exposed to 40 mg L-1 CdCl2 for 48 h. After Nrf2 knockdown, the Cd content increased, but decreased in the SFN group. This suggested that Nrf2 could promote Cd excretion. A terminal deoxynulceotidyl transferase nick-end-labeling (TUNEL) assay revealed that the Nrf2 knockdown increased the number of apoptotic cells in M. japonicas, while SFN decreased the number of apoptotic cells. After Nrf2 knockdown, the total antioxidant capacity (T-AOC), superoxide dismutase (Sod) activity, and related gene expression decreased significantly, while the malondialdehyde (MDA) content increased remarkably. By contrast, SFN injection alleviated the oxidative stress, as evidenced by increased T-AOC, Sod activity, sod mRNA expression and a reduced MDA content. Similarly, detoxification related enzyme activities (ethoxyresorufin O-deethylase and glutathione-S-transferase (GST)) and their corresponding gene expressions (cyp3a (cytochrome P450 family 3 subfamily A) and gst) were suppressed in the ds-Nrf2 injection group, while they were elevated in the SFN group. In addition, ds-Nrf2 activated mitochondrial apoptotic pathway, as evidenced the mRNA and protein levels of caspase-3, Bcl2 associated X protein (Bax), and p53, while SFN treatment suppressed them. These results displayed that in M. japonicus Cd-induced cellular oxidative damage probably acts via the Nrf2 pathway.Post-harvest crop residue burning is extensively practiced in North India, which results in enhanced particulate matter (PM) concentrations. This study explores the PM2.5 (particulate matter with aerodynamic diameter ≤ 2.5 μm) emissions during various time periods (pre-monsoon, monsoon, and post-monsoon) over the biomass burning source region in Beas, Punjab. The PM2.5 concentrations during the pre-monsoon period (106-458 μg m-3) and the post-monsoon period (184-342 μg m-3) were similar but much higher than concentrations during the monsoon season (23-95 μg m-3) due to enhanced wet deposition. However, the carbonaceous aerosol fraction in PM2.5 was nearly double in the post-monsoon season (∼27%) than the pre-monsoon period (∼15%). A higher contribution of secondary organic carbon (SOC) observed during the pre-monsoon season can be attributed to enhanced photochemical activity in dry conditions. Stable carbon isotope ratio (δ13C value) of ambient PM allowed elucidation of contributing sources. δ13CTC correlation with SOC during post-monsoon and pre-monsoon periods suggests significant influence of secondary formation processes during both time periods. The concentrations of carbon fractions in sampled sources and aerosols suggests contribution of biofuels, resulting in enhanced PM concentration at this location. δ13CTC values of pre- and post-monsoon samples show dominance of freshly emitted aerosols from local sources. Impact of biomass and biofuel combustion was also confirmed by biomass burning K+BB tracer, indicating that major agriculture residue burning occurred primarily during nighttime. click here C3 plant derived aerosols dominated at the sampling location during the entire sampling duration and contributed significantly during the pre-monsoon season. Whereas, both fossil fuel and C3 plant combustion contributed to the total mass of carbonaceous aerosols during the post-monsoon and monsoon seasons.Marine canopies formed by seagrass and other coastal vegetated ecosystems could act as sinks of microplastics for being efficient particle traps. Here we investigated for the first time the occurrence of microplastic retention by marine canopies in a hydraulic flume under unidirectional flow velocities from 2 to 30 cm s-1. We used as model canopy-forming species the seagrass Zostera marina with four canopy shoot density (0, 50, 100, 200 shoots m-2), and we used as microplastic particles industrial pristine pellets with specific densities from 0.90 to 1.34 g cm-3 (polypropylene PP; polystyrene PS; polyamide 6 PA; and polyethylene terephthalate PET). Overall, microplastics particles transported with the flow were retained in the seagrass canopies but not in bare sand. While seagrass canopies retained floating microplastics (PP) only at low velocities ( less then 12 cm s-1) due to a barrier created by the canopy touching the water surface, the retention of sinking particles (PS, PA, PET) occurred across a wider range of flow velocities. Our simulations revealed that less dense sinking particles (PS) might escape from the canopy at high velocities, while denser sinking particles can be trapped in scouring areas created by erosive processes around the eelgrass shoots. Our results show that marine canopies might act as potential barriers or sinks for microplastics at certain bio-physical conditions, with the probability of retention generally increasing with the seagrass shoot density and polymer specific density and decreasing with the flow velocity. We conclude that seagrass meadows, and other aquatic canopy-forming ecosystems, should be prioritized habitats in assessment of microplastic exposure and impact on coastal areas since they may accumulate high concentration of microplastic particles that could affect associated fauna.