Lemmingmckenzie1115

Our results demonstrated that the fabricated collagen sponges could be used as perfect hemostatic dressings. Early diagnosis is the key to the good prognosis of breast cancer. At present, clinical tumor diagnosis is mainly through serum analysis, which is painful and can only detect relatively advanced tumors that have already metastasized from the glands into the blood circulation. Here, we developed an earlier diagnostic method (before tumor cells entering the blood) of breast cancers through a convenient and painless process with blood-free samples. The microneedles were utilized to insert into the animals' testing areas, while the tissue fluid was collected through our synthesized breathable thin film. The obtained tissue fluid sample was then incubated to form blue products. In the area where tumors occurred, the blue changes were more obvious than the healthy area, a semi-localization and semi-quantitative detection of the tumorous area thus could be realized. The results of corresponding animal experiments showed that, after the injection of tumor cells, the proposed nano-Ag based colorimetric method can detect the occurrence of breast cancers in 7 days. What is more, these early tumors could be effectively suppressed through classical DOX treatment. For comparison, the classical blood test needed 14 days to validate the occurrence of breast cancers. The subsequent human tests further demonstrated the feasibility of the present method. The development of this work could provide a more convenient, accurate and comfortable technology to support for the early screening and diagnosis of cancer patients, so as to fundamentally reduce the mortality of the breast cancers. We report a straightforward route for the preparation of flexible, electrochemically stable and easily functionalizable poly(3,4-ethylenedioxythiophene) (PEDOT) composite films deposited on PET foils as biosensing platforms. For this purpose, poly(allylamine) hydrochloride (PAH) was blended with PEDOT to provide amine-bearing sites for further biofunctionalization as well as to improve the mechanical properties of the films. The conducting PEDOT-PAH composite films were characterized by cyclic voltammetry, UV-vis and Raman spectroscopies. An exhaustive stability study was carried out from the mechanical, morphological and electrochemical viewpoint. Subsequent sugar functionalization of the available amine groups from PAH allowed for the specific recognition of lectins and the subsequent self-assembly of glycoenzymes (glucose oxidase and horseradish peroxidase) concomitant with the prevention of non-specific protein fouling. The platforms presented good bioelectrochemical performance (glucose oxidation and hydrogen peroxide reduction) in the presence of redox mediators. The developed composite films constitute a promising option for the construction of all-polymer biosensing platforms with great potential owing to their flexibility, high transmittance, electrochemical stability and the possibility of glycosylation, which provides a simple route for specific biofunctionalization as well as an effective antifouling strategy. Though curcumin has potential treatment value for most chronic diseases, it exerts little potency in the clinic because of its low aqueous solubility, high chemical instability and poor pharmacokinetics. To enhance its potency, we developed a zein-based micelle as a nanocarrier to encapsulate curcumin. Herein, superhydrophilic zwitterionic polymers, poly(sulfobetaine methacrylate) (PSBMA), were conjugated to zein to obtain an amphiphilic zein-PSBMA conjugate. These conjugates could self-assemble into micelles composed of antifouling PSBMA shells and zein cores. The results from the cytokine secretion assay showed that the micelles induced a low level of macrophage activation. Moreover, the results from the in vivo fluorescence imaging experiment confirmed their long-circulating property, exceeding 72 h in mice. In comparison with native curcumin, micelle-encapsulated curcumin had a 230-fold increase in stability in vitro, and its half-life was 22-fold longer, according to a pharmacokinetic study on mice. Overall, this work presents a zein-PSBMA micelle with a long circulation time as a useful nanocarrier for effective curcumin delivery. The apatite formation and in-vitro biocompatibility of Ti-29Nb-14Ta-4.5Zr (TNTZ) alloy reinforced by various nano-sized phases of α″, α, and ω in the β matrix have been studied. The electrochemical performances of the elaborated microstructures have been assessed through potentiodynamic polarization in the simulated body fluid (SBF) and interestingly, the β + ω specimen exhibited an extraordinary corrosion resistance compared to the others. This was attributed to the uniform distribution, spherical morphology and coherent interface of the ω nano-precipitates. The polarization tests in simulated body fluid showed the high tendency of apatite formation on the surface of the β- matrix contained ω precipitates. The in-vitro cytotoxicity analysis employing MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed >85% cell viability of the TNTZ alloy reinforced by nano-ω precipitations. Since this specimen showed the highest cell adhesion as well, it introduces this structure as a promising high potential candidate for biomedical applications due to its high corrosion resistance, biocompatibility, ultra-low cytotoxicity, and good cell adhesion. This study investigates stabilization of graphene oxide (GO) nanosheets in polyethylene oxide-polypropylene oxide (PEO-PPO) block copolymers (P103, P123 and F127). Changes in micellization of copolymers upon GO addition were monitored using dynamic light (DLS) and small angle neutron scattering (SANS). find more Structural developments at sheet surface were studied with two possibilities; (i) adsorption of PPO block over hydrophobic basal plane allowing the engagement of hydrophilic PEO with aqueous bulk, and (ii) adsorption of micelles mediated via carboxylated groups. Insignificant changes in micellar parameters for P123 and P127 were indicative of their inferior interaction with GO. On the other hand, P103 micelles exhibited high affinity for sheets, noticeable as emergence of mass fractals and more than two-fold enhancement in micelle number density. The latter allowed coverage of entire surface with P103 micelles. Existence of mass fractals was verified by extracting the form and structure factors from the fitted SANS data.